Rabbit Polyclonal HJURP antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human HJURP aa 650-700.
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- International journal of molecular sciences 23:2022Unraveling the Role of Histone Variant CENP-A and Chaperone HJURP Expression in Thymic Epithelial Neoplasms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGeorgia Levidou,Konstantinos Palamaris,Alexandros G Sykaras,Georgios Andreadakis,Christos Masaoutis,Irene Theochari,Penelope Korkolopoulou,Dimitra Rontogianni,Stamatios TheocharisPubMed 35955489
- Frontiers in oncology 12:9725502022The expression, clinical relevance, and prognostic significance of HJURP in cholangiocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Yang,Jinyan Yuan,Zhenzhong Liu,Wenwen Cao,Pei LiuPubMed 35965590
- Cell death & disease 12:5832021HJURP promotes proliferation in prostate cancer cells through increasing CDKN1A degradation via the GSK3β/JNK signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjie Lai,Weian Zhu,Chutian Xiao,Xiaojuan Li,Yu Wang,Yuefu Han,Jiayu Zheng,Yingqiu Li,Mingqiang Li,Xingqiao WenPubMed 34099634
- Oncology reports 44:1246-12542020Holliday junction‑recognition protein modulates apoptosis, cell cycle arrest and reactive oxygen species stress in human renal cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiang-Shui Yuan,Zeng-Sheng Chen,Ke Wang,Zong-Liang ZhangPubMed 32582972
- Nature communications 10:40462019Mechanism of centromere recruitment of the CENP-A chaperone HJURP and its implications for centromere licensing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongqing Pan,Kai Walstein,Annika Take,David Bier,Nadine Kaiser,Andrea MusacchioPubMed 31492860
- The Journal of cell biology 213:415-242016Diaphanous formin mDia2 regulates CENP-A levels at centromeres.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenshu Liu et. al.PubMed 27185834
- Molecular oncology 9:657-742014The histone chaperone HJURP is a new independent prognostic marker for luminal A breast carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRocío Montes de Oca,Zachary A Gurard-Levin,Frédérique Berger,Haniya Rehman,Elise Martel,Armelle Corpet,Leanne de Koning,Isabelle Vassias,Laurence O W Wilson,Didier Meseure,Fabien Reyal,Alexia Savignoni,Bernard Asselain,Xavier Sastre-Garau,Geneviève AlmouzniPubMed 25497280
- BioMed research international 2014:1597652014New biomarkers to predict the evolution of in situ breast cancers.Applications:IHCReactive species:HumanS Bravaccini,M M Tumedei,E Scarpi,W Zoli,C Rengucci,L Serra,A Curcio,F Buggi,S Folli,A Rocca,R Maltoni,M Puccetti,D Amadori,R SilvestriniPubMed 25243117
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human HJURP aa 650-700. The exact immunogen used to generate this antibody is proprietary information. Database link Q8NCD3
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Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000 | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2-5 µg/mg of lysate | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04 µg/mL | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Prevents CENPA-H4 tetramerization and prevents premature DNA binding by the CENPA-H4 tetramer. Directly binds Holliday junctions.
Alternative names
FAKTS, FLEG1, URLC9, HJURP, FAKTS, FLEG1, URLC9, Holliday junction recognition protein, 14-3-3-associated AKT substrate, Fetal liver-expressing gene 1 protein, Up-regulated in lung cancer 9
Recommended products
Rabbit Polyclonal HJURP antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human HJURP aa 650-700.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human HJURP aa 650-700. The exact immunogen used to generate this antibody is proprietary information. Database link Q8NCD3
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
Purified using an epitope specific to HJURP immobilized on solid support.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HJURP antibody (ab100800)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling HJURP with ab100800 at 1/5000 (0.2μg/mg). Detection: DAB.
Western blot - Anti-HJURP antibody (ab100800)
All lanes: Western blot - Anti-HJURP antibody (ab100800) at 0.04 µg/mL
Lane 1: K-562 whole cell lysate at 2 µg
Lane 2: U2OS whole cell lysate at 2 µg
Lane 3: HeLa whole cell lysate at 2 µg
Lane 4: MCF-7 whole cell lysate at 2 µg
Predicted band size: 84 kDa
Immunoprecipitation - Anti-HJURP antibody (ab100800)
ab100800 at 1 µg/ml staining HJURP in HeLa cell lysate immunoprecipitated using ab100800 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Exposure time: 30 secondsAll lanes: Immunoprecipitation - Anti-HJURP antibody (ab100800)
Predicted band size: 84 kDa
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