Rabbit Recombinant Monoclonal Histone H4 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Synthetic Peptide within Human H4C1.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 antibody [HL1977] (AB308404), expandable thumbnail](https://content.abcam.com/products/images/histone-h4-antibody-hl1977-ab308404--immunocytochemistry-immunofluorescence-img408184.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 antibody [HL1977] (AB308404)")
![Immunohistochemistry paraffin embedded sections - Anti-Histone H4 antibody [HL1977] (AB308404), expandable thumbnail](https://content.abcam.com/products/images/histone-h4-antibody-hl1977-ab308404--immunohistochemistry-paraffin-embedded-sections-img408180.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry paraffin embedded sections - Anti-Histone H4 antibody [HL1977] (AB308404)")
![Immunohistochemistry paraffin embedded sections - Anti-Histone H4 antibody [HL1977] (AB308404), expandable thumbnail](https://content.abcam.com/products/images/histone-h4-antibody-hl1977-ab308404--immunohistochemistry-paraffin-embedded-sections-img408179.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry paraffin embedded sections - Anti-Histone H4 antibody [HL1977] (AB308404)")
![Western blot - Anti-Histone H4 antibody [HL1977] (AB308404), expandable thumbnail](https://content.abcam.com/products/images/histone-h4-antibody-hl1977-ab308404--western-blot-img408183.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Histone H4 antibody [HL1977] (AB308404)")
![Western blot - Anti-Histone H4 antibody [HL1977] (AB308404), expandable thumbnail](https://content.abcam.com/products/images/histone-h4-antibody-hl1977-ab308404--western-blot-img408181.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Histone H4 antibody [HL1977] (AB308404)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Synthetic Peptide within Human H4C1. The exact immunogen used to generate this antibody is proprietary information. Database link P62805
Reactivity data
Select an application| IHC-P | ICC/IF | WB | |
|---|---|---|---|
| Human | Expected | Tested | Tested |
| Mouse | Tested | Expected | Tested |
| Rat | Tested | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 - 1/20000 | Notes - |
Species Mouse | Dilution info 1/10000 - 1/20000 | Notes - |
Species Rat | Dilution info 1/10000 - 1/20000 | Notes - |
Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alternative names
H4/A, H4FA, HIST1H4A, H4C2, H4/I, H4FI, HIST1H4B, H4C3, H4/G, H4FG, HIST1H4C, H4C4, H4/B, H4FB, HIST1H4D, H4C5, H4/J, H4FJ, HIST1H4E, H4C6, H4/C, H4FC, HIST1H4F, H4C8, H4/H, H4FH, HIST1H4H, H4C9, H4/M, H4FM, HIST1H4I, H4C11, H4/E, H4FE, HIST1H4J, H4C12, H4/D, H4FD, HIST1H4K, H4C13, H4/K, H4FK, HIST1H4L, H4C14, H4/N, H4F2, H4FN, HIST2H4, HIST2H4A, H4C15, H4/O, H4FO, HIST2H4B, H4C16, H4-16, HIST4H4, H4FC, H4/C, H4C8, HIST1H4F, H4C6, HIST1H4E, H4FJ, H4/J, H4C5, HIST1H4D, H4FB, H4/B, H4C4, HIST1H4C, H4FG, H4/G, H4C3, HIST1H4B, H4FI, H4/I, H4C2, HIST1H4A, H4FA, H4/A, H4C1, H4F2, HIST4H4, H4-16, H4C16, H4FO, HIST2H4B, H4/O, H4C15, HIST2H4A, HIST2H4, H4FN, H4/N, H4C14, HIST1H4L, H4FK, H4/K, HIST1H4K, H4/H, H4FH, HIST1H4H, H4C9, H4/M, H4FM, HIST1H4I, H4C11, H4FE, H4C13, H4FD, H4/D, H4C12, HIST1H4J, H4/E, Histone H4
Recommended products
Rabbit Recombinant Monoclonal Histone H4 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Synthetic Peptide within Human H4C1.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Synthetic Peptide within Human H4C1. The exact immunogen used to generate this antibody is proprietary information. Database link P62805
- Carrier free
Yes
- Clone number
HL1977
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 antibody [HL1977] (ab308404), expandable thumbnail](https://content.abcam.com/products/images/histone-h4-antibody-hl1977-ab308404--immunocytochemistry-immunofluorescence-img408184.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 antibody [HL1977] (ab308404)")
Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 antibody [HL1977] (ab308404)
For immunofluorescence analysis, HeLa cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of Histone H4 protein using ab308404 at 1/500 dilution (green). Nuclei (blue) is stained with fluoroshield with DAPI.
![Immunohistochemistry paraffin embedded sections - Anti-Histone H4 antibody [HL1977] (ab308404), expandable thumbnail](https://content.abcam.com/products/images/histone-h4-antibody-hl1977-ab308404--immunohistochemistry-paraffin-embedded-sections-img408180.jpg "Immunohistochemistry paraffin embedded sections - Anti-Histone H4 antibody [HL1977] (ab308404)")
Immunohistochemistry paraffin embedded sections - Anti-Histone H4 antibody [HL1977] (ab308404)
Immunohistochemical analysis of rat liver tissue labeling Histone H4 using ab308404 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min
![Immunohistochemistry paraffin embedded sections - Anti-Histone H4 antibody [HL1977] (ab308404), expandable thumbnail](https://content.abcam.com/products/images/histone-h4-antibody-hl1977-ab308404--immunohistochemistry-paraffin-embedded-sections-img408179.jpg "Immunohistochemistry paraffin embedded sections - Anti-Histone H4 antibody [HL1977] (ab308404)")
Immunohistochemistry paraffin embedded sections - Anti-Histone H4 antibody [HL1977] (ab308404)
Immunohistochemical analysis of mouse brain and stomach tissue labeling Histone H4 using ab308404 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min
![Western blot - Anti-Histone H4 antibody [HL1977] (ab308404), expandable thumbnail](https://content.abcam.com/products/images/histone-h4-antibody-hl1977-ab308404--western-blot-img408183.jpg "Western blot - Anti-Histone H4 antibody [HL1977] (ab308404)")
Western blot - Anti-Histone H4 antibody [HL1977] (ab308404)
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 antibody [HL1977] (ab308404) diluted at 1/20000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
All lanes: Western blot - Anti-Histone H4 antibody [HL1977] (ab308404) at 1/20000 dilution
Lane 1: Neuro2A cell lysate at 30 µg
Lane 2: Rat2 cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
![Western blot - Anti-Histone H4 antibody [HL1977] (ab308404), expandable thumbnail](https://content.abcam.com/products/images/histone-h4-antibody-hl1977-ab308404--western-blot-img408181.jpg "Western blot - Anti-Histone H4 antibody [HL1977] (ab308404)")
Western blot - Anti-Histone H4 antibody [HL1977] (ab308404)
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 antibody [HL1977] (ab308404) diluted at 1/10000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
All lanes: Western blot - Anti-Histone H4 antibody [HL1977] (ab308404) at 1/10000 dilution
Lane 1: HEK293T cell lysate at 30 µg
Lane 2: A431 cell lysate at 30 µg
Lane 3: HeLa cell lysate at 30 µg
Lane 4: HepG2 cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Downloads
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