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AB5823

Anti-Histone H4 (symmetric di methyl R3) antibody

4

(19 Reviews)

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(101 Publications)

Anti-Histone H4 (symmetric di methyl R3) antibody (ab5823) is a rabbit polyclonal antibody detecting Histone H4 in Western Blot, IHC-P, PepArr. Suitable for Arabidopsis thaliana, Cow, Human.

- Over 90 publications
- Trusted since 2004

View Alternative Names

H4/A, H4FA, HIST1H4A, H4C2, H4/I, H4FI, HIST1H4B, H4C3, H4/G, H4FG, HIST1H4C, H4C4, H4/B, H4FB, HIST1H4D, H4C5, H4/J, H4FJ, HIST1H4E, H4C6, H4/C, H4FC, HIST1H4F, H4C8, H4/H, H4FH, HIST1H4H, H4C9, H4/M, H4FM, HIST1H4I, H4C11, H4/E, H4FE, HIST1H4J, H4C12, H4/D, H4FD, HIST1H4K, H4C13, H4/K, H4FK, HIST1H4L, H4C14, H4/N, H4F2, H4FN, HIST2H4, HIST2H4A, H4C15, H4/O, H4FO, HIST2H4B, H4C16, H4-16, HIST4H4, H4C1, Histone H4, H4R3me2

3 Images
Western blot - Anti-Histone H4 (symmetric di methyl R3) antibody (AB5823)
  • WB

Lab

Western blot - Anti-Histone H4 (symmetric di methyl R3) antibody (AB5823)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab5823 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-Histone H4 (symmetric di methyl R3) antibody (ab5823) at 1 µg

All lanes:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Secondary

All lanes:

Western blot - VHH Single Domain Anti-Rabbit IgG Fc (HRP) (<a href='/en-us/products/secondary-antibodies/vhh-single-domain-rabbit-igg-fc-hrp-ab191866'>ab191866</a>) at 1/50000 dilution

Predicted band size: 11 kDa

Observed band size: 14 kDa

true

Exposure time: 3min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (symmetric di methyl R3) antibody (AB5823)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (symmetric di methyl R3) antibody (AB5823)

IHC image of ab5823 staining Histone H4 (symmetric di methyl R3) in human kidney formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5823, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Peptide Array - Anti-Histone H4 (symmetric di methyl R3) antibody (AB5823)
  • PepArr

Lab

Peptide Array - Anti-Histone H4 (symmetric di methyl R3) antibody (AB5823)

All batches of ab5823 are tested in Peptide Array against peptides to different Histone H4 and H2A modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H4 - symmetric di methyl R3 peptide (ab14791), indicating that this antibody specifically recognises the Histone H4 - symmetric di methyl R3 modification.

ab14791 - Histone H4 - symmetric di methyl R3

ab17416 - Histone H4 asymmetric di methyl R3

ab15821 - Histone H4 - unmodified

ab22399 - Histone H2A symmetric di methyl R3

ab22398 - Histone H2A asymmetric di methyl R3

ab13186 - Histone H2A - unmodified

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Cow, Human, Arabidopsis thaliana

Applications

WB, IHC-P, PepArr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "PepArr" : {"fullname" : "Peptide Array", "shortname":"PepArr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "PepArr-species-checked": "guaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" }, "Arabidopsis thaliana": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "PepArr-species-checked": "guaranteed", "PepArr-species-dilution-info": "0.02-0.2 µg/mL", "PepArr-species-notes": "<p></p>" }, "Corn": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" }, "Cow": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "PepArr-species-checked": "guaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" }, "Drosophila melanogaster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" }, "Oncorhynchus mykiss": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" }, "Saccharomyces cerevisiae": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" }, "Synthetic peptide - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "PepArr-species-checked": "testedAndGuaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "<p></p>" }, "Tobacco": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "PepArr-species-checked": "predicted", "PepArr-species-dilution-info": "", "PepArr-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-Histone H4 (symmetric di methyl R3) antibody (ab5823) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Peptide Array in Arabidopsis thaliana, Cow, Human samples.

What is the molecular weight of Histone H4?
Anti-Histone H4 (symmetric di methyl R3) (ab5823) specifically detects a band for Histone H4 (UniProt: P62805) at a molecular weight of 11.5kDa.

Trusted by the scientific community
Anti-Histone H4 (symmetric di methyl R3) (ab5823) was first used in a scientific publication in 2004 and has been cited over 90 times in peer-reviewed journals.

