Anti-Histone H4 (butyryl K16) antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (butyryl K16) antibody (AB240615)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30 mM sodium butyrate for 4 hours) labeling Histone H4 (butyryl K16) with ab240615 at 1/50 dilution in ICC/IF.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

• ChIP

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ChIP - Anti-Histone H4 (butyryl K16) antibody (AB240615)

HeLa (human epithelial cell line from cervix adenocarcinoma; 4 x 10⁶, treated with 30mM sodium butyrate for 4 hours) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5 μg ab240615 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

• WB

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Western blot - Anti-Histone H4 (butyryl K16) antibody (AB240615)

All lanes:

Western blot - Anti-Histone H4 (butyryl K16) antibody (ab240615) at 1/100 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours

Lane 2:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate, treated (+) with 30mM sodium butyrate for 4 hours

Lane 3:

HEK-293 whole cell lysate, untreated (-)

Lane 4:

K562 whole cell lysate, untreated (-)

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 11 kDa

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