Anti-Histone H4 (formyl K91) antibody [EPR18083] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-Histone H4 (formyl K91) antibody [EPR18083] - BSA and Azide free (AB250005)

This data was developed using ab177862, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% BSA/TBST.

All lanes:

Western blot - Anti-Histone H4 (formyl K91) antibody [EPR18083] (<a href='/en-us/products/primary-antibodies/histone-h4-formyl-k91-antibody-epr18083-ab177862'>ab177862</a>) at 1/500 dilution

All lanes:

NIH/3T3 (Mouse embyro fibroblast cells) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 11 kDa

Observed band size: 11 kDa

false

Exposure time: 5s

• PepArr

Unknown

Peptide Array - Anti-Histone H4 (formyl K91) antibody [EPR18083] - BSA and Azide free (AB250005)

This data was developed using ab177862 the same antibody clone in a different buffer formulation.

ab177862 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide : all antigen-containing peptides are displayed as red circles all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.

The complete dataset including full list of all peptides and information on the position of each peptide in the diagram is available under the Product Protocol section.