AB222769

Anti-Histone H4 (phospho S1) antibody [RM194]

Name: Anti-Histone H4 (phospho S1) antibody [RM194] (ab222769) | Abcam
Brand: Abcam Limited
SKU: AB222769
Price: 555 USD
Availability: InStock
Rating: 1 (2 reviews)

Recombinant
What is this?

(2 Reviews)

(1 Publication)

Rabbit Recombinant Monoclonal Histone H4 phospho S1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human H4C1 pS1.

View Alternative Names

H4/A, H4FA, HIST1H4A, H4C2, H4/I, H4FI, HIST1H4B, H4C3, H4/G, H4FG, HIST1H4C, H4C4, H4/B, H4FB, HIST1H4D, H4C5, H4/J, H4FJ, HIST1H4E, H4C6, H4/C, H4FC, HIST1H4F, H4C8, H4/H, H4FH, HIST1H4H, H4C9, H4/M, H4FM, HIST1H4I, H4C11, H4/E, H4FE, HIST1H4J, H4C12, H4/D, H4FD, HIST1H4K, H4C13, H4/K, H4FK, HIST1H4L, H4C14, H4/N, H4F2, H4FN, HIST2H4, HIST2H4A, H4C15, H4/O, H4FO, HIST2H4B, H4C16, H4-16, HIST4H4, H4C1, Histone H4, H4S1p

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (phospho S1) antibody [RM194] (AB222769)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (phospho S1) antibody [RM194] (AB222769)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H4 (red) using ab222769 (2 μg/mL) in ICC/IF.

Actin filaments were labeled with fluorescein phalloidin (green).

Supplier Data

Western blot - Anti-Histone H4 (phospho S1) antibody [RM194] (AB222769)

All lanes:

Western blot - Anti-Histone H4 (phospho S1) antibody [RM194] (ab222769) at 0.5 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

Recombinant Histone H4

Predicted band size: 11 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM194

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human H4C1 pS1. The exact immunogen used to generate this antibody is proprietary information.

P62805

Specificity

ab222769 reacts to Histone H4 phosphorylated at Serine 1. The reactivity is not affected by neighboring Arginine 3 modifications. No cross reactivity with Histone H2A phosphorylated at Serine 1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H4C1 pS1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncogene 41:1536-1549 PubMed35087237

2022

Targeting a splicing-mediated drug resistance mechanism in prostate cancer by inhibiting transcriptional regulation by PKCβ1.

Applications

Unspecified application

Species

Unspecified reactive species

James E Melnyk,Veronica Steri,Hao G Nguyen,Y Christina Hwang,John D Gordan,Byron Hann,Felix Y Feng,Kevan M Shokat

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Histone H4 (phospho S1) antibody [RM194]

Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (phospho S1) antibody [RM194] (AB222769)

Western blot - Anti-Histone H4 (phospho S1) antibody [RM194] (AB222769)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise