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Rabbit Recombinant Monoclonal GAG antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), I-ELISA and reacts with Transfected cell lysate - Human immunodeficiency virus, Recombinant fragment - Human immunodeficiency virus, Transfected cell line - Human immunodeficiency virus samples.

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Images

Indirect ELISA - Anti-HIV1 p55 + p24 antibody [EPR27327-79] - BSA and Azide free (AB308073), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-HIV1 p55 + p24 antibody [EPR27327-79] - BSA and Azide free (AB308073), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-HIV1 p55 + p24 antibody [EPR27327-79] - BSA and Azide free (AB308073), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIV1 p55 + p24 antibody [EPR27327-79] - BSA and Azide free (AB308073), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIV1 p55 + p24 antibody [EPR27327-79] - BSA and Azide free (AB308073), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)I-ELISAIP
Human immunodeficiency virus
Predicted
Predicted
Predicted
Predicted
Predicted
Not recommended
Recombinant fragment - Human immunodeficiency virus
Tested
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Transfected cell line - Human immunodeficiency virus
Not recommended
Tested
Tested
Tested
Not recommended
Not recommended
Transfected cell lysate - Human immunodeficiency virus
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Transfected cell lysate - Human immunodeficiency virus, Recombinant fragment - Human immunodeficiency virus

Dilution info

-

Notes

-

Predicted
Predicted

Species

Human immunodeficiency virus

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Transfected cell line - Human immunodeficiency virus

Dilution info

-

Notes

-

Tested
Tested

Species

Transfected cell line - Human immunodeficiency virus

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Human immunodeficiency virus

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate - Human immunodeficiency virus, Recombinant fragment - Human immunodeficiency virus

Dilution info

-

Notes

-

Tested
Tested

Species

Transfected cell line - Human immunodeficiency virus

Dilution info

-

Notes

-

Predicted
Predicted

Species

Human immunodeficiency virus

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate - Human immunodeficiency virus, Recombinant fragment - Human immunodeficiency virus

Dilution info

-

Notes

-

Tested
Tested

Species

Transfected cell line - Human immunodeficiency virus

Dilution info

-

Notes

-

Predicted
Predicted

Species

Human immunodeficiency virus

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate - Human immunodeficiency virus, Recombinant fragment - Human immunodeficiency virus

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant fragment - Human immunodeficiency virus

Dilution info

-

Notes

-

Predicted
Predicted

Species

Human immunodeficiency virus

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate - Human immunodeficiency virus, Transfected cell line - Human immunodeficiency virus

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human immunodeficiency virus, Transfected cell lysate - Human immunodeficiency virus, Recombinant fragment - Human immunodeficiency virus, Transfected cell line - Human immunodeficiency virus

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Version

    Target data

    Alternative names

    Gag polyprotein, Pr55Gag, gag

    Recommended products

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    Rabbit Recombinant Monoclonal GAG antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), I-ELISA and reacts with Transfected cell lysate - Human immunodeficiency virus, Recombinant fragment - Human immunodeficiency virus, Transfected cell line - Human immunodeficiency virus samples.

    Alternative names

    Gag polyprotein, Pr55Gag, gag

    Key facts

    Isotype

    IgG

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogen
    • The exact immunogen used to generate this antibody is proprietary information.
    Carrier free

    Yes

    Clone number

    EPR27327-79

    Purification technique

    Affinity purification Protein A

    Concentration
    Loading...

    Storage

    Shipped at conditions

    Blue Ice

    Appropriate long-term storage conditions

    +4°C

    Notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    Supplementary info

    Activity summary

    The HIV1 p55 + p24 refers to key proteins derived from the Gag polyprotein of HIV-1 a retrovirus causing AIDS. The Gag polyprotein is about 55 kilodaltons (kDa) and undergoes proteolytic cleavage to produce several structural proteins including p24. P24 has an approximate mass of 24 kDa. It forms the viral capsid which encases the viral RNA genome. These proteins are expressed during viral assembly in infected host cells important for the formation of virus-like particles. Western blot assays frequently utilize p24 due to its stable and abundant presence in viral particles.

    Biological function summary

    HIV1 proteins p55 and p24 play essential roles in viral replication and particle assembly. The p55 precursor also known as the Gag polyprotein is important for the organization of viral proteins and RNA into new virions. Upon cleavage the p24 protein forms the core of the virus providing structural integrity. These proteins interact with other viral components during the assembly and maturation processes ensuring efficient viral particle production and release.

    Pathways

    The Gag polyprotein and its resulting products such as p24 are critical in the HIV-1 life cycle pathways. They contribute to the formation and budding of viral particles from the host cell membrane. In these pathways they interact with the host cell machinery and other viral proteins like Pol which is responsible for reverse transcription and integration. The interplay between these proteins ensures the successful replication and spread of the virus.

    Associated diseases and disorders

    HIV1 proteins p55 and p24 have a direct connection to acquired immunodeficiency syndrome (AIDS) caused by HIV-1 infection. The viral load often measured through p24 levels correlates with disease progression and immune system deterioration. The proteins also relate to disorders involving immune system dysfunction due to their involvement in virus assembly and release. They connect with the host's immune proteins like CD4 which the virus uses as a receptor to enter cells leading to immune system weakening.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

    9 product images

    • Indirect ELISA - Anti-HIV1 p55 + p24 antibody [EPR27327-79] - BSA and Azide free (ab308073), expandable thumbnail

      Indirect ELISA - Anti-HIV1 p55 + p24 antibody [EPR27327-79] - BSA and Azide free (ab308073)

      This data was developed using Anti-HIV1 p55 + p24 antibody [EPR27327-79] ab308072, the same antibody clone in a different buffer formulation.

      Indirect ELISA analysis of Anti-HIV1 p55 + p24 antibody [EPR27327-79] ab308072 at 0-1000 ng/ml.

      The secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

    • Flow Cytometry (Intracellular) - Anti-HIV1 p55 + p24 antibody [EPR27327-79] - BSA and Azide free (ab308073), expandable thumbnail

      Flow Cytometry (Intracellular) - Anti-HIV1 p55 + p24 antibody [EPR27327-79] - BSA and Azide free (ab308073)

      This data was developed using Anti-HIV1 p55 + p24 antibody [EPR27327-79] ab308072, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T cells (human embryonic kidney epithelial cells) transfected with Myc-tagged HIV2-GAG overexpression construct (Left) / HEK-293T cells transfected with Myc-tagged HIV1-GAG overexpression construct (Right) labelling HIV1 p55 + p24 with Anti-HIV1 p55 + p24 antibody [EPR27327-79] ab308072 at 1/500 dilution (Right). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
      Cells were co-stained with anti-myc conjugated to 647.

    • Immunocytochemistry/ Immunofluorescence - Anti-HIV1 p55 + p24 antibody [EPR27327-79] - BSA and Azide free (ab308073), expandable thumbnail

      Immunocytochemistry/ Immunofluorescence - Anti-HIV1 p55 + p24 antibody [EPR27327-79] - BSA and Azide free (ab308073)

      This data was developed using Anti-HIV1 p55 + p24 antibody [EPR27327-79] ab308072, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labelling HIV1 p55 + p24 with Anti-HIV1 p55 + p24 antibody [EPR27327-79] ab308072 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).
      Confocal image showing positive staining in HEK-293T cells transfected with a HIV1-GAG expression vector containing a myc tag and no staining in HEK-293T cells transfected with a HIV2-GAG expression vector containing a myc tag.
      Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 dilution (Red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIV1 p55 + p24 antibody [EPR27327-79] - BSA and Azide free (ab308073), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIV1 p55 + p24 antibody [EPR27327-79] - BSA and Azide free (ab308073)

      This data was developed using Anti-HIV1 p55 + p24 antibody [EPR27327-79] ab308072, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded cells labeling HIV1 p55 + p24 with Anti-HIV1 p55 + p24 antibody [EPR27327-79] ab308072 at 1/500 followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on (A) HEK-293T transfected with a HIV1-GAG His tag construct, no staining on (B) HEK-293T transfected with a HIV2-GAG His tag construct and (C) HEK-293T transfected with empty plasmid.

