Rabbit Polyclonal GAG antibody. Suitable for ELISA, WB and reacts with Human immunodeficiency virus, Transfected cell lysate - Human immunodeficiency virus samples. Cited in 38 publications.
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- Viruses 15:2023Early Emerging SARS-CoV-2 Spike Mutants Are Diversified in Virologic Properties but Elicit Compromised Antibody Responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunhao Fan,Shixiong Li,Yao Zhang,Jihao Zheng,Dongfang Wang,Yunxi Liao,Zhibo Cui,Dongyu Zhao,Dan H Barouch,Jingyou YuPubMed 38140642
- Nature communications 14:42272023HIV-1 promotes ubiquitination of the amyloidogenic C-terminal fragment of APP to support viral replication.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Gu,Marie Boisjoli,Mojgan H NaghaviPubMed 37454116
- Virologica Sinica 38:448-4582023Spastin is required for human immunodeficiency virus-1 efficient replication through cooperation with the endosomal sorting complex required for transport (ESCRT) protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenyuan Shen,Chang Liu,Yue Hu,Qian Ding,Jiabin Feng,Zhou Liu,Xiaohong KongPubMed 37172824
- Antimicrobial agents and chemotherapy 67:e01694222023Antiviral Properties of HIV-1 Capsid Inhibitor GSK878.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChunfu Wang,Haichang Huang,Kirsten Mallon,Lourdes Valera,Kyle Parcella,Mark I Cockett,John F Kadow,Eric P Gillis,Mark Krystal,Robert A FridellPubMed 37039636
- PloS one 18:e02810872023HIV-1 release requires Nef-induced caspase activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJason Segura,Joanna Ireland,Zhongcheng Zou,Gwynne Roth,Julianna Buchwald,Thomas J Shen,Elizabeth Fischer,Susan Moir,Tae-Wook Chun,Peter D SunPubMed 36780482
- Journal of immunological techniques in infectious diseases 12:2023Adjuvant effect of dendritic cells activator Imiquimod in genetic immunization with HIV-1 p55 Gag.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJanaina M Alves,Mikhail Inyushin,Vassiliy Tsytsarev,Joshua A Roldan-Kalil,Eric Miranda-Valentin,Gerónimo Maldonado-Martinez,Karla M Ramos-Feliciano,Robert Hunter-MelladoPubMed 37205236
- Viruses 14:2022The C-Terminal Domain of RNase H and the C-Terminus Amino Acid Residue Regulate Virus Release and Autoprocessing of a Defective HIV-1 Possessing M50I and V151I Changes in Integrase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomozumi Imamichi,Qian Chen,Ming Hao,Weizhong Chang,Jun YangPubMed 36560691
- iScience 25:1056512022Cocaine sensitizes the CD4 T cells for HIV infection by co-stimulating NFAT and AP-1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdhikarimayum Lakhikumar Sharma,Dylan Shaffer,Daniel Netting,Mudit TyagiPubMed 36483012
- Cell reports 39:1109242022Functional properties of the spike glycoprotein of the emerging SARS-CoV-2 variant B.1.1.529.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Wang,Saumya Anang,Sho Iketani,Yicheng Guo,Lihong Liu,Phinikoula S Katsamba,Lawrence Shapiro,David D Ho,Joseph G SodroskiPubMed 35658975
- bioRxiv : the preprint server for biology :2021Neutralization and Stability of SARS-CoV-2 Omicron Variant.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCong Zeng,John P Evans,Panke Qu,Julia Faraone,Yi-Min Zheng,Claire Carlin,Joseph S Bednash,Tongqing Zhou,Gerard Lozanski,Rama Mallampalli,Linda J Saif,Eugene M Oltz,Peter Mohler,Kai Xu,Richard J Gumina,Shan-Lu LiuPubMed 34981053
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6 - 8.5
Preservative: 0.09% Sodium azide
Constituents: Whole serum- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Database link Q79665
Consider this alternative
Reactivity data
Select an application| ELISA | WB | |
|---|---|---|
| Human immunodeficiency virus | Expected | Tested |
| Transfected cell lysate - Human immunodeficiency virus | Not recommended | Tested |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human immunodeficiency virus | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human immunodeficiency virus | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human immunodeficiency virus | Dilution info 1/2000 | Notes - |
Species Transfected cell lysate - Human immunodeficiency virus | Dilution info 1/2000 | Notes - |
Target data
Function
