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Rabbit Multiclonal HJURP antibody. Suitable for ICC/IF, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HJURP.

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Images

Western blot - Anti-HJURP antibody [RP23040269] (AB308103), expandable thumbnail
  • Western blot - Anti-HJURP antibody [RP23040269] (AB308103), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-HJURP antibody [RP23040269] (AB308103), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS

Form

Liquid

Clonality

Multiclonal

Immunogen

  • Recombinant Fragment Protein within Human HJURP. Database link Q8NCD3

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWB
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/100

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Target data

Function

Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Prevents CENPA-H4 tetramerization and prevents premature DNA binding by the CENPA-H4 tetramer. Directly binds Holliday junctions.

Alternative names

FAKTS, FLEG1, URLC9, HJURP, FAKTS, FLEG1, URLC9, Holliday junction recognition protein, 14-3-3-associated AKT substrate, Fetal liver-expressing gene 1 protein, Up-regulated in lung cancer 9

Recommended products

Rabbit Multiclonal HJURP antibody. Suitable for ICC/IF, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HJURP.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Multiclonal

Immunogen
  • Recombinant Fragment Protein within Human HJURP. Database link Q8NCD3
Clone number

RP23040269

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.

Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

HJURP also known as Holliday junction recognition protein plays an important mechanical role in chromatin assembly. This protein weighs approximately 82 kDa and coordinates the correct loading of CENP-A at the centromeres during S phase and early G1 phase of the cell cycle. HJURP is mostly expressed in proliferative tissues and cell lines reflecting its involvement in processes requiring accurate chromosomal segregation during cell division. Its presence ensures that centromere identity is passed onto the daughter cells.

Biological function summary

HJURP is critical for centromere function and stability. It operates by forming a complex with centromere protein A (CENP-A) which completes the assembly and maintenance of centromere chromatin. HJURP achieves this by acting as a chaperone specifically ensuring the accurate deposition of CENP-A at the centromeres. The functionality of HJURP within this complex highlights its role in sustaining genomic integrity throughout cell division processes.

Pathways

HJURP lies at the center of the centromere-specific chromatin assembly pathway. This pathway is essential for the replenishment of centromeric CENP-A thereby ensuring accurate chromosome segregation. HJURP interacts significantly with other proteins such as CALM and H4 aiding in chromatin dynamics and stability. These interactions help integrate HJURP's actions into larger cellular and genetic processes particularly those involving DNA replication and cell cycle progression.

Associated diseases and disorders

HJURP links closely to cancerous transformations and chromosomal instability disorders. Abnormal expression or mutations of HJURP can disrupt centromere function potentially leading to aneuploidy—a condition often found in various cancers. Its association with other proteins such as HJURP's interaction with overexpressed CENP-A in these pathological states highlights its significance in maintaining cellular health. Therefore HJURP emerges as a potential biomarker for these conditions and warrants further research into therapeutic interventions.

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3 product images

  • Western blot - Anti-HJURP antibody [RP23040269] (ab308103), expandable thumbnail

    Western blot - Anti-HJURP antibody [RP23040269] (ab308103)

    Western blot analysis was performed on modified whole cell extracts (30 µg lysate) of HeLa (Lane 1), T98G (Lane 2), U-87 MG (Lane 3) and U-2 OS (Lane 4). The blot was probed with ab308103 at 1:500 dilution and detected by chemiluminescence using a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1:4000 dilution. A ~83 kDa band corresponding to HJURP was observed across the cell lines tested.

    All lanes: Western blot - Anti-HJURP antibody [RP23040269] (ab308103) at 1/500 dilution

    Lane 1: HeLa whole cell lysate at 30 µg

    Lane 2: T98G whole cell lysate at 30 µg

    Lane 3: U-87 MG whole cell lysate at 30 µg

    Lane 4: U-2 OS whole cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1/4000 dilution

    Developed using the ECL technique.

    Observed band size: 83 kDa

  • Western blot - Anti-HJURP antibody [RP23040269] (ab308103), expandable thumbnail

    Western blot - Anti-HJURP antibody [RP23040269] (ab308103)

    Knockdown of HJURP was achieved by transfecting A549 cells with HJURP specific siRNA. Western blot analysis was performed using modified whole cell extracts (1% SDS) from HJURP knockdown cells (Lane 2) and non-specific scrambled siRNA transfected cells (Lane 1). The blot was probed with ab308103 at 1:500 dilution and a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1:4000 dilution. Loss of signal upon siRNA mediated knockdown confirms that antibody is specific to HJURP.

    All lanes: Western blot - Anti-HJURP antibody [RP23040269] (ab308103) at 1/500 dilution

    Lane 1: non-specific scrambled siRNA transfected A549 cells

    Lane 2: HJURP knockdown A549 cells

    Secondary

    All lanes: HRP-conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1/4000 dilution

    Observed band size: 83 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-HJURP antibody [RP23040269] (ab308103), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-HJURP antibody [RP23040269] (ab308103)

    For immunofluorescence analysis, A549 cells were fixed and permeabilized for detection of endogenous HJURP using ab308103 at 1/100 dilution and labeled with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution.

    Panel a) shows representative cells that were stained for detection and localization of HJURP protein (green)

    Panel b) is stained for nuclei (blue) using DAPI

    Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin at 1/300 dilution

    Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of HJURP

    Panel e) represents control cells with no primary antibody to assess background

    The images were captured at 60X magnification

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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