AB228729

Anti-HKDC1 antibody

Name: Anti-HKDC1 antibody (ab228729) | Abcam
Brand: Abcam Limited
SKU: AB228729
Price: 420 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal HKDC1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Hexokinase HKDC1.

View Alternative Names

Hexokinase HKDC1, Hexokinase domain-containing protein 1, HKDC1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-HKDC1 antibody (AB228729)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HKDC1 antibody (AB228729)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HKDC1 (green) using ab228729 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

Nuclear counterstain : Hoechst 33342 (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HKDC1 antibody (AB228729)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HKDC1 antibody (AB228729)

Paraffin-embedded mouse duodenum tissue stained for HKDC1 using ab228729 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-HKDC1 antibody (AB228729)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-HKDC1 antibody (ab228729) at 1/2000 dilution

All lanes:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Predicted band size: 103 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Hexokinase HKDC1. The exact immunogen used to generate this antibody is proprietary information.

Q2TB90

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HKDC1 protein also known as Hexokinase Domain Containing 1 participates in glucose metabolism by phosphorylating glucose to produce glucose-6-phosphate. It has a molecular mass of approximately 102 kDa. This protein is ubiquitously expressed with higher levels in the liver pancreas and certain fetal tissues. HKDC1 plays an important role in maintaining glucose homeostasis particularly under conditions that require enhanced glucose utilization.

Biological function summary

Hexokinase Domain Containing 1 functions as part of the hexokinase enzyme family contributing to the first step of glycolysis. It might not form a complex but shares similar activity with other hexokinase family members supporting cellular energy supply. The presence of HKDC1 in various tissues suggests its involvement in both normal and stress-related metabolic regulation adapting the cell’s energy production to different physiological demands.

Pathways

The activity of HKDC1 is integrated into the glycolytic pathway and indirectly affects the insulin signaling pathway. In glycolysis it links to glucokinase and other hexokinase enzymes playing a part in glucose metabolism and energy generation within the cell. Its activity impacts the homeostatic control of glucose concentration suggesting a regulatory role in maintaining the balance between energy input and output in metabolic pathways.

Mutations or altered expression of HKDC1 could be linked to metabolic syndromes such as diabetes and gestational diabetes mellitus. Aberrant HKDC1 function may disturb glucose homeostasis leading to these conditions. Moreover connection with other proteins like GCK which also participates in glucose phosphorylation highlights its potential importance in metabolic diseases indicating that HKDC1 might be a target for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the phosphorylation of hexose to hexose 6-phosphate, although at very low level compared to other hexokinases (PubMed : 30517626). Has low glucose phosphorylating activity compared to other hexokinases (PubMed : 30517626). Involved in glucose homeostasis and hepatic lipid accumulation. Required to maintain whole-body glucose homeostasis during pregnancy; however additional evidences are required to confirm this role (By similarity).

See full target information Hexokinase HKDC1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:8034 PubMed37198225

2023

Liver-specific overexpression of HKDC1 increases hepatocyte size and proliferative capacity.

Applications

Unspecified application

Species

Unspecified reactive species

Carolina M Pusec,Vladimir Ilievski,Adam De Jesus,Zeenat Farooq,Joseph L Zapater,Nadia Sweis,Hagar Ismail,Md Wasim Khan,Hossein Ardehali,Jose Cordoba-Chacon,Brian T Layden

Endocrinology 163: PubMed35435980

2022

Enterocyte HKDC1 Modulates Intestinal Glucose Absorption in Male Mice Fed a High-fat Diet.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph L Zapater,Barton Wicksteed,Brian T Layden

Cancer cell international 20:450 PubMed32943998

2020

HKDC1 promotes the tumorigenesis and glycolysis in lung adenocarcinoma via regulating AMPK/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu Wang,Bowen Shi,Yue Zhao,Qijue Lu,Xiang Fei,Chaojing Lu,Chunguang Li,Hezhong Chen

Frontiers in oncology 9:290 PubMed31058090

2019

PGC1β Regulates Breast Tumor Growth and Metastasis by SREBP1-Mediated HKDC1 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoli Chen,Yang Lv,Ying Sun,Hongyu Zhang,Weiguo Xie,Liyan Zhong,Qi Chen,Min Li,Ling Li,Jia Feng,Athena Yao,Qi Zhang,Xiaodong Huang,Zhendong Yu,Paul Yao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HKDC1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-HKDC1 antibody (AB228729)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HKDC1 antibody (AB228729)

Western blot - Anti-HKDC1 antibody (AB228729)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise