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Rabbit Recombinant Monoclonal HLAC antibody. Carrier free. Suitable for IP, IHC-P, WB and reacts with Human, Transfected cell lysate - Human samples.

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Images

Western blot - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (AB307362), expandable thumbnail
  • Western blot - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (AB307362), expandable thumbnail
  • Western blot - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (AB307362), expandable thumbnail
  • Western blot - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (AB307362), expandable thumbnail
  • Immunoprecipitation - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (AB307362), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPIHC-PWBFlow CytICC/IF
Human
Tested
Tested
Tested
Not recommended
Not recommended
Transfected cell lysate - Human
Not recommended
Not recommended
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Transfected cell lysate - Human
Dilution info
-
Notes

-

Target data

Function

Antigen-presenting major histocompatibility complex class I (MHCI) molecule with an important role in reproduction and antiviral immunity (PubMed:11172028, PubMed:20104487, PubMed:20439706, PubMed:20972337, PubMed:24091323, PubMed:28649982, PubMed:29312307). In complex with B2M/beta 2 microglobulin displays a restricted repertoire of self and viral peptides and acts as a dominant ligand for inhibitory and activating killer immunoglobulin receptors (KIRs) expressed on NK cells (PubMed:16141329). In an allogeneic setting, such as during pregnancy, mediates interaction of extravillous trophoblasts with KIR on uterine NK cells and regulate trophoblast invasion necessary for placentation and overall fetal growth (PubMed:20972337, PubMed:24091323). During viral infection, may present viral peptides with low affinity for KIRs, impeding KIR-mediated inhibition through peptide antagonism and favoring lysis of infected cells (PubMed:20439706). Presents a restricted repertoire of viral peptides on antigen-presenting cells for recognition by alpha-beta T cell receptor (TCR) on HLA-C-restricted CD8-positive T cells, guiding antigen-specific T cell immune response to eliminate infected cells, particularly in chronic viral infection settings such as HIV-1 or CMV infection (PubMed:11172028, PubMed:20104487, PubMed:28649982). Both the peptide and the MHC molecule are recognized by TCR, the peptide is responsible for the fine specificity of antigen recognition and MHC residues account for the MHC restriction of T cells (By similarity). Typically presents intracellular peptide antigens of 9 amino acids that arise from cytosolic proteolysis via proteasome. Can bind different peptides containing allele-specific binding motifs, which are mainly defined by anchor residues at position 2 and 9. Preferentially displays peptides having a restricted repertoire of hydrophobic or aromatic amino acids (Phe, Ile, Leu, Met, Val and Tyr) at the C-terminal anchor (PubMed:25311805, PubMed:8265661). ALLELE C*01:02: The peptide-bound form interacts with KIR2DL2 and KIR2DL3 inhibitory receptors on NK cells. The low affinity peptides compete with the high affinity peptides impeding KIR-mediated inhibition and favoring lysis of infected cells (PubMed:20439706). Presents to CD8-positive T cells a CMV epitope derived from UL83/pp65 (RCPEMISVL), an immediate-early antigen necessary for initiating viral replication (PubMed:12947002). ALLELE C*04:01: Presents a conserved HIV-1 epitope derived from env (SFNCGGEFF) to memory CD8-positive T cells, eliciting very strong IFNG responses (PubMed:20104487). Presents CMV epitope derived from UL83/pp65 (QYDPVAALF) to CD8-positive T cells, triggering T cell cytotoxic response (PubMed:12947002). ALLELE C*05:01: Presents HIV-1 epitope derived from rev (SAEPVPLQL) to CD8-positive T cells, triggering T cell cytotoxic response. ALLELE C*06:02: In trophoblasts, interacts with KIR2DS2 on uterine NK cells and triggers NK cell activation, including secretion of cytokines such as GMCSF that enhances trophoblast migration. ALLELE C*07:02: Plays an important role in the control of chronic CMV infection. Presents immunodominant CMV epitopes derived from IE1 (LSEFCRVL and CRVLCCYVL) and UL28 (FRCPRRFCF), both antigens synthesized during immediate-early period of viral replication. Elicits a strong anti-viral CD8-positive T cell immune response that increases markedly with age. ALLELE C*08:01: Presents viral epitopes derived from CMV UL83 (VVCAHELVC) and IAV M1 (GILGFVFTL), triggering CD8-positive T cell cytotoxic response. ALLELE C*12:02: Presents CMV epitope derived from UL83 (VAFTSHEHF) to CD8-positive T cells. ALLELE C*15:02: Presents CMV epitope derived from UL83 CC (VVCAHELVC) to CD8-positive T cells, triggering T cell cytotoxic response.

