Anti-Histone H4 (symmetric di methyl R3) antibody

3 product images

• 

  Western blot - Anti-Histone H4 (symmetric di methyl R3) antibody (ab5823)

  This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab5823 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - Anti-Histone H4 (symmetric di methyl R3) antibody (ab5823) at 1 µg

  All lanes: Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

  Secondary

  All lanes: Western blot - VHH Single Domain Anti-Rabbit IgG Fc (HRP) (VHH Single Domain Anti-Rabbit IgG Fc (HRP) ab191866) at 1/50000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 11 kDa

  Observed band size: 14 kDa

  Exposure time: 3min

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (symmetric di methyl R3) antibody (ab5823)

  
  IHC image of ab5823 staining Histone H4 (symmetric di methyl R3) in human kidney formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5823, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• 

  Peptide Array - Anti-Histone H4 (symmetric di methyl R3) antibody (ab5823)

  All batches of ab5823 are tested in Peptide Array against peptides to different Histone H4 and H2A modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H4 - symmetric di methyl R3 peptide (Human Histone H4 (symmetric di methyl R3) peptide ab14791), indicating that this antibody specifically recognises the Histone H4 - symmetric di methyl R3 modification.

  Human Histone H4 (symmetric di methyl R3) peptide ab14791 - Histone H4 - symmetric di methyl R3

  ab17416 - Histone H4 asymmetric di methyl R3

  ab15821 - Histone H4 - unmodified

  ab22399 - Histone H2A symmetric di methyl R3

  ab22398 - Histone H2A asymmetric di methyl R3

  ab13186 - Histone H2A - unmodified