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Rabbit Recombinant Monoclonal HJURP antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-HJURP antibody [EPR22619-41] (AB233541), expandable thumbnail
  • Western blot - Anti-HJURP antibody [EPR22619-41] (AB233541), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HJURP antibody [EPR22619-41] (AB233541), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HJURP antibody [EPR22619-41] (AB233541), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IFIHC-P
Human
Not recommended
Not recommended
Tested
Not recommended
Tested

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Prevents CENPA-H4 tetramerization and prevents premature DNA binding by the CENPA-H4 tetramer. Directly binds Holliday junctions.

Alternative names

FAKTS, FLEG1, URLC9, HJURP, Holliday junction recognition protein, 14-3-3-associated AKT substrate, Fetal liver-expressing gene 1 protein, Up-regulated in lung cancer 9

Recommended products

Rabbit Recombinant Monoclonal HJURP antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22619-41
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

HJURP also known as Holliday junction recognition protein plays an important mechanical role in chromatin assembly. This protein weighs approximately 82 kDa and coordinates the correct loading of CENP-A at the centromeres during S phase and early G1 phase of the cell cycle. HJURP is mostly expressed in proliferative tissues and cell lines reflecting its involvement in processes requiring accurate chromosomal segregation during cell division. Its presence ensures that centromere identity is passed onto the daughter cells.

Biological function summary

HJURP is critical for centromere function and stability. It operates by forming a complex with centromere protein A (CENP-A) which completes the assembly and maintenance of centromere chromatin. HJURP achieves this by acting as a chaperone specifically ensuring the accurate deposition of CENP-A at the centromeres. The functionality of HJURP within this complex highlights its role in sustaining genomic integrity throughout cell division processes.

Pathways

HJURP lies at the center of the centromere-specific chromatin assembly pathway. This pathway is essential for the replenishment of centromeric CENP-A thereby ensuring accurate chromosome segregation. HJURP interacts significantly with other proteins such as CALM and H4 aiding in chromatin dynamics and stability. These interactions help integrate HJURP's actions into larger cellular and genetic processes particularly those involving DNA replication and cell cycle progression.

Associated diseases and disorders

HJURP links closely to cancerous transformations and chromosomal instability disorders. Abnormal expression or mutations of HJURP can disrupt centromere function potentially leading to aneuploidy—a condition often found in various cancers. Its association with other proteins such as HJURP's interaction with overexpressed CENP-A in these pathological states highlights its significance in maintaining cellular health. Therefore HJURP emerges as a potential biomarker for these conditions and warrants further research into therapeutic interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-HJURP antibody [EPR22619-41] (ab233541), expandable thumbnail

    Western blot - Anti-HJURP antibody [EPR22619-41] (ab233541)

    Blocking and dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 23771058).

    All lanes: Western blot - Anti-HJURP antibody [EPR22619-41] (ab233541) at 1/1000 dilution

    All lanes: HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 84 kDa

    Observed band size: 84 kDa

    Exposure time: 3min

  • Western blot - Anti-HJURP antibody [EPR22619-41] (ab233541), expandable thumbnail

    Western blot - Anti-HJURP antibody [EPR22619-41] (ab233541)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lanes 1-3: 48 seconds; Lane 4: 3.25 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 23771058).

    Lanes 1-3 in this blot were developed using a higher sensitivity ECL substrate.

    All lanes: Western blot - Anti-HJURP antibody [EPR22619-41] (ab233541) at 1/1000 dilution

    Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 2: HeLa nuclear fraction at 20 µg

    Lane 3: HeLa cytoplasmic fraction at 20 µg

    Lane 4: Human tonsil tissue lysate at 20 µg

    Secondary

    Lanes 1 - 3: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Lane 4: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 84 kDa

    Observed band size: 84 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HJURP antibody [EPR22619-41] (ab233541), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HJURP antibody [EPR22619-41] (ab233541)

    Immunohistochemical analysis of paraffin-embeddded human tonsil tissue labeling HJURP with ab233541 at 1/100 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining in human tonsil germinal center is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    The section was incubated with ab233541 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HJURP antibody [EPR22619-41] (ab233541), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HJURP antibody [EPR22619-41] (ab233541)

    Immunohistochemical analysis of paraffin-embeddded human thymus tissue labeling HJURP with ab233541 at 1/100 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on thymocytes of human thymus cortex (PMID: 17256767) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    The section was incubated with ab233541 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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