AB228817

Anti-HKDC1 antibody [EPR19835] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal HKDC1 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication.

View Alternative Names

Hexokinase HKDC1, Hexokinase domain-containing protein 1, HKDC1

2 Images

Supplier Data

Immunoprecipitation - Anti-HKDC1 antibody [EPR19835] - BSA and Azide free (AB228817)

HKDC1 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab209846 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab209846 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg (Input).

Lane 2 : ab209846 IP in HeLa whole cell lysate (+).

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab209846 in HeLa whole cell lysate (-).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209846).

All lanes:

Immunoprecipitation - Anti-HKDC1 antibody [EPR19835] (<a href='/en-us/products/primary-antibodies/hkdc1-antibody-epr19835-ab209846'>ab209846</a>)

Predicted band size: 103 kDa

Observed band size: 102 kDa

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Supplier Data

Western blot - Anti-HKDC1 antibody [EPR19835] - BSA and Azide free (AB228817)

Blocking/dilution buffer and concentration : 5% NFDM/TBST.

The cell lysates were kindly provided by our collaborator Dr. Hui Jiang, NIBS.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209846).

All lanes:

Western blot - Anti-HKDC1 antibody [EPR19835] (<a href='/en-us/products/primary-antibodies/hkdc1-antibody-epr19835-ab209846'>ab209846</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

HKDC1-knockdown HeLa cell lysate at 10 µg

Lane 3:

Hexokinase I-knockdown HeLa cell lysate at 10 µg

Lane 4:

Hexokinase II-knockdown HeLa cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 103 kDa

Observed band size: 102 kDa

true

Exposure time: 3min

Unconjugated

Anti-HKDC1 antibody [EPR19835]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19835

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab228817 is the carrier-free version of ab209846.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HKDC1 protein also known as Hexokinase Domain Containing 1 participates in glucose metabolism by phosphorylating glucose to produce glucose-6-phosphate. It has a molecular mass of approximately 102 kDa. This protein is ubiquitously expressed with higher levels in the liver pancreas and certain fetal tissues. HKDC1 plays an important role in maintaining glucose homeostasis particularly under conditions that require enhanced glucose utilization.

Biological function summary

Hexokinase Domain Containing 1 functions as part of the hexokinase enzyme family contributing to the first step of glycolysis. It might not form a complex but shares similar activity with other hexokinase family members supporting cellular energy supply. The presence of HKDC1 in various tissues suggests its involvement in both normal and stress-related metabolic regulation adapting the cell’s energy production to different physiological demands.

Pathways

The activity of HKDC1 is integrated into the glycolytic pathway and indirectly affects the insulin signaling pathway. In glycolysis it links to glucokinase and other hexokinase enzymes playing a part in glucose metabolism and energy generation within the cell. Its activity impacts the homeostatic control of glucose concentration suggesting a regulatory role in maintaining the balance between energy input and output in metabolic pathways.

Mutations or altered expression of HKDC1 could be linked to metabolic syndromes such as diabetes and gestational diabetes mellitus. Aberrant HKDC1 function may disturb glucose homeostasis leading to these conditions. Moreover connection with other proteins like GCK which also participates in glucose phosphorylation highlights its potential importance in metabolic diseases indicating that HKDC1 might be a target for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the phosphorylation of hexose to hexose 6-phosphate, although at very low level compared to other hexokinases (PubMed : 30517626). Has low glucose phosphorylating activity compared to other hexokinases (PubMed : 30517626). Involved in glucose homeostasis and hepatic lipid accumulation. Required to maintain whole-body glucose homeostasis during pregnancy; however additional evidences are required to confirm this role (By similarity).

See full target information Hexokinase HKDC1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cells 12: PubMed37626835

2023

Neuronal Coordinates Autophagy Induction and Physiological Adaptations to Balance mTORC1 Signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Athanasios Metaxakis,Michail Pavlidis,Nektarios Tavernarakis

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HKDC1 antibody [EPR19835] - BSA and Azide free

Immunoprecipitation - Anti-HKDC1 antibody [EPR19835] - BSA and Azide free (AB228817)

Western blot - Anti-HKDC1 antibody [EPR19835] - BSA and Azide free (AB228817)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise