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AB251356

Anti-Histone H4 (mono methyl R78) antibody [EPR19561] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Histone H4 mono methyl R78 antibody. Carrier free. Suitable for ICC/IF, Dot, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Synthetic peptide samples. Cited in 1 publication.

View Alternative Names

H4/A, H4FA, HIST1H4A, H4C2, H4/I, H4FI, HIST1H4B, H4C3, H4/G, H4FG, HIST1H4C, H4C4, H4/B, H4FB, HIST1H4D, H4C5, H4/J, H4FJ, HIST1H4E, H4C6, H4/C, H4FC, HIST1H4F, H4C8, H4/H, H4FH, HIST1H4H, H4C9, H4/M, H4FM, HIST1H4I, H4C11, H4/E, H4FE, HIST1H4J, H4C12, H4/D, H4FD, HIST1H4K, H4C13, H4/K, H4FK, HIST1H4L, H4C14, H4/N, H4F2, H4FN, HIST2H4, HIST2H4A, H4C15, H4/O, H4FO, HIST2H4B, H4C16, H4-16, HIST4H4, H4C1, Histone H4, H4R78me, H4R78me1, H4R78

4 Images
Flow Cytometry (Intracellular) - Anti-Histone H4 (mono methyl R78) antibody [EPR19561] - BSA and Azide free (AB251356)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Histone H4 (mono methyl R78) antibody [EPR19561] - BSA and Azide free (AB251356)

This data was developed using ab202036, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Histone H4 (mono methyl R78) with ab202036 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (mono methyl R78) antibody [EPR19561] - BSA and Azide free (AB251356)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (mono methyl R78) antibody [EPR19561] - BSA and Azide free (AB251356)

This data was developed using ab202036, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 2% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H4 (mono methyl R78) with ab202036 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on Hela cells.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Western blot - Anti-Histone H4 (mono methyl R78) antibody [EPR19561] - BSA and Azide free (AB251356)
  • WB

Supplier Data

Western blot - Anti-Histone H4 (mono methyl R78) antibody [EPR19561] - BSA and Azide free (AB251356)

This data was developed using ab202036, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Histone H4 (mono methyl R78) antibody [EPR19561] (<a href='/en-us/products/primary-antibodies/histone-h4-mono-methyl-r78-antibody-epr19561-ab202036'>ab202036</a>) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma ) whole cell lysate at 10 µg

Lane 2:

NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 11 kDa

Observed band size: 11 kDa

true

Exposure time: 3min

Dot Blot - Anti-Histone H4 (mono methyl R78) antibody [EPR19561] - BSA and Azide free (AB251356)
  • Dot

Supplier Data

Dot Blot - Anti-Histone H4 (mono methyl R78) antibody [EPR19561] - BSA and Azide free (AB251356)

This data was developed using ab202036, the same antibody clone in a different buffer formulation.

Dot blot analysis of Histone H4 (mono methyl R78) labeled with ab202036 at 1/1000 dilution.

Lane 1 : Histone H4 (mono methyl R78) peptide.

Lane 2 : Histone H4 (asymmetric di methyl R78) peptide.

Lane 3 : Histone H4 (symmetric di methyl R78) peptide.

Lane 4 : Histone H4 unmodified peptide.

Lane 5 : Histone H4 (mono methyl K77) peptide.

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

Exposure time : 3 minutes.

Blocking and dilution buffer : 5% NFDM/TBST.

  • Unconjugated

    Anti-Histone H4 (mono methyl R78) antibody [EPR19561]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19561

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

ICC/IF, WB, Dot, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Primary Antibodies

AB1791

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

Lab Essentials

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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  • 20
Primary Antibodies

AB251365

Anti-Histone H2B (mono methyl K23) antibody [EPR17603] - BSA and Azide free

RabMAb|Advanced Validation|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (mono methyl K23) antibody [EPR17603] - BSA and Azide free (AB251365)

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Primary Antibodies

AB1790

Anti-Histone H2B antibody - ChIP Grade

BOND RX™ Validated

Western blot - Anti-Histone H2B antibody - ChIP Grade (AB1790)

  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Synthetic peptide": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab251356 is the carrier-free version of ab202036.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
See full target information H4C1 meR78
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 22:800 PubMed39731111

2024

Role of PCBP2 in regulating nanovesicles loaded with curcumin to mitigate neuroferroptosis in neural damage caused by heat stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Guo,Yizhan Wu,Guangjun Wang,Jiangwei Liu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com