AB209846

Anti-HKDC1 antibody [EPR19835]

RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal HKDC1 antibody. Suitable for IP, WB and reacts with Human, Recombinant fragment - Human samples. Cited in 3 publications.

View Alternative Names

Hexokinase HKDC1, Hexokinase domain-containing protein 1, HKDC1

5 Images

Supplier Data

Western blot - Anti-HKDC1 antibody [EPR19835] (AB209846)

Blocking/dilution buffer and concentration : 5% NFDM/TBST.

The cell lysates were kindly provided by our collaborator Dr. Hui Jiang, NIBS

All lanes:

Western blot - Anti-HKDC1 antibody [EPR19835] (ab209846) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

HKDC1-knockdown HeLa cell lysate at 10 µg

Lane 3:

Hexokinase I-knockdown HeLa cell lysate at 10 µg

Lane 4:

Hexokinase II-knockdown HeLa cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 103 kDa

Observed band size: 102 kDa

true

Exposure time: 3min

Supplier Data

Immunoprecipitation - Anti-HKDC1 antibody [EPR19835] (AB209846)

HKDC1 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab209846 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab209846 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg (Input).

Lane 2 : ab209846 IP in HeLa whole cell lysate (+).

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab209846 in HeLa whole cell lysate (-).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-HKDC1 antibody [EPR19835] (ab209846)

Predicted band size: 103 kDa

Observed band size: 102 kDa

false

Supplier Data

Western blot - Anti-HKDC1 antibody [EPR19835] (AB209846)

Blocking/dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-HKDC1 antibody [EPR19835] (ab209846) at 1/1000 dilution

Lane 1:

HKDC1 recombinant protein (aa16-458) 10 ng

Lane 2:

Hexokinase II recombinant protein (aa16-458) 10 ng

Lane 3:

Hexokinase I recombinant protein (aa16-458) 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 103 kDa

Observed band size: 50 kDa

true

Exposure time: 3min

Supplier Data

Western blot - Anti-HKDC1 antibody [EPR19835] (AB209846)

Blocking/dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-HKDC1 antibody [EPR19835] (ab209846) at 1/1000 dilution

Lane 1:

A549 (human lung carcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

Human fetal kidney lysate at 10 µg

Secondary

Lane 1:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lane 2:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/4000 dilution

Predicted band size: 103 kDa

Observed band size: 102 kDa

true

Exposure time: 3min

Supplier Data

Dot Blot - Anti-HKDC1 antibody [EPR19835] (AB209846)

Dot blot analysis of Hexokinase Type III/HK3 using ab321996 at 1 : 1000 (0.525 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : His-tagged human HK3 fragment
Lane2 : His-tagged human HK2 fragment
Lane3 : His-tagged human HK1 fragment
Lane4 : His-tagged human HKDC1 fragment

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human HK1, HK2 and HKDC1.

All lanes:

Dot Blot - Anti-Hexokinase Type III/HK3 antibody [EPR29196-29] (<a href='/en-us/products/primary-antibodies/hexokinase-type-iii-hk3-antibody-epr29196-29-ab321996'>ab321996</a>) at 1/1000 dilution

Lane 1:

His-tagged human HK3 fragment at 5 ng

Lane 2:

His-tagged human HK2 fragment at 5 ng

Lane 3:

His-tagged human HK1 fragment at 5 ng

Lane 4:

His-tagged human HKDC1 fragment at 5 ng

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19835

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Recombinant fragment - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HKDC1 protein also known as Hexokinase Domain Containing 1 participates in glucose metabolism by phosphorylating glucose to produce glucose-6-phosphate. It has a molecular mass of approximately 102 kDa. This protein is ubiquitously expressed with higher levels in the liver pancreas and certain fetal tissues. HKDC1 plays an important role in maintaining glucose homeostasis particularly under conditions that require enhanced glucose utilization.

Biological function summary

Hexokinase Domain Containing 1 functions as part of the hexokinase enzyme family contributing to the first step of glycolysis. It might not form a complex but shares similar activity with other hexokinase family members supporting cellular energy supply. The presence of HKDC1 in various tissues suggests its involvement in both normal and stress-related metabolic regulation adapting the cell’s energy production to different physiological demands.

Pathways

The activity of HKDC1 is integrated into the glycolytic pathway and indirectly affects the insulin signaling pathway. In glycolysis it links to glucokinase and other hexokinase enzymes playing a part in glucose metabolism and energy generation within the cell. Its activity impacts the homeostatic control of glucose concentration suggesting a regulatory role in maintaining the balance between energy input and output in metabolic pathways.

Mutations or altered expression of HKDC1 could be linked to metabolic syndromes such as diabetes and gestational diabetes mellitus. Aberrant HKDC1 function may disturb glucose homeostasis leading to these conditions. Moreover connection with other proteins like GCK which also participates in glucose phosphorylation highlights its potential importance in metabolic diseases indicating that HKDC1 might be a target for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the phosphorylation of hexose to hexose 6-phosphate, although at very low level compared to other hexokinases (PubMed : 30517626). Has low glucose phosphorylating activity compared to other hexokinases (PubMed : 30517626). Involved in glucose homeostasis and hepatic lipid accumulation. Required to maintain whole-body glucose homeostasis during pregnancy; however additional evidences are required to confirm this role (By similarity).

See full target information Hexokinase HKDC1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 11:449 PubMed41057297

2025

Aging aggravated liver ischemia and reperfusion injury by promoting oxidized mtDNA mediated-macrophage pyroptosis through acetylated MCU-dependent calcium uptake.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Yi Wu,Rui Wang,Qi Zhang,Tao Liu,Jun-Yan Liu,Xue-Song Xu,Jun-Hua Gong

Communications biology 8:615 PubMed40234623

2025

HKDC1 promotes ovarian cancer progression through boosting lipid metabolism and immune escape by stabilizing G6PC/G6PC2.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Wang,Juan Chen,Zhan Wang,Xia Luo,Nayiyuan Wu,Jing Wang

Environmental toxicology 39:1269-1282 PubMed37927237

2023

SNHG1, interacting with SND1, contributes to sorafenib resistance of liver cancer cells by increasing m6A-mediated SLC7A11 expression and promoting aerobic glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Li,Shunle Li,Lin Shen,Junhui Li,Di Zhang,Jinmin Yu,Lanxuan Huang,Na Liu,Hongwei Lu,Meng Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HKDC1 antibody [EPR19835]

Western blot - Anti-HKDC1 antibody [EPR19835] (AB209846)

Immunoprecipitation - Anti-HKDC1 antibody [EPR19835] (AB209846)

Western blot - Anti-HKDC1 antibody [EPR19835] (AB209846)

Western blot - Anti-HKDC1 antibody [EPR19835] (AB209846)

Dot Blot - Anti-HKDC1 antibody [EPR19835] (AB209846)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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