Rabbit Polyclonal Histone H4 tri methyl K20 antibody. Suitable for ChIP, WB, IHC-P and reacts with Human, Cow, Recombinant full length protein - Saccharomyces cerevisiae samples. Cited in 201 publications.
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- Cell discovery 9:1202023Structural basis of nucleosomal H4K20 recognition and methylation by SUV420H1 methyltransferase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFolan Lin,Ruxin Zhang,Weihan Shao,Cong Lei,Mingxi Ma,Ying Zhang,Zengqi Wen,Wanqiu LiPubMed 38052811
- Frontiers in cell and developmental biology 11:12931222023A complex interplay between H2A.Z and HP1 isoforms regulates pericentric heterochromatin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJessica González,Laia Bosch-Presegué,Anna Marazuela-Duque,Anna Guitart-Solanes,María Espinosa-Alcantud,Agustín F Fernandez,Jeremy P Brown,Juan Ausió,Berta N Vazquez,Prim B Singh,Mario F Fraga,Alejandro VaqueroPubMed 38020886
- Pharmaceutics 15:2023Epidrugs as Promising Tools to Eliminate Artemisinin-Resistant and Quiescent Parasites.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThibaud Reyser,Lucie Paloque,Michel Nguyen,Jean-Michel Augereau,Matthew John Fuchter,Marie Lopez,Paola B Arimondo,Storm Hassell-Hart,John Spencer,Luisa Di Stefano,Françoise Benoit-VicalPubMed 37896200
- Nature 622:619-6262023Inhibition of fatty acid oxidation enables heart regeneration in adult mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiang Li,Fan Wu,Stefan Günther,Mario Looso,Carsten Kuenne,Ting Zhang,Marion Wiesnet,Stephan Klatt,Sven Zukunft,Ingrid Fleming,Gernot Poschet,Astrid Wietelmann,Ann Atzberger,Michael Potente,Xuejun Yuan,Thomas BraunPubMed 37758950
- Nature communications 14:40812023Histone H1 facilitates restoration of H3K27me3 during DNA replication by chromatin compaction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCuifang Liu,Juan Yu,Aoqun Song,Min Wang,Jiansen Hu,Ping Chen,Jicheng Zhao,Guohong LiPubMed 37429872
- Nature communications 13:69072022Replication collisions induced by de-repressed S-phase transcription are connected with malignant transformation of adult stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing Zhang,Carsten Künne,Dong Ding,Stefan Günther,Xinyue Guo,Yonggang Zhou,Xuejun Yuan,Thomas BraunPubMed 36376321
- Nucleic acids research 50:9212-92252022Lysine 27 dimethylation of Drosophila linker histone dH1 contributes to heterochromatin organization independently of H3K9 methylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJordi Bernués,Andrea Izquierdo-Boulstridge,Oscar Reina,Lucía Castejón,Elena Fernández-Castañer,Núria Leal,Nancy Guerrero-Pepinosa,Carles Bonet-Costa,Olivera Vujatovic,Paula Climent-Cantó,Fernando AzorínPubMed 36039761
- Toxicology in vitro : an international journal published in association with BIBRA 84:1054522022Characterization of primary canine Sertoli cells as a model to test male reproductive toxicant.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZoey Hsuan Hsiao,Lu Li,Xiaozhong Yu,Lei YinPubMed 35931286
- Cells 11:2022Endogenous Retroviral Sequences Behave as Putative Enhancers Controlling Gene Expression through HP1-Regulated Long-Range Chromatin Interactions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSébastien Calvet,Séphora Sallis,Nehmé Saksouk,Cosette Rebouissou,Catherine Teyssier,Annick Lesne,Florence Cammas,Thierry FornéPubMed 35954237
- iScience 25:1043542022Nde1 is required for heterochromatin compaction and stability in neocortical neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlison A Chomiak,Yan Guo,Caroline A Kopsidas,Dennis P McDaniel,Clara C Lowe,Hongna Pan,Xiaoming Zhou,Qiong Zhou,Martin L Doughty,Yuanyi FengPubMed 35601919
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| ChIP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Cow | Expected | Tested | Expected |
| Mammals | Predicted | Predicted | Predicted |
| Recombinant full length protein - Saccharomyces cerevisiae | Not recommended | Tested | Not recommended |
| Saccharomyces cerevisiae | Predicted | Predicted | Predicted |
| Schizosaccharomyces pombe | Predicted | Predicted | Predicted |
| Toxoplasma gondii | Predicted | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted | Predicted |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 4-5 µg/cells for 6 µg/cells | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Mammals, Toxoplasma gondii, Xenopus tropicalis | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Saccharomyces cerevisiae | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Saccharomyces cerevisiae | Dilution info 1 µg/mL | Notes - |
Species Cow | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Mammals, Toxoplasma gondii, Xenopus tropicalis | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Mammals, Toxoplasma gondii, Xenopus tropicalis | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Saccharomyces cerevisiae | Dilution info - | Notes - |
Associated Products
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Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alternative names
Histone H4, HIST2H4, H4FN, H4/E, H4F2, H4/N, HIST2H4A, H4C14, HIST1H4L, H4FK, H4/K, H4C13, HIST1H4K, H4FD, H4/D, H4C12, HIST1H4J, H4FE, HIST1H4H, HIST1H4E, H4FJ, H4/J, H4C5, H4C6, H4/C, H4FC, HIST1H4F, H4C8, H4/H, HIST1H4D, H4FH, H4C15, H4C9, H4/M, H4FM, HIST1H4I, H4C11, HIST4H4, H4-16, H4C16, HIST2H4B, H4FO, H4/O, H4/B, H4FB, H4C1, H4/A, H4FA, HIST1H4A, H4C2, H4/I, HIST1H4B, H4C3, H4/G, H4FG, HIST1H4C, H4C4, H4FI, H4K20me3
Recommended products
Rabbit Polyclonal Histone H4 tri methyl K20 antibody. Suitable for ChIP, WB, IHC-P and reacts with Human, Cow, Recombinant full length protein - Saccharomyces cerevisiae samples. Cited in 201 publications.
