Mouse Monoclonal REV antibody. Suitable for WB, ELISA and reacts with Transfected cell line - Human immunodeficiency virus, Recombinant full length protein - Human immunodeficiency virus samples. Cited in 9 publications.
Images
![Western blot - Anti-HIV1 Rev antibody [Rev-6] (AB85529), expandable thumbnail](https://content.abcam.com/products/images/hiv1-rev-antibody-rev-6-ab85529--western-blot-img12273.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HIV1 Rev antibody [Rev-6] (AB85529)")
Publications
Filter by
- Molecular therapy. Methods & clinical development 29:40-572023Packaging cells for lentiviral vectors generated using the cumate and coumermycin gene induction systems and nanowell single-cell cloning.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSophie Broussau,Viktoria Lytvyn,Mélanie Simoneau,Claire Guilbault,Mélanie Leclerc,Nazila Nazemi-Moghaddam,Nathalie Coulombe,Seyyed Mehdy Elahi,Scott McComb,Rénald GilbertPubMed 36936448
- Journal of virology 93:2019The Polar Region of the HIV-1 Envelope Protein Determines Viral Fusion and Infectivity by Stabilizing the gp120-gp41 Association.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWuxun Lu et. al.PubMed 30651369
- mBio 9:2018Splicing Factor 3B Subunit 1 Interacts with HIV Tat and Plays a Role in Viral Transcription and Reactivation from Latency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGeorge B Kyei et. al.PubMed 30401776
- Journal of virology 91:2017Nuclear Export Signal Masking Regulates HIV-1 Rev Trafficking and Viral RNA Nuclear Export.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRyan T Behrens et. al.PubMed 27852860
- Methods in molecular biology (Clifton, N.J.) 1087:103-142013HIV-1 Rev function and RNA nuclear-cytoplasmic export.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlan CochranePubMed 24158817
- Nucleic acids research 41:9471-832013Characterization of novel inhibitors of HIV-1 replication that function via alteration of viral RNA processing and rev function.
- PLoS pathogens 9:e10032412013Digoxin suppresses HIV-1 replication by altering viral RNA processing.
- Nucleic acids research 40:3663-752011Differential effects of hnRNP D/AUF1 isoforms on HIV-1 gene expression.Applications:WBReactive species:Unspecified reactive speciesNicole Lund,Miroslav P Milev,Raymond Wong,Tharmila Sanmuganantham,Kathryn Woolaway,Benoit Chabot,Sherif Abou Elela,Andrew J Mouland,Alan CochranePubMed 22187150
- Retrovirology 8:472011Differential effect of CLK SR Kinases on HIV-1 gene expression: potential novel targets for therapy.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: 2.94% Sodium citrate, 0.05% Glycerol (glycerin, glycerine), 0.00061% Tris
- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| WB | ELISA | |
|---|---|---|
| Recombinant full length protein - Human immunodeficiency virus | Not recommended | Expected |
| Transfected cell line - Human immunodeficiency virus | Expected | Not recommended |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human immunodeficiency virus | Dilution info 1/250.00000 - 1/500.00000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human immunodeficiency virus | Dilution info - | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human immunodeficiency virus | Dilution info 1/100.00000 - 1/200.00000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human immunodeficiency virus | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Escorts unspliced or incompletely spliced viral pre-mRNAs (late transcripts) out of the nucleus of infected cells. These pre-mRNAs carry a recognition sequence called Rev responsive element (RRE) located in the env gene, that is not present in fully spliced viral mRNAs (early transcripts). This function is essential since most viral proteins are translated from unspliced or partially spliced pre-mRNAs which cannot exit the nucleus by the pathway used by fully processed cellular mRNAs. Rev itself is translated from a fully spliced mRNA that readily exits the nucleus. Rev's nuclear localization signal (NLS) binds directly to KPNB1/Importin beta-1 without previous binding to KPNA1/Importin alpha-1. KPNB1 binds to the GDP bound form of RAN (Ran-GDP) and targets Rev to the nucleus. In the nucleus, the conversion from Ran-GDP to Ran-GTP dissociates Rev from KPNB1 and allows Rev's binding to the RRE in viral pre-mRNAs. Rev multimerization on the RRE via cooperative assembly exposes its nuclear export signal (NES) to the surface. Rev can then form a complex with XPO1/CRM1 and Ran-GTP, leading to nuclear export of the complex. Conversion from Ran-GTP to Ran-GDP mediates dissociation of the Rev/RRE/XPO1/RAN complex, so that Rev can return to the nucleus for a subsequent round of export. Beside KPNB1, also seems to interact with TNPO1/Transportin-1, RANBP5/IPO5 and IPO7/RANBP7 for nuclear import. The nucleoporin-like HRB/RIP is an essential cofactor that probably indirectly interacts with Rev to release HIV RNAs from the perinuclear region to the cytoplasm.