      The section was incubated with Anti-HIV1 p55 + p24 antibody [EPR27327-79] ab308072 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIV1 p55 + p24 antibody [EPR27327-79] - BSA and Azide free (ab308073), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIV1 p55 + p24 antibody [EPR27327-79] - BSA and Azide free (ab308073)

      This data was developed using Anti-HIV1 p55 + p24 antibody [EPR27327-79] ab308072, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling HIV1 p55 + p24 with Anti-HIV1 p55 + p24 antibody [EPR27327-79] ab308072 at 1/500 followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Negative control: no staining on mouse liver.

      The section was incubated with Anti-HIV1 p55 + p24 antibody [EPR27327-79] ab308072 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIV1 p55 + p24 antibody [EPR27327-79] - BSA and Azide free (ab308073), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIV1 p55 + p24 antibody [EPR27327-79] - BSA and Azide free (ab308073)

      This data was developed using Anti-HIV1 p55 + p24 antibody [EPR27327-79] ab308072, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling HIV1 p55 + p24 with Anti-HIV1 p55 + p24 antibody [EPR27327-79] ab308072 at 1/500 followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Negative control: no staining on rat liver.

      The section was incubated with Anti-HIV1 p55 + p24 antibody [EPR27327-79] ab308072 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Western blot - Anti-HIV1 p55 + p24 antibody [EPR27327-79] - BSA and Azide free (ab308073), expandable thumbnail

      Western blot - Anti-HIV1 p55 + p24 antibody [EPR27327-79] - BSA and Azide free (ab308073)

      This data was developed using 308072, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

      Anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

      Exposure time: 3 seconds

      All lanes: Western blot - Anti-HIV1 p55 + p24 antibody [EPR27327-79] (Anti-HIV1 p55 + p24 antibody [EPR27327-79] ab308072) at 1/1000 dilution

      Lane 1: 293T cells transfected with an empty vector containing a His tag whole cell lysate at 20 µg

      Lane 2: 293T cells transfected with a HIV-GAG p55 expression vector containing a His-tag whole cell lysate at 20 µg

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

      Observed band size: 55 kDa

      Exposure time: 3s

      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

      Anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

      Exposure time: 3 seconds

    • Western blot - Anti-HIV1 p55 + p24 antibody [EPR27327-79] - BSA and Azide free (ab308073), expandable thumbnail

      Western blot - Anti-HIV1 p55 + p24 antibody [EPR27327-79] - BSA and Azide free (ab308073)

      This data was developed using 308072, the same antibody clone in a different buffer formulation. 
      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

      Anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

      Exposure time: 3 seconds

      All lanes: Western blot - Anti-HIV1 p55 + p24 antibody [EPR27327-79] (Anti-HIV1 p55 + p24 antibody [EPR27327-79] ab308072) at 1/1000 dilution

      Lane 1: His-tagged HIV2-GAG recombinant protein at 10 ng

      Lane 2: His-tagged HIV1-GAG p17 fragment at 10 ng

      Lane 3: His-tagged HIV1-GAG p24 fragment at 10 ng

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

      Observed band size: 24 kDa

      Exposure time: 3s

      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

      Anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

      Exposure time: 3 seconds

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIV1 p55 + p24 antibody [EPR27327-79] - BSA and Azide free (ab308073), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIV1 p55 + p24 antibody [EPR27327-79] - BSA and Azide free (ab308073)

      This data was developed using Anti-HIV1 p55 + p24 antibody [EPR27327-79] ab308072, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling HIV1 p55 + p24 with Anti-HIV1 p55 + p24 antibody [EPR27327-79] ab308072 at 1/500 followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Negative control: no staining on human cerebrum.

      The section was incubated with Anti-HIV1 p55 + p24 antibody [EPR27327-79] ab308072 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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