Reviewed by scientists
Anti-Histone H4 (symmetric di methyl R3) (ab5823) has over 20 independent reviews from customers.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
See full target information H4C1 symmetric di methyl R3
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Publications (101)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112846 PubMed40655096

2025

Epigenetic regulation of intracellular branched-chain amino acid homeostasis maintains a normal lifespan.

Applications

Unspecified application

Species

Unspecified reactive species

Sejung Park,Yan Liu,Suji Lim,Hong-Yeoul Ryu,Seong Hoon Ahn

MedComm 6:e70150 PubMed40384988

2025

PRMT5 Promotes Pancreatic Cancer Tumorigenesis via Positive PRMT5/C-Myc Feedback Loop.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Yang,Ping Song,Zhaofeng Xiao,Renyi Su,Xin Fang,Yichao Wu,Xiao Xu,Kai Wang

BMC plant biology 24:188 PubMed38486139

2024

SISTER OF FCA physically associates with SKB1 to regulate flowering time in Arabidopsis thaliana.

Applications

Unspecified application

Species

Unspecified reactive species

Chunhong Qiu,Tengyue Wang,Hui Wang,Zhen Tao,Chuanhong Wang,Jing Ma,Shuai Li,Yibing Zhao,Jifang Liu,Peijin Li

Cancer research communications 3:2233-2243 PubMed37861290

2023

PRMT5 Inhibitors Regulate DNA Damage Repair Pathways in Cancer Cells and Improve Response to PARP Inhibition and Chemotherapies.

Applications

Unspecified application

Species

Unspecified reactive species

Jack Carter,Michael Hulse,Monisha Sivakumar,Jessica Burtell,Venkat Thodima,Min Wang,Anjana Agarwal,Komali Vykuntam,Jacob Spruance,Neha Bhagwat,Joseph Rager,Bruce Ruggeri,Peggy Scherle,Koichi Ito

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2202737 PubMed36941223

2023

The PRMT6/PARP1/CRL4B Complex Regulates the Circadian Clock and Promotes Breast Tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Tianshu Yang,Wei Huang,Tianyu Ma,Xin Yin,Jingyao Zhang,Miaomiao Huo,Ting Hu,Tianyang Gao,Wei Liu,Die Zhang,Hefen Yu,Xu Teng,Min Zhang,Hao Qin,Yunkai Yang,Baowen Yuan,Yan Wang

Journal of medicinal chemistry 65:13793-13812 PubMed36206451

2022

Discovery and Biological Characterization of PRMT5:MEP50 Protein-Protein Interaction Inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew M Asberry,Xinpei Cai,Xuehong Deng,Ulises Santiago,Sheng Liu,Hunter S Sims,Weida Liang,Xueyong Xu,Jun Wan,Wen Jiang,Carlos J Camacho,Mingji Dai,Chang-Deng Hu

Cancers 14: PubMed36230689

2022

Expression, Localization and Prognosis Association of MEP50 in Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Samyuktha Suresh,Mathilde Vinet,Rayan Dakroub,Laetitia Lesage,Mengliang Ye,Hussein Fayyad-Kazan,André Nicolas,Didier Meseure,Thierry Dubois

Nature communications 13:5676 PubMed36167829

2022

TP53 mutations and RNA-binding protein MUSASHI-2 drive resistance to PRMT5-targeted therapy in B-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tatiana Erazo,Chiara M Evans,Daniel Zakheim,Karen L Chu,Alice Yunsi Refermat,Zahra Asgari,Xuejing Yang,Mariana Da Silva Ferreira,Sanjoy Mehta,Marco Vincenzo Russo,Andrea Knezevic,Xi-Ping Zhang,Zhengming Chen,Myles Fennell,Ralph Garippa,Venkatraman Seshan,Elisa de Stanchina,Olena Barbash,Connie Lee Batlevi,Christina S Leslie,Ari M Melnick,Anas Younes,Michael G Kharas

International journal of biological sciences 18:4329-4340 PubMed35864961

2022

PRMT5 acts as a tumor suppressor by inhibiting Wnt/β-catenin signaling in murine gastric tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuling Tang,Lei Dong,Chong Zhang,Xiubin Li,Rongyu Li,Huisang Lin,Yini Qi,Mingchuan Tang,Yanli Peng,Chuan Liu,Jian Zhou,Ning Hou,Wenjia Liu,Guan Yang,Xiao Yang,Yan Teng

Life science alliance 5: PubMed35705491

2022

PRMT1 is required for the generation of MHC-associated microglia and remyelination in the central nervous system.

Applications

Unspecified application

Species

Unspecified reactive species

Jeesan Lee,Oscar David Villarreal,Yu Chang Wang,Jiannis Ragoussis,Serge Rivest,David Gosselin,Stéphane Richard
View all publications
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