Alternative names

HLAC, HLA-C, HLA-Cw, Human leukocyte antigen C

Recommended products

Rabbit Recombinant Monoclonal HLAC antibody. Carrier free. Suitable for IP, IHC-P, WB and reacts with Human, Transfected cell lysate - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR26121-73
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362), expandable thumbnail

    Western blot - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)

    This data was developed using Anti-HLA-C antibody [EPR26121-73] ab307361, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    This antibody does not cross-react with human HLA-A and HLA-B.
    Exposure time: 3.25 seconds

    All lanes: Western blot - Anti-HLA-C antibody [EPR26121-73] (Anti-HLA-C antibody [EPR26121-73] ab307361) at 1/1000 dilution

    Lane 1: HEK-293T cells transfected with an empty vector containi a his tag whole cell lysate at 20 µg

    Lane 2: HEK-293T cells transfected with a human HLA-C expression vector containi a his tag whole cell lysate at 20 µg

    Lane 3: HEK-293T cells transfected with a human HLA-A expression vector containi a his tag whole cell lysate at 20 µg

    Lane 4: HEK-293T cells transfected with a human HLA-B expression vector containi a his tag whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 41 kDa

    Exposure time: 3.25s

  • Western blot - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362), expandable thumbnail

    Western blot - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)

    This data was developed using Anti-HLA-C antibody [EPR26121-73] ab307361, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 7.25 seconds

    All lanes: Western blot - Anti-HLA-C antibody [EPR26121-73] (Anti-HLA-C antibody [EPR26121-73] ab307361) at 1/1000 dilution

    All lanes: Human colon tissue lysate at 40 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 41 kDa

    Exposure time: 7.25s

  • Western blot - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362), expandable thumbnail

    Western blot - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)

    This data was developed using Anti-HLA-C antibody [EPR26121-73] ab307361, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 15 seconds

    All lanes: Western blot - Anti-HLA-C antibody [EPR26121-73] (Anti-HLA-C antibody [EPR26121-73] ab307361) at 1/1000 dilution

    Lane 1: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 80 µg

    Lane 2: A375 (human malignant melanoma epithelial cell) whole cell lysate at 80 µg

    Lane 3: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 80 µg

    Lane 4: Human lymphoma tissue lysate at 80 µg

    Lane 5: HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate at 80 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 41 kDa

    Exposure time: 15s

  • Western blot - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362), expandable thumbnail

    Western blot - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)

    This data was developed using Anti-HLA-C antibody [EPR26121-73] ab307361, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 26 seconds

    All lanes: Western blot - Anti-HLA-C antibody [EPR26121-73] (Anti-HLA-C antibody [EPR26121-73] ab307361) at 1/1000 dilution

    Lane 1: Jurkat (human T cell leukemia T lymphocyte) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: Jurkat transfected with siRNA specifically targeti HLA-C whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 41 kDa

    Exposure time: 26s

  • Immunoprecipitation - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362), expandable thumbnail

    Immunoprecipitation - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)

    This data was developed using Anti-HLA-C antibody [EPR26121-73] ab307361, the same antibody clone in a different buffer formulation.
    HLA-C was immunoprecipitated from 0.35 mg Jurkat whole cell lysate with Anti-HLA-C antibody [EPR26121-73] ab307361 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-HLA-C antibody [EPR26121-73] ab307361 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
    Lane 2: Anti-HLA-C antibody [EPR26121-73] ab307361 IP in Jurkat whole cell lysate
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-HLA-C antibody [EPR26121-73] ab307361 in Jurkat whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 10 seconds

    All lanes: Immunoprecipitation - Anti-HLA-C antibody [EPR26121-73] (Anti-HLA-C antibody [EPR26121-73] ab307361) at 1/30 dilution

    All lanes: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 10s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)

    This data was developed using Anti-HLA-C antibody [EPR26121-73] ab307361, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling HLA-C with Anti-HLA-C antibody [EPR26121-73] ab307361 at 1/4000 (0.126 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human colon (PMID: 17008885). The section was incubated with Anti-HLA-C antibody [EPR26121-73] ab307361 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 10 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)

    This data was developed using Anti-HLA-C antibody [EPR26121-73] ab307361, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human NKT lymphoma tissue labeling HLA-C with Anti-HLA-C antibody [EPR26121-73] ab307361 at 1/4000 (0.126 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on NKT lymphoma. The section was incubated with Anti-HLA-C antibody [EPR26121-73] ab307361 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 10 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (ab307362)

    This data was developed using Anti-HLA-C antibody [EPR26121-73] ab307361, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling HLA-C with Anti-HLA-C antibody [EPR26121-73] ab307361 at 1/4000 (0.126 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human tonsil (PMID: 17008885). The section was incubated with Anti-HLA-C antibody [EPR26121-73] ab307361 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 10 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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