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AB255698

Anti-HJURP antibody [EPR22619-41] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal HJURP antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

FAKTS, FLEG1, URLC9, HJURP, Holliday junction recognition protein, 14-3-3-associated AKT substrate, Fetal liver-expressing gene 1 protein, Up-regulated in lung cancer 9

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HJURP antibody [EPR22619-41] - BSA and Azide free (AB255698)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HJURP antibody [EPR22619-41] - BSA and Azide free (AB255698)

Immunohistochemical analysis of paraffin-embeddded human thymus tissue labeling HJURP with ab233541 at 1/100 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on thymocytes of human thymus cortex (PMID : 17256767) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

The section was incubated with ab233541 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233541).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HJURP antibody [EPR22619-41] - BSA and Azide free (AB255698)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HJURP antibody [EPR22619-41] - BSA and Azide free (AB255698)

Immunohistochemical analysis of paraffin-embeddded human tonsil tissue labeling HJURP with ab233541 at 1/100 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in human tonsil germinal center is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

The section was incubated with ab233541 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233541).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22619-41

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab255698 is the carrier-free version of ab233541.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HJURP also known as Holliday junction recognition protein plays an important mechanical role in chromatin assembly. This protein weighs approximately 82 kDa and coordinates the correct loading of CENP-A at the centromeres during S phase and early G1 phase of the cell cycle. HJURP is mostly expressed in proliferative tissues and cell lines reflecting its involvement in processes requiring accurate chromosomal segregation during cell division. Its presence ensures that centromere identity is passed onto the daughter cells.
Biological function summary

HJURP is critical for centromere function and stability. It operates by forming a complex with centromere protein A (CENP-A) which completes the assembly and maintenance of centromere chromatin. HJURP achieves this by acting as a chaperone specifically ensuring the accurate deposition of CENP-A at the centromeres. The functionality of HJURP within this complex highlights its role in sustaining genomic integrity throughout cell division processes.

Pathways

HJURP lies at the center of the centromere-specific chromatin assembly pathway. This pathway is essential for the replenishment of centromeric CENP-A thereby ensuring accurate chromosome segregation. HJURP interacts significantly with other proteins such as CALM and H4 aiding in chromatin dynamics and stability. These interactions help integrate HJURP's actions into larger cellular and genetic processes particularly those involving DNA replication and cell cycle progression.

HJURP links closely to cancerous transformations and chromosomal instability disorders. Abnormal expression or mutations of HJURP can disrupt centromere function potentially leading to aneuploidy—a condition often found in various cancers. Its association with other proteins such as HJURP's interaction with overexpressed CENP-A in these pathological states highlights its significance in maintaining cellular health. Therefore HJURP emerges as a potential biomarker for these conditions and warrants further research into therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Prevents CENPA-H4 tetramerization and prevents premature DNA binding by the CENPA-H4 tetramer. Directly binds Holliday junctions.
See full target information HJURP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica communications 13:86 PubMed40307956

2025

Prion replication in organotypic brain slice cultures is distinct from in vivo inoculation and is species dependent.

Applications

Unspecified application

Species

Unspecified reactive species

Jessy A Slota,Lise Lamoureux,Jennifer Myskiw,Kathy L Frost,Sarah J Medina,Dominic M S Kielich,Melanie Leonhardt,Gunjan Thapar,Ben A Bailey-Elkin,Stephanie A Booth
View all publications
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Product promise

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