Rabbit Polyclonal Histone H4 di methyl R3 antibody. Suitable for Dot, WB, IHC-P, ICC/IF and reacts with Synthetic peptide, Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human H4C1 di methyl R3.
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Publications
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- Cancer & metabolism 10:222022Tadalafil increases the antitumor activity of 5-FU through inhibiting PRMT5-mediated glycolysis and cell proliferation in colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYao Shen,Pan Zhao,Kewei Dong,Jiajia Wang,Huichen Li,Mengyang Li,Ruikai Li,Suning Chen,Yuxia Shen,Zhiyu Liu,Mianjiao Xie,Peng Shen,Jian ZhangPubMed 36474242
- ACS chemical neuroscience 9:838-8482018Neurodegenerative Disease Proteinopathies Are Connected to Distinct Histone Post-translational Modification Landscapes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaren Chen et. al.PubMed 29243911
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human H4C1 di methyl R3. The exact immunogen used to generate this antibody is proprietary information. Database link P62805
Reactivity data
Select an application| Dot | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Expected | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested | Expected |
| Rat | Expected | Expected | Tested | Expected |
| Synthetic peptide | Tested | Not recommended | Not recommended | Not recommended |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Species Rat | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
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Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alternative names
H4/A, H4FA, HIST1H4A, H4C2, H4/I, H4FI, HIST1H4B, H4C3, H4/G, H4FG, HIST1H4C, H4C4, H4/B, H4FB, HIST1H4D, H4C5, H4/J, H4FJ, HIST1H4E, H4C6, H4/C, H4FC, HIST1H4F, H4C8, H4/H, H4FH, HIST1H4H, H4C9, H4/M, H4FM, HIST1H4I, H4C11, H4/E, H4FE, HIST1H4J, H4C12, H4/D, H4FD, HIST1H4K, H4C13, H4/K, H4FK, HIST1H4L, H4C14, H4/N, H4F2, H4FN, HIST2H4, HIST2H4A, H4C15, H4/O, H4FO, HIST2H4B, H4C16, H4-16, HIST4H4, H4C1, Histone H4, H4R3me2
Recommended products
Rabbit Polyclonal Histone H4 di methyl R3 antibody. Suitable for Dot, WB, IHC-P, ICC/IF and reacts with Synthetic peptide, Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human H4C1 di methyl R3.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human H4C1 di methyl R3. The exact immunogen used to generate this antibody is proprietary information. Database link P62805
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (di methyl R3) antibody (ab194696)
Paraffin-embedded human stomach tissue stained for Histone H4 (di methyl R3) using ab194696 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (di methyl R3) antibody (ab194696)
Immunofluorescence staining of 293T cells stained for Histone H4 (di methyl R3) with ab194696. Nuclei are labeled with DAPI (Blue).
Western blot - Anti-Histone H4 (di methyl R3) antibody (ab194696)
Blocking buffer: 3% nonfat dry milk in TBST.
All lanes: Western blot - Anti-Histone H4 (di methyl R3) antibody (ab194696)
Lane 1: HeLa cell lysate at 25 µg
Lane 2: H4 protein at 25 µg
SecondaryAll lanes: HRP Goat Anti-Rabbit IgG (H+L)
Predicted band size: 11 kDa
Dot Blot - Anti-Histone H4 (di methyl R3) antibody (ab194696)
Dot-blot analysis of methylation peptides using ab194696.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (di methyl R3) antibody (ab194696)
Paraffin-embedded mouse liver tissue stained for Histone H4 (di methyl R3) using ab194696 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (di methyl R3) antibody (ab194696)
Paraffin-embedded rat liver tissue stained for Histone H4 (di methyl R3) using ab194696 at 1/100 dilution in immunohistochemical analysis.
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