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AB24093

Anti-HL60 antibody [IPO-M6]

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(1 Publication)

Mouse Monoclonal HL-60 antibody. Suitable for IHC-Fr, Flow Cyt, IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing HL60 protein.

View Alternative Names

human leukemia cell line HL60

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

IPO-M6

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, IHC-Fr, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Reacts with human leukemia cell line HL60 and immuno-precipitates with two proteins with MW of 48 and 52 kDa. ab24093 does not stain B cell lines Daudy, PHS, Namalwa, RPMI-1788 and T-cell lines CCRF-HSB2, Jurkat and Molt-4.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" } } }
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Product details

This antibody can be applied for staining of monocytes and up to 10 % of lymphocytes from peripheral blood of healthy donors. Blast cells of patients with AMMonL (M5 following FAB classification), AMMonL (M4) and hairy cells leukemia were ab24093 positive. The antigen, defined with this mAb, is particularly expressed on blood cells from patients with infectious mononucleosis and CLL. Histiocytes and macrophages were also positive. Malignant cells from patients with AML (M1 and M2), T-ALL, B-ALL were ab24093 negative.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.3 Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HL60 cell line named for its original derivation from human promyelocytic leukemia serves as a model for studying various aspects of blood cell biology and cancer research. HL60 cells are suspension cells and feature the unique ability to differentiate into several cell types such as granulocytes and macrophage-like cells depending on the stimuli. The cells express markers for early hematopoietic progenitors as well as proteins relevant to immune response. Mass-specific information for the HL60 line is not applicable as it refers to a cell line rather than a solitary protein or molecule.
Biological function summary

HL60 cells provide insights into modulations within the hematopoietic lineage. Scientists frequently use it to study the process of myeloid differentiation offering a stable platform for experiments involving gene expression and genetic modifications. The cell line is not part of a specific protein complex but plays an essential role when studying signal transduction pathways affecting myeloid cell differentiation. Researchers widely apply these cells to analyze transcription factors and cytokine interactions.

Pathways

The HL60 cell line facilitates exploration of the mitogen-activated protein kinase (MAPK) pathway and the role it plays in cell proliferation and differentiation. This pathway interacts with key proteins such as extracellular signal-regulated kinases (ERK) and c-Jun N-terminal kinases (JNK) influencing cellular outcomes by receiving and interpreting a multitude of metabolic and external signals. HL60 serves as a powerful tool for dissecting molecular regulation and signal integration within these pathways.

The HL60 cell line contributes significantly to the understanding of acute promyelocytic leukemia given its original derivation from this cancer type. Researchers employ these cells to test therapeutic interventions and understand drug resistance mechanisms related to leukemia. Moreover leading-edge studies utilize HL60 cells to explore the effects of new drugs targeting the modifications of the proteins involved in the disease such as PML-RARα the oncogenic driver in promyelocytic leukemia.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information HL-60
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Pediatric research 79:614-20 PubMed26672737

2015

Antenatal retinoic acid administration increases trophoblastic retinol-binding protein dependent retinol transport in the nitrofen model of congenital diaphragmatic hernia.

Applications

Unspecified application

Species

Unspecified reactive species

Balazs Kutasy,Florian Friedmacher,Lara Pes,David Coyle,Takashi Doi,Francesca Paradisi,Prem Puri
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