Rabbit Recombinant Monoclonal GAG antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), I-ELISA and reacts with Transfected cell lysate - Human immunodeficiency virus, Recombinant fragment - Human immunodeficiency virus, Transfected cell line - Human immunodeficiency virus samples.
Images
![Indirect ELISA - Anti-HIV1 p55 + p17 antibody [EPR27327-41] - BSA and Azide free (AB308071), expandable thumbnail](https://content.abcam.com/products/images/hiv1-p55-p17-antibody-epr27327-41-bsa-and-azide-free-ab308071--indirect-elisa-img408747.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-HIV1 p55 + p17 antibody [EPR27327-41] - BSA and Azide free (AB308071)")
![Flow Cytometry (Intracellular) - Anti-HIV1 p55 + p17 antibody [EPR27327-41] - BSA and Azide free (AB308071), expandable thumbnail](https://content.abcam.com/products/images/hiv1-p55-p17-antibody-epr27327-41-bsa-and-azide-free-ab308071--flow-cytometry-intracellular-img408748.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-HIV1 p55 + p17 antibody [EPR27327-41] - BSA and Azide free (AB308071)")
![Immunocytochemistry/ Immunofluorescence - Anti-HIV1 p55 + p17 antibody [EPR27327-41] - BSA and Azide free (AB308071), expandable thumbnail](https://content.abcam.com/products/images/hiv1-p55-p17-antibody-epr27327-41-bsa-and-azide-free-ab308071--immunocytochemistry-immunofluorescence-img408749.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-HIV1 p55 + p17 antibody [EPR27327-41] - BSA and Azide free (AB308071)")
![Western blot - Anti-HIV1 p55 + p17 antibody [EPR27327-41] - BSA and Azide free (AB308071), expandable thumbnail](https://content.abcam.com/products/images/hiv1-p55-p17-antibody-epr27327-41-bsa-and-azide-free-ab308071--western-blot-img408750.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HIV1 p55 + p17 antibody [EPR27327-41] - BSA and Azide free (AB308071)")
![Western blot - Anti-HIV1 p55 + p17 antibody [EPR27327-41] - BSA and Azide free (AB308071), expandable thumbnail](https://content.abcam.com/products/images/hiv1-p55-p17-antibody-epr27327-41-bsa-and-azide-free-ab308071--western-blot-img408751.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HIV1 p55 + p17 antibody [EPR27327-41] - BSA and Azide free (AB308071)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | I-ELISA | IHC-P | IP | |
|---|---|---|---|---|---|---|
| Human immunodeficiency virus | Predicted | Predicted | Predicted | Predicted | Not recommended | Not recommended |
| Recombinant fragment - Human immunodeficiency virus | Tested | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Transfected cell line - Human immunodeficiency virus | Not recommended | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Transfected cell lysate - Human immunodeficiency virus | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human immunodeficiency virus, Recombinant fragment - Human immunodeficiency virus | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human immunodeficiency virus | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human immunodeficiency virus | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human immunodeficiency virus | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human immunodeficiency virus | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human immunodeficiency virus, Recombinant fragment - Human immunodeficiency virus | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human immunodeficiency virus | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human immunodeficiency virus | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human immunodeficiency virus, Recombinant fragment - Human immunodeficiency virus | Dilution info - | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human immunodeficiency virus | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human immunodeficiency virus | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human immunodeficiency virus, Transfected cell line - Human immunodeficiency virus | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human immunodeficiency virus, Transfected cell lysate - Human immunodeficiency virus, Recombinant fragment - Human immunodeficiency virus, Transfected cell line - Human immunodeficiency virus | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human immunodeficiency virus, Transfected cell lysate - Human immunodeficiency virus, Recombinant fragment - Human immunodeficiency virus, Transfected cell line - Human immunodeficiency virus | Dilution info - | Notes - |
Target data
Function
