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AB58652

Anti-HIVEP1 antibody [2417C2 a]

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Mouse Monoclonal HIVEP1 antibody. Suitable for WB and reacts with Recombinant fragment - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HIVEP1.

View Alternative Names

ZNF40, HIVEP1, Zinc finger protein 40, Cirhin interaction protein, Gate keeper of apoptosis-activating protein, Human immunodeficiency virus type I enhancer-binding protein 1, Major histocompatibility complex-binding protein 1, Positive regulatory domain II-binding factor 1, CIRIP, GAAP, HIV-EP1, MBP-1, PRDII-BF1

1 Images
Western blot - Anti-HIVEP1 antibody [2417C2 a] (AB58652)
  • WB

Unknown

Western blot - Anti-HIVEP1 antibody [2417C2 a] (AB58652)

The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.

All lanes:

Western blot - Anti-HIVEP1 antibody [2417C2 a] (ab58652)

All lanes:

immunising recombinant protein fragment

Predicted band size: 297 kDa

Observed band size: 34 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2417C2 a

Isotype

IgG1

Carrier free

No

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human HIVEP1. The exact immunogen used to generate this antibody is proprietary information.

P15822

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
ab58652 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: 1% BSA, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.1136% Disodium hydrogenorthophosphate, 0.03% Tripotassium orthophosphate, 0.0225% Potassium chloride, 0.0204% Potassium phosphate monobasic
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HIVEP1 also known as ZAS1 is a transcription factor with a molecular mass of approximately 310 kDa. It plays a role in regulating gene expression by binding to specific DNA sequences. HIVEP1 exhibits expression in various tissues with higher levels found in immune-related tissues including the spleen and lymph nodes. The interaction with DNA allows HIVEP1 to act as a transcriptional integrator contributing to the control of gene networks involved in immune responses.
Biological function summary

HIVEP1 influences the transcriptional activation of genes important for immune system functioning. It forms part of a multi-protein complex that modulates the expression of genes involved in B and T cell development. The activity of HIVEP1 in the immune response emphasizes its essential role in maintaining immune system integrity and proper lymphocyte function. Through its conserved zinc finger motifs HIVEP1 binds to NF-kB response elements affecting the NF-kB signaling pathway.

Pathways

HIVEP1 interacts with the NF-kB signaling pathway and influences processes of immune regulation. This signaling pathway supports inflammatory responses cell survival and proliferation. HIVEP1's regulation of NF-kB is similar to that of other proteins like IKK and p65. Together they coordinate responses to immune challenges by altering transcriptional programs needed during inflammation and adaptive immunity.

HIVEP1 has connections to autoimmune diseases and certain types of cancers. Dysregulation of HIVEP1 expression significantly affects immune cell function potentially leading to conditions like rheumatoid arthritis. In cancer alterations in HIVEP1 function can contribute to improper cell proliferation and survival highlighting interactions with proteins such as c-Rel. Understanding HIVEP1's role shows the importance of maintaining its normal functioning within the immune system and cell growth pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This protein specifically binds to the DNA sequence 5'-GGGACTTTCC-3' which is found in the enhancer elements of numerous viral promoters such as those of SV40, CMV, or HIV-1. In addition, related sequences are found in the enhancer elements of a number of cellular promoters, including those of the class I MHC, interleukin-2 receptor, and interferon-beta genes. It may act in T-cell activation. Involved in activating HIV-1 gene expression. Isoform 2 and isoform 3 also bind to the IPCS (IRF1 and p53 common sequence) DNA sequence in the promoter region of interferon regulatory factor 1 and p53 genes and are involved in transcription regulation of these genes. Isoform 2 does not activate HIV-1 gene expression. Isoform 2 and isoform 3 may be involved in apoptosis.
See full target information HIVEP1
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