Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (AB307362)

This data was developed using ab307361, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling HLA-C with ab307361 at 1/4000 (0.126 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human tonsil (PMID : 17008885). The section was incubated with ab307361 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 10 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (AB307362)

This data was developed using ab307361, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human NKT lymphoma tissue labeling HLA-C with ab307361 at 1/4000 (0.126 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on NKT lymphoma. The section was incubated with ab307361 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 10 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (AB307362)

This data was developed using ab307361, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling HLA-C with ab307361 at 1/4000 (0.126 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human colon (PMID : 17008885). The section was incubated with ab307361 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 10 mins

• IP

Supplier Data

Immunoprecipitation - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (AB307362)

This data was developed using ab307361, the same antibody clone in a different buffer formulation. HLA-C was immunoprecipitated from 0.35 mg Jurkat whole cell lysate with ab307361 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307361 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate Lane 2 : ab307361 IP in Jurkat whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab307361 in Jurkat whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 10 seconds

All lanes:

Immunoprecipitation - Anti-HLA-C antibody [EPR26121-73] (<a href='/en-us/products/primary-antibodies/hla-c-antibody-epr26121-73-ab307361'>ab307361</a>) at 1/30 dilution

All lanes:

Jurkat (human T cell leukemia T lymphocyte) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (AB307362)

This data was developed using ab307361, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 26 seconds

All lanes:

Western blot - Anti-HLA-C antibody [EPR26121-73] (<a href='/en-us/products/primary-antibodies/hla-c-antibody-epr26121-73-ab307361'>ab307361</a>) at 1/1000 dilution

Lane 1:

Jurkat (human T cell leukemia T lymphocyte) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

Jurkat transfected with siRNA specifically targeti HLA-C whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 41 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (AB307362)

This data was developed using ab307361, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 7.25 seconds

All lanes:

Western blot - Anti-HLA-C antibody [EPR26121-73] (<a href='/en-us/products/primary-antibodies/hla-c-antibody-epr26121-73-ab307361'>ab307361</a>) at 1/1000 dilution

All lanes:

Human colon tissue lysate at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 41 kDa

false

Exposure time: 7.25s

• WB

Supplier Data

Western blot - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (AB307362)

This data was developed using ab307361, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 15 seconds

All lanes:

Western blot - Anti-HLA-C antibody [EPR26121-73] (<a href='/en-us/products/primary-antibodies/hla-c-antibody-epr26121-73-ab307361'>ab307361</a>) at 1/1000 dilution

Lane 1:

Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 80 µg

Lane 2:

A375 (human malignant melanoma epithelial cell) whole cell lysate at 80 µg

Lane 3:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 80 µg

Lane 4:

Human lymphoma tissue lysate at 80 µg

Lane 5:

HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 41 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-HLA-C antibody [EPR26121-73] - BSA and Azide free (AB307362)

This data was developed using ab307361, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody does not cross-react with human HLA-A and HLA-B. Exposure time : 3.25 seconds

All lanes:

Western blot - Anti-HLA-C antibody [EPR26121-73] (<a href='/en-us/products/primary-antibodies/hla-c-antibody-epr26121-73-ab307361'>ab307361</a>) at 1/1000 dilution

Lane 1:

HEK-293T cells transfected with an empty vector containi a his tag whole cell lysate at 20 µg

Lane 2:

HEK-293T cells transfected with a human HLA-C expression vector containi a his tag whole cell lysate at 20 µg

Lane 3:

HEK-293T cells transfected with a human HLA-A expression vector containi a his tag whole cell lysate at 20 µg

Lane 4:

HEK-293T cells transfected with a human HLA-B expression vector containi a his tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 41 kDa

false

Exposure time: 3.25s