Gag polyprotein. Mediates, with Gag-Pol polyprotein, the essential events in virion assembly, including binding the plasma membrane, making the protein-protein interactions necessary to create spherical particles, recruiting the viral Env proteins, and packaging the genomic RNA via direct interactions with the RNA packaging sequence (Psi). Matrix protein p17. Targets the polyprotein to the plasma membrane via a multipartite membrane-binding signal, that includes its myristoylated N-terminus (By similarity). Matrix protein is part of the pre-integration complex. Implicated in the release from host cell mediated by Vpu. Binds to RNA (By similarity). Capsid protein p24. Forms the conical core that encapsulates the genomic RNA-nucleocapsid complex in the virion. Most core are conical, with only 7% tubular. The core is constituted by capsid protein hexamer subunits. The core is disassembled soon after virion entry (By similarity). The capsid promotes immune invasion by cloaking viral DNA from CGAS detection (By similarity). Host restriction factors such as TRIM5-alpha or TRIMCyp bind retroviral capsids and cause premature capsid disassembly, leading to blocks in reverse transcription. Capsid restriction by TRIM5 is one of the factors which restricts HIV-1 to the human species. Host PIN1 apparently facilitates the virion uncoating (By similarity). On the other hand, interactions with PDZD8 or CYPA stabilize the capsid (By similarity). Nucleocapsid protein p7. Encapsulates and protects viral dimeric unspliced genomic RNA (gRNA). Binds these RNAs through its zinc fingers. Acts as a nucleic acid chaperone which is involved in rearangement of nucleic acid secondary structure during gRNA retrotranscription. Also facilitates template switch leading to recombination. As part of the polyprotein, participates in gRNA dimerization, packaging, tRNA incorporation and virion assembly. p6-gag. Plays a role in budding of the assembled particle by interacting with the host class E VPS proteins TSG101 and PDCD6IP/AIP1.
Alternative names
Gag polyprotein, Pr55Gag, gag
Recommended products
Rabbit Recombinant Monoclonal GAG antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), I-ELISA and reacts with Transfected cell lysate - Human immunodeficiency virus, Recombinant fragment - Human immunodeficiency virus, Transfected cell line - Human immunodeficiency virus samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR27327-41
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HIV1 p55 also known as Gag polyprotein plays an important role in the assembly and maturation of the HIV-1 virus. It is expressed in infected host cells and has a molecular weight of approximately 55 kDa. HIV1 p55 serves as a precursor that gets cleaved by the viral protease to form smaller proteins including p17 (matrix protein) p24 (capsid protein) and p7 (nucleocapsid protein). These cleaved proteins are critical for the viral lifecycle. The cleavage of p55 into p17 among others contributes to the structural integrity and virion production of the virus.
- Biological function summary
Gag polyprotein functions as a scaffold for the formation of viral particles. It associates with the inner cell membrane of the infected cell to form virus-like particles. HIV1 p55 is part of a larger complex that involves interactions with the viral RNA and host proteins facilitating the budding of new viral particles from the infected cell. The p17 component also called the HIV-1 Matrix protein is integral in directing Gag to the plasma membrane where assembly occurs.
- Pathways
The HIV1 p55 plays central roles in the viral replication cycle. It participates in pathways that facilitate viral assembly and release. During these processes Gag interacts with proteins such as TSG101 and ALIX which are involved in the endosomal sorting complex required for transport (ESCRT) pathway. This interaction is important for the budding process allowing the nascent viral particles to exit the host cell efficiently.
- Associated diseases and disorders
HIV1 p55 is directly related to Human Immunodeficiency Virus (HIV) infection leading to Acquired Immunodeficiency Syndrome (AIDS). The virus affects the immune system by depleting CD4+ T cells which HIV1 p55 through interactions with proteins like p17 contributes to this pathological effect. Additionally there's a connection to neurocognitive disorders associated with HIV where p17 is implicated in disrupting neural function showcasing its impact beyond immune suppression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Indirect ELISA - Anti-HIV1 p55 + p17 antibody [EPR27327-41] - BSA and Azide free (ab308071), expandable thumbnail](https://content.abcam.com/products/images/hiv1-p55-p17-antibody-epr27327-41-bsa-and-azide-free-ab308071--indirect-elisa-img408747.jpg "Indirect ELISA - Anti-HIV1 p55 + p17 antibody [EPR27327-41] - BSA and Azide free (ab308071)")
Indirect ELISA - Anti-HIV1 p55 + p17 antibody [EPR27327-41] - BSA and Azide free (ab308071)
This data was developed using Anti-HIV1 p55 + p17 antibody [EPR27327-41] ab308070, the same antibody clone in a different buffer formulation.
Indirect ELISA analysis of Anti-HIV1 p55 + p17 antibody [EPR27327-41] ab308070 at 0-1000 ng/ml.
The secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.![Flow Cytometry (Intracellular) - Anti-HIV1 p55 + p17 antibody [EPR27327-41] - BSA and Azide free (ab308071), expandable thumbnail](https://content.abcam.com/products/images/hiv1-p55-p17-antibody-epr27327-41-bsa-and-azide-free-ab308071--flow-cytometry-intracellular-img408748.jpg "Flow Cytometry (Intracellular) - Anti-HIV1 p55 + p17 antibody [EPR27327-41] - BSA and Azide free (ab308071)")
Flow Cytometry (Intracellular) - Anti-HIV1 p55 + p17 antibody [EPR27327-41] - BSA and Azide free (ab308071)
This data was developed using Anti-HIV1 p55 + p17 antibody [EPR27327-41] ab308070, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T cells (human embryonic kidney epithelial cells) transfected with Myc-tagged HIV2-GAG overexpression construct (Left) / HEK-293T cells transfected with Myc-tagged HIV1-GAG overexpression construct (Right) labelling HIV1 p55 + p17 with Anti-HIV1 p55 + p17 antibody [EPR27327-41] ab308070 at 1/500 dilution (Right).
A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Cells were co-stained with anti-myc conjugated to 647.![Immunocytochemistry/ Immunofluorescence - Anti-HIV1 p55 + p17 antibody [EPR27327-41] - BSA and Azide free (ab308071), expandable thumbnail](https://content.abcam.com/products/images/hiv1-p55-p17-antibody-epr27327-41-bsa-and-azide-free-ab308071--immunocytochemistry-immunofluorescence-img408749.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HIV1 p55 + p17 antibody [EPR27327-41] - BSA and Azide free (ab308071)")
Immunocytochemistry/ Immunofluorescence - Anti-HIV1 p55 + p17 antibody [EPR27327-41] - BSA and Azide free (ab308071)
This data was developed using Anti-HIV1 p55 + p17 antibody [EPR27327-41] ab308070, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labelling HIV1 p55 + p17 with Anti-HIV1 p55 + p17 antibody [EPR27327-41] ab308070 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).
Confocal image showing positive staining in HEK-293T cells transfected with a HIV1-GAG expression vector containing a myc tag and no staining in HEK-293T cells transfected with a HIV2-GAG expression vector containing a myc tag.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.![Western blot - Anti-HIV1 p55 + p17 antibody [EPR27327-41] - BSA and Azide free (ab308071), expandable thumbnail](https://content.abcam.com/products/images/hiv1-p55-p17-antibody-epr27327-41-bsa-and-azide-free-ab308071--western-blot-img408750.jpg "Western blot - Anti-HIV1 p55 + p17 antibody [EPR27327-41] - BSA and Azide free (ab308071)")
Western blot - Anti-HIV1 p55 + p17 antibody [EPR27327-41] - BSA and Azide free (ab308071)
This data was developed using 308070, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
Anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 3 secondsAll lanes: Western blot - Anti-HIV1 p55 + p17 antibody [EPR27327-41] (Anti-HIV1 p55 + p17 antibody [EPR27327-41] ab308070) at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector containing a His tag whole cell lysate at 20 µg
Lane 2: 293T cells transfected with a HIV1-GAG p55 expression vector containing a His-tag whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 55 kDa
Exposure time: 3s
![Western blot - Anti-HIV1 p55 + p17 antibody [EPR27327-41] - BSA and Azide free (ab308071), expandable thumbnail](https://content.abcam.com/products/images/hiv1-p55-p17-antibody-epr27327-41-bsa-and-azide-free-ab308071--western-blot-img408751.jpg "Western blot - Anti-HIV1 p55 + p17 antibody [EPR27327-41] - BSA and Azide free (ab308071)")
Western blot - Anti-HIV1 p55 + p17 antibody [EPR27327-41] - BSA and Azide free (ab308071)
This data was developed using 308070, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
This antibody does not cross-react with HIV2-GAG.
Exposure time: 3 secondsAll lanes: Western blot - Anti-HIV1 p55 + p17 antibody [EPR27327-41] (Anti-HIV1 p55 + p17 antibody [EPR27327-41] ab308070) at 1/1000 dilution
Lane 1: His-tagged HIV2-GAG recombinant protein at 10 ng
Lane 2: His-tagged HIV1-GAG p17 fragment at 10 ng
Lane 3: His-tagged HIV1-GAG p24 fragment at 10 ng
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 17 kDa
Exposure time: 3s
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