Alternative names
Histone H4, HIST2H4, H4FN, H4/E, H4F2, H4/N, HIST2H4A, H4C14, HIST1H4L, H4FK, H4/K, H4C13, HIST1H4K, H4FD, H4/D, H4C12, HIST1H4J, H4FE, HIST1H4H, HIST1H4E, H4FJ, H4/J, H4C5, H4C6, H4/C, H4FC, HIST1H4F, H4C8, H4/H, HIST1H4D, H4FH, H4C15, H4C9, H4/M, H4FM, HIST1H4I, H4C11, HIST4H4, H4-16, H4C16, HIST2H4B, H4FO, H4/O, H4/B, H4FB, H4C1, H4/A, H4FA, HIST1H4A, H4C2, H4/I, HIST1H4B, H4C3, H4/G, H4FG, HIST1H4C, H4C4, H4FI, H4K20me3
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Specificity
Histone H4 tri-methylated at Lys 20.
- Concentration
- Purification notes
Purified using Sulpholink column with specific peptide linked via its cysteine residue
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Mouse: Prominent staining of a subset of centromeric or pericentromeric heterochromatin. Interphase: The cells within the culture show a considerable variability in the intensity of staining with the antibody. The relationship between trimethylation levels and cell cycle have not yet been determined but may be a contributor to the amount of methylation detected in each cell. Heterochromatic regions of the interphase nucleus are the primary sites of trimethylation observed by indirect immunofluroescence. Mitosis: Discrete chromosomal regions are labelled intensely, with lower level fluorescence throughout the remainder of the chromosome arms. Immunofluorescence staining was performed as part of the nuclear antibody characterisation program at www.cellnucleus.com. Trimenthylation at Lys20 of Histone H4 isa common hallmark of human cancer (Fraga et al. 2005).
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Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
This image is courtesy of an anonymous customer review.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade (ab9053)
Immunohistochemical staining of paraffin embedded, PFA fixed, mouse liver using ab9053 at 1/100. Heat mediated antigen retrieval was performed using sodium citrate buffer, pH 6, and the sample was permeabilized with 0.05% Triton X 100. The sample was blocked in 5% BSA for 1 hour at room temperature and was then incubated with primary antibody for 3 hours in PBS/0.05% Triton X 100 at room temperature. An Alexa Fluor® 555 donkey anyti-rabbit was used as the secondary at 1/250.
Western blot - Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade (ab9053)
ab9053 is specific for Histone H4 (tri-methyl K20). This is illustrated in lane 5 where the activity of ab9053 is specifically blocked by the addition of the immunizing peptide (Human Histone H4 (tri methyl K20) peptide ab17567).
All lanes: Western blot - Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade (ab9053) at 1 µg/mL
All lanes: Calf thymus histone lysate
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab6721) at 1/5000 dilution
Predicted band size: 11 kDa
Observed band size: 13 kDa
Western blot - Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade (ab9053)
ab9053 is specific for Histone H4 (tri-methyl K20). This is illustrated in lane 5 where the activity of ab9053 is specifically blocked by the addition of the immunizing peptide (Human Histone H4 (tri methyl K20) peptide ab17567).
All lanes: Western blot - Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade (ab9053) at 1 µg/mL
All lanes: Calf thymus histone lysate
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab6721) at 1/5000 dilution
Predicted band size: 11 kDa
Observed band size: 13 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade (ab9053)
IHC image of Histone H4 (tri methyl K20) staining in Human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9053, 0.2μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade (ab9053)
Left panel: a fission yeast whole cell extract and recombinant (r) fission yeast histone H4.
Right panel: fission yeast whole cell extracts from strains with only a single wt (h4) or mutant (h4K20R) gene were immunoblotted.
Review by Steven Sanders submitted 13 August 2004
All lanes: Western blot - Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade (ab9053)
Predicted band size: 11 kDa
ChIP - Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade (ab9053)
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 μg of chromatin, 6 μl of ab9053 (blue), and 20 μl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
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