Alternative names
Protein Rev, ART/TRS, Anti-repression transactivator, Regulator of expression of viral proteins, rev
Recommended products
Mouse Monoclonal REV antibody. Suitable for WB, ELISA and reacts with Transfected cell line - Human immunodeficiency virus, Recombinant full length protein - Human immunodeficiency virus samples. Cited in 9 publications.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: 2.94% Sodium citrate, 0.05% Glycerol (glycerin, glycerine), 0.00061% Tris
- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- Rev-6
- Purification technique
- Affinity purification Protein A
- Light chain type
- kappa
- Concentration
- Purification notes
Purified from supernatants of hybridoma cell cultures by affinitychromatography based on Protein A.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The HIV-1 Rev protein also known as Regulatory protein Rev is an essential component of the HIV-1 virus. It weighs approximately 13 kDa and plays an important role in the viral replication cycle. HIV-1 Rev is expressed in cells infected by the HIV-1 virus predominantly within the nucleus. It interacts with viral RNA elements known as the Rev Response Elements (RRE) helping in the regulation of viral mRNA during the HIV-1 life cycle. Researchers often use techniques like Western blotting to detect and study this protein due to its significant role in HIV infection.
- Biological function summary
The Rev protein facilitates a critical stage in the gene expression of HIV in infected cells. It aids in the export of intron-containing viral RNA from the nucleus to the cytoplasm ensuring that these RNAs are effectively translated into viral proteins. HIV-1 Rev does not function alone; it forms part of a complex involving other cellular proteins such as exportin-1 (CRM1) that helps in its transport functions. This complex interaction allows the virus to efficiently reproduce by bypassing the normal cellular restrictions on nuclear export.
- Pathways
The HIV-1 Rev protein interacts with pathways involved in viral mRNA transport and gene expression regulation. It is particularly significant in the CRM1-mediated nuclear export pathway. Through this pathway Rev is related to various proteins such as Ran GTPase which is critical for the nucleocytoplasmic transport mechanisms. These pathways are vital for the replication and assembly of new viral particles within HIV-infected cells.
- Associated diseases and disorders
The HIV-1 Rev protein is a central figure in the progression of HIV/AIDS. It controls the expression of viral genes impacting how rapidly and effectively the virus multiplies. In the context of HIV-related diseases Rev influences proteins involved in the host cell's regulatory systems including the p53 tumor suppressor protein which can affect cell cycle and apoptosis. Understanding Rev's function and interactions is important in developing treatments that target and mitigate the progression of HIV/AIDS.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-HIV1 Rev antibody [Rev-6] (ab85529), expandable thumbnail](https://content.abcam.com/products/images/hiv1-rev-antibody-rev-6-ab85529--western-blot-img12273.jpg "Western blot - Anti-HIV1 Rev antibody [Rev-6] (ab85529)")
Image from Wong R et al., Retrovirology. 2011 Jun 17;8:47.Fig 8.; doi:10.1186/1742-4690-8-47; 17 June, 2011, Retrovirology, 8:47Western blot - Anti-HIV1 Rev antibody [Rev-6] (ab85529)
Representative western blot detecting HIV1 Rev using ab85529 at 1/250 dilution. An HRP-conjugated secondary antibody (1/5000) was used.
HeLa cells stably transduced with an inducible Tet-On HIV-1 system, were untreated or treated for 4-5 h with TG009 or chlorhexidine, followed by induction of expression of the endogenous HIV-1 provirus by addition of doxycycline.All lanes: Western blot - Anti-HIV1 Rev antibody [Rev-6] (ab85529)
Predicted band size: 13 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com