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AB248588

Anti-HLA DMB antibody [EPR7981] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal HLA DMB antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Transfected cell line - Human, Human samples. Cited in 1 publication.

View Alternative Names

DMB, RING7, HLA-DMB, MHC class II antigen DMB, Really interesting new gene 7 protein

4 Images
Flow Cytometry (Intracellular) - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (AB248588)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (AB248588)

This data was developed using ab133640, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of permeabilized Raji cells labelling HLA DMB with ab133640 at 1/100 dilution (red). Rabbit IgG (negative control) shown in green.

Western blot - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (AB248588)
  • WB

Unknown

Western blot - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (AB248588)

This data was developed using ab133640, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HLA DMB antibody [EPR7981] (<a href='/en-us/products/primary-antibodies/hla-dmb-antibody-epr7981-ab133640'>ab133640</a>) at 1/1000 dilution

Lane 1:

Raji lysate at 10 µg

Lane 2:

Daudi lysate at 10 µg

Lane 3:

Human spleen lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 29 kDa

false

Western blot - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (AB248588)
  • WB

Lab

Western blot - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (AB248588)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. ab181602 was used as a loading control. This data was developed using ab133640, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HLA DMB antibody [EPR7981] (<a href='/en-us/products/primary-antibodies/hla-dmb-antibody-epr7981-ab133640'>ab133640</a>) at 1/10000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) whole cell lysate with blank vector transfected at 15 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) whole cell lysate with His tagged HLA DMB expression vector transfected at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Exposure time: 1s

OI-RD Scanning - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (AB248588)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (AB248588)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7981

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Transfected cell line - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Product details

ab248588 is the carrier-free version of ab133640.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a critical role in catalyzing the release of class II-associated invariant chain peptide (CLIP) from newly synthesized MHC class II molecules and freeing the peptide binding site for acquisition of antigenic peptides. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO.
See full target information HLA-DMB
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Immunity, inflammation and disease 10:e727 PubMed36301031

2022

Forkhead box M1 recruits FoxP3 Treg cells to induce immune escape in hilar cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Ma,Zhaowei Sun,Xueliang Li,Jingyun Guo,Qinlei Wang,Mujian Teng
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com