Anti-HIV1 Rev antibody [Rev-6]

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-HIV1 Rev antibody [Rev-6] (AB85529)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T cells transfected with a HIV1 Rev M, HIV Rev N, a HIV1 Rev O and HIV2 Rev expression vector labelling HIV Rev with ab85529 at 1/500 dilution. ab85529 was incubated overnight at 4° C, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed (ab150117) at 1/1000 dilution at RT for 45 min. ab9106 Anti-Myc Rabbit polyclonal antibody at 1/200 dilution, was co-incubated with ab85529 overnight at 4° C. Nucleus were visualized using DAPI.

Confocal image showing positive staining in 293T cells transfected with a HIV1 Rev M and HIV Rev N expression vector containing a myc-His-tag®(shown in green). but no staining in 293T cells transfected with a HIV1 Rev O and HIV2 Rev expression vector containing a myc-His-tag®.

• WB

PubMed

Western blot - Anti-HIV1 Rev antibody [Rev-6] (AB85529)

Representative western blot detecting HIV1 Rev using ab85529 at 1/250 dilution. An HRP-conjugated secondary antibody (1/5000) was used.

HeLa cells stably transduced with an inducible Tet-On HIV-1 system, were untreated or treated for 4-5 h with TG009 or chlorhexidine, followed by induction of expression of the endogenous HIV-1 provirus by addition of doxycycline. Please note this image was from a different batch produced using hybridoma method

All lanes:

Western blot - Anti-HIV1 Rev antibody [Rev-6] (ab85529)

Predicted band size: 13 kDa

false

Image from Wong R et al., Retrovirology. 2011 Jun 17;8:47.Fig 8.; doi:10.1186/1742-4690-8-47; 17 June, 2011, Retrovirology, 8:47

• I-ELISA

Lab

Indirect ELISA - Anti-HIV1 Rev antibody [Rev-6] (AB85529)

Indirect ELISA analysis of ab133468 incubated with HIV-1 rev recombinant protein for 60 minutes. The Secondary antibody used was Goat Anti-Rabbit IgG (H+L), alkaline phosphatase-conjugated at 1/1000 dilution dilution.

OD was read at 405nm within 5 minutes.

• WB

Lab

Western blot - Anti-HIV1 Rev antibody [Rev-6] (AB85529)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-HIV1 Rev antibody [Rev-6] (ab85529) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-his-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with HIV1 group M Rev containing a myc-his-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L)

Observed band size: 22 kDa

true

Exposure time: 180s