Rabbit Polyclonal HJURP antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HJURP aa 1-200.
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- Stem cell research & therapy 10:822019In vitro expansion affects the response of human bone marrow stromal cells to irradiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Xiang et. al.PubMed 30850008
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human HJURP aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q8NCD3
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Associated Products
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Target data
Function
Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Prevents CENPA-H4 tetramerization and prevents premature DNA binding by the CENPA-H4 tetramer. Directly binds Holliday junctions.
Alternative names
Holliday junction recognition protein, 14-3-3-associated AKT substrate, Fetal liver-expressing gene 1 protein, Up-regulated in lung cancer 9, HJURP, FAKTS, FLEG1, URLC9
Recommended products
Rabbit Polyclonal HJURP antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HJURP aa 1-200.
Alternative names
Holliday junction recognition protein, 14-3-3-associated AKT substrate, Fetal liver-expressing gene 1 protein, Up-regulated in lung cancer 9, HJURP, FAKTS, FLEG1, URLC9
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human HJURP aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q8NCD3
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HJURP also known as Holliday junction recognition protein plays an important mechanical role in chromatin assembly. This protein weighs approximately 82 kDa and coordinates the correct loading of CENP-A at the centromeres during S phase and early G1 phase of the cell cycle. HJURP is mostly expressed in proliferative tissues and cell lines reflecting its involvement in processes requiring accurate chromosomal segregation during cell division. Its presence ensures that centromere identity is passed onto the daughter cells.
- Biological function summary
HJURP is critical for centromere function and stability. It operates by forming a complex with centromere protein A (CENP-A) which completes the assembly and maintenance of centromere chromatin. HJURP achieves this by acting as a chaperone specifically ensuring the accurate deposition of CENP-A at the centromeres. The functionality of HJURP within this complex highlights its role in sustaining genomic integrity throughout cell division processes.
- Pathways
HJURP lies at the center of the centromere-specific chromatin assembly pathway. This pathway is essential for the replenishment of centromeric CENP-A thereby ensuring accurate chromosome segregation. HJURP interacts significantly with other proteins such as CALM and H4 aiding in chromatin dynamics and stability. These interactions help integrate HJURP's actions into larger cellular and genetic processes particularly those involving DNA replication and cell cycle progression.
- Associated diseases and disorders
HJURP links closely to cancerous transformations and chromosomal instability disorders. Abnormal expression or mutations of HJURP can disrupt centromere function potentially leading to aneuploidy—a condition often found in various cancers. Its association with other proteins such as HJURP's interaction with overexpressed CENP-A in these pathological states highlights its significance in maintaining cellular health. Therefore HJURP emerges as a potential biomarker for these conditions and warrants further research into therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Immunocytochemistry/ Immunofluorescence - Anti-HJURP antibody (ab224076)
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for HJURP (green) using ab224076 (4 μg/ml) in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HJURP antibody (ab224076)
Paraffin embedded human stomach tissue stained for HJURP with ab224076 (1/200 dilution) in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HJURP antibody (ab224076)
Paraffin embedded human skin tissue stained for HJURP with ab224076 (1/200 dilution) in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HJURP antibody (ab224076)
Paraffin embedded human testis tissue stained for HJURP with ab224076 (1/200 dilution) in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HJURP antibody (ab224076)
Paraffin embedded human lymph node tissue stained for HJURP with ab224076 (1/200 dilution) in immunohistochemical analysis.
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