Gag polyprotein. Mediates, with Gag-Pol polyprotein, the essential events in virion assembly, including binding the plasma membrane, making the protein-protein interactions necessary to create spherical particles, recruiting the viral Env proteins, and packaging the genomic RNA via direct interactions with the RNA packaging sequence (Psi). Matrix protein p17. Targets the polyprotein to the plasma membrane via a multipartite membrane-binding signal, that includes its myristoylated N-terminus (By similarity). Matrix protein is part of the pre-integration complex. Implicated in the release from host cell mediated by Vpu. Binds to RNA (By similarity). Capsid protein p24. Forms the conical core that encapsulates the genomic RNA-nucleocapsid complex in the virion. Most core are conical, with only 7% tubular. The core is constituted by capsid protein hexamer subunits. The core is disassembled soon after virion entry (By similarity). The capsid promotes immune invasion by cloaking viral DNA from CGAS detection (By similarity). Host restriction factors such as TRIM5-alpha or TRIMCyp bind retroviral capsids and cause premature capsid disassembly, leading to blocks in reverse transcription. Capsid restriction by TRIM5 is one of the factors which restricts HIV-1 to the human species. Host PIN1 apparently facilitates the virion uncoating (By similarity). On the other hand, interactions with PDZD8 or CYPA stabilize the capsid (By similarity). Nucleocapsid protein p7. Encapsulates and protects viral dimeric unspliced genomic RNA (gRNA). Binds these RNAs through its zinc fingers. Acts as a nucleic acid chaperone which is involved in rearangement of nucleic acid secondary structure during gRNA retrotranscription. Also facilitates template switch leading to recombination. As part of the polyprotein, participates in gRNA dimerization, packaging, tRNA incorporation and virion assembly. p6-gag. Plays a role in budding of the assembled particle by interacting with the host class E VPS proteins TSG101 and PDCD6IP/AIP1.
Additional Targets
gag, gag
Alternative names
Gag polyprotein, Pr55Gag, gag
Recommended products
Rabbit Polyclonal GAG antibody. Suitable for ELISA, WB and reacts with Human immunodeficiency virus, Transfected cell lysate - Human immunodeficiency virus samples. Cited in 38 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6 - 8.5
Preservative: 0.09% Sodium azide
Constituents: Whole serum- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Database link Q79665
- Purity
- Whole antiserum
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
'HIV1 p55' alternatively known as 'p55 HIV' 'p55 tax form' or 'p55.fun' is a precursor polyprotein composed of the matrix protein p17 the capsid protein p24 and other smaller proteins. This polyprotein with a molecular mass of approximately 55 kDa is expressed in HIV-infected cells. It is integral to the HIV virus structure providing the necessary framework for subsequent protein processing and assembly of viral particles. Researchers commonly use products like anti-HIV antibodies and techniques such as western blot for detection and study of this protein.
- Biological function summary
HIV1 p55 serves as an essential component in the assembly and maturation of viral particles. It undergoes cleavage by the viral protease to form the individual proteins including p17 and p24 essential for the structural integrity of the HIV virus. These components play specific roles within the viral life cycle; p24 forms the viral core while p17 is involved in regulating viral RNA packaging. Together they contribute to the membrane budding process completing the virus assembly essential for its replication and infection.
- Pathways
HIV1 p55 participates in critical viral infection pathways including the retroviral replication cycle. Protein processing and maturation by the viral protease involve interactions with other viral proteins like the reverse transcriptase and integrase facilitating the integration of viral DNA into the host genome. This process connects HIV1 p55 indirectly to host cellular pathways such as the MAPK signaling pathway influencing viral replication and pathogenesis within the host immune system.
- Associated diseases and disorders
HIV1 p55 is closely related to HIV/AIDS with its proteins playing central roles in disease progression. The HIV infection leading to acquired immunodeficiency syndrome (AIDS) involves the interplay of viral proteins including p17 and p24 with the host's immune response. Detection of p24 in diagnostic assays remains a standard indicator for assessing HIV infection levels in patients. As part of HIV pathogenesis these proteins heavily influence immune system evasion and persistence within the host presenting potential targets for antiretroviral therapy development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-HIV1 p55 + p24 + p17 antibody (ab63917)
All lanes: Western blot - Anti-HIV1 p55 + p24 + p17 antibody (ab63917) at 1/2000 dilution
Lane 1: Extract of MT4 cells
Lane 2: Extract of MT4 cells infected with HIV1 (LAI strain)
This image is courtesy of a customer review submitted by David Carpentier.Western blot - Anti-HIV1 p55 + p24 + p17 antibody (ab63917)
All lanes: Western blot - Anti-HIV1 p55 + p24 + p17 antibody (ab63917) at 1/2000 dilution
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate transfected with a plasmid expressing HIV1 gag
Lane 2: Untransfected HeLa whole cell lysate
SecondaryAll lanes: Goat anti-rabbit IgG (whole molecule) HRP polymer at 1/50000 dilution
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