AB194683

Anti-Histone H4 (asymmetric di methyl R3) antibody

Name: Anti-Histone H4 (asymmetric di methyl R3) antibody (ab194683) | Abcam
Brand: Abcam Limited
SKU: AB194683
Price: 525 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(8 Publications )

Rabbit Polyclonal Histone H4 asymmetric di methyl R3 antibody. Suitable for Dot, WB, IHC-P, ICC/IF and reacts with Synthetic peptide, Human, Rat samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human H4C1 me2aR3.

View Alternative Names

H4/A, H4FA, HIST1H4A, H4C2, H4/I, H4FI, HIST1H4B, H4C3, H4/G, H4FG, HIST1H4C, H4C4, H4/B, H4FB, HIST1H4D, H4C5, H4/J, H4FJ, HIST1H4E, H4C6, H4/C, H4FC, HIST1H4F, H4C8, H4/H, H4FH, HIST1H4H, H4C9, H4/M, H4FM, HIST1H4I, H4C11, H4/E, H4FE, HIST1H4J, H4C12, H4/D, H4FD, HIST1H4K, H4C13, H4/K, H4FK, HIST1H4L, H4C14, H4/N, H4F2, H4FN, HIST2H4, HIST2H4A, H4C15, H4/O, H4FO, HIST2H4B, H4C16, H4-16, HIST4H4, H4C1, Histone H4, H4R3me2, H4R3me, H4R3

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (asymmetric di methyl R3) antibody (AB194683)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (asymmetric di methyl R3) antibody (AB194683)

Immunofluorescence staining of 293T cells stained for Histone H4 (asymmetric di methyl R3 with ab194683. Nuclei are labeled with DAPI (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (asymmetric di methyl R3) antibody (AB194683)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (asymmetric di methyl R3) antibody (AB194683)

Paraffin-embedded human rectal cancer tissue stained for Histone H4 (asymmetric di methyl R3) using ab194683 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (asymmetric di methyl R3) antibody (AB194683)

Paraffin-embedded rat brain tissue stained for Histone H4 (asymmetric di methyl R3) using ab194683 at 1/200 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Histone H4 (asymmetric di methyl R3) antibody (AB194683)

Blocking buffer : 3% nonfat dry milk in TBST.

All lanes:

Western blot - Anti-Histone H4 (asymmetric di methyl R3) antibody (ab194683)

Lane 1:

HeLa cell lysate at 25 µg

Lane 2:

H4 protein at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L)

Predicted band size: 11 kDa

false

Supplier Data

Dot Blot - Anti-Histone H4 (asymmetric di methyl R3) antibody (AB194683)

Dot-blot analysis of all sorts of methylation peptides using ab194683.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

Dot, WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human H4C1 me2aR3. The exact immunogen used to generate this antibody is proprietary information.

P62805

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Rat": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H4C1 me2aR3

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 7:521 PubMed38702540

2024

N(alpha)-acetyltransferase 40-mediated histone acetylation plays an important role in ecdysone regulation of metamorphosis in the red flour beetle, Tribolium castaneum.

Applications

Unspecified application

Species

Unspecified reactive species

Sharath Chandra Gaddelapati,Smitha George,Anilkumar Moola,Karthi Sengodan,Subba Reddy Palli

Experimental & molecular medicine 55:1506-1519 PubMed37394590

2023

Arginine 65 methylation of Neurogenin 3 by PRMT1 is required for pancreatic endocrine development of hESCs.

Applications

Unspecified application

Species

Unspecified reactive species

Gahyang Cho,Kwangbeom Hyun,Jieun Choi,Eunji Shin,Bumsoo Kim,Hail Kim,Jaehoon Kim,Yong-Mahn Han

Basic & clinical pharmacology & toxicology 131:18-33 PubMed35470570

2022

Ribavirin inhibits cell proliferation and metastasis and prolongs survival in soft tissue sarcomas by downregulating both protein arginine methyltransferases 1 and 5.

Applications

Unspecified application

Species

Unspecified reactive species

Qingqing Zhang,Ruiying Yang,Yizhen Tian,Suyin Ge,Xiaojuan Nan,Shihao Zhu,Shuhong Dong,Baolai Zhang

The Journal of clinical investigation 132: PubMed35040433

2022

Wnt activation promotes memory T cell polyfunctionality via epigenetic regulator PRMT1.

Applications

Unspecified application

Species

Unspecified reactive species

Bo-Yi Sung,Yi-Hsin Lin,Qiongman Kong,Pali D Shah,Joan Glick Bieler,Scott Palmer,Kent J Weinhold,Hong-Ru Chang,Hailiang Huang,Robin K Avery,Jonathan Schneck,Yen-Ling Chiu

Cell death & disease 12:1066 PubMed34753906

2021

PRMT3 promotes tumorigenesis by methylating and stabilizing HIF1α in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Zhang,Kexin Wang,Xingbo Feng,Jian Wang,Yali Chu,Chunmeng Jia,Qingsi He,Cheng Chen

International journal of molecular sciences 22: PubMed34360791

2021

Protein Arginine Methyltransferase (PRMT) Inhibitors-AMI-1 and SAH Are Effective in Attenuating Rhabdomyosarcoma Growth and Proliferation in Cell Cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Joanna Janisiak,Patrycja Kopytko,Marta Tkacz,Dorota Rogińska,Magdalena Perużyńska,Bogusław Machaliński,Andrzej Pawlik,Maciej Tarnowski

Scientific reports 9:15925 PubMed31685892

2019

The Protein Arginine Methyltransferases 1 and 5 affect Myc properties in glioblastoma stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Annarita Favia,Luisa Salvatori,Simona Nanni,Lisa K Iwamoto-Stohl,Sergio Valente,Antonello Mai,Fiorella Scagnoli,Rosaria Anna Fontanella,Pierangela Totta,Sergio Nasi,Barbara Illi

The Journal of clinical investigation 126:3961-3980 PubMed27643437

2016

Targeting methyltransferase PRMT5 eliminates leukemia stem cells in chronic myelogenous leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Yanli Jin,Jingfeng Zhou,Fang Xu,Bei Jin,Lijing Cui,Yun Wang,Xin Du,Juan Li,Peng Li,Ruibao Ren,Jingxuan Pan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H4 (asymmetric di methyl R3) antibody

Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (asymmetric di methyl R3) antibody (AB194683)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (asymmetric di methyl R3) antibody (AB194683)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (asymmetric di methyl R3) antibody (AB194683)

Western blot - Anti-Histone H4 (asymmetric di methyl R3) antibody (AB194683)

Dot Blot - Anti-Histone H4 (asymmetric di methyl R3) antibody (AB194683)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (8)

Product promise