Rabbit Recombinant Monoclonal HLA DMB antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Transfected cell line - Human, Human samples.
Images
![Western blot - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (AB248588), expandable thumbnail](https://content.abcam.com/products/images/hla-dmb-antibody-epr7981-bsa-and-azide-free-ab248588--western-blot-img131271.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (AB248588)")
![Flow Cytometry (Intracellular) - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (AB248588), expandable thumbnail](https://content.abcam.com/products/images/hla-dmb-antibody-epr7981-bsa-and-azide-free-ab248588--flow-cytometry-intracellular-img27260.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (AB248588)")
![OI-RD Scanning - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (AB248588), expandable thumbnail](https://content.abcam.com/products/images/hla-dmb-antibody-epr7981-bsa-and-azide-free-ab248588--oi-rd-scanning-img85200.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (AB248588)")
![Western blot - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (AB248588), expandable thumbnail](https://content.abcam.com/products/images/hla-dmb-antibody-epr7981-bsa-and-azide-free-ab248588--western-blot-img416089.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (AB248588)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | Flow Cyt (Intra) | IHC-P | IP | |
|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended |
| Transfected cell line - Human | Tested | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell line - Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell line - Human | Dilution info - | Notes - |
Target data
Function
Plays a critical role in catalyzing the release of class II-associated invariant chain peptide (CLIP) from newly synthesized MHC class II molecules and freeing the peptide binding site for acquisition of antigenic peptides. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO.
Alternative names
DMB, RING7, HLA-DMB, MHC class II antigen DMB, Really interesting new gene 7 protein
Recommended products
Rabbit Recombinant Monoclonal HLA DMB antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Transfected cell line - Human, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR7981
- Purification technique
- Affinity purification
- Dissociation constant
- 7.4 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248588 is the carrier-free version of Anti-HLA DMB antibody [EPR7981] ab133640.
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (ab248588), expandable thumbnail](https://content.abcam.com/products/images/hla-dmb-antibody-epr7981-bsa-and-azide-free-ab248588--western-blot-img131271.jpg "Western blot - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (ab248588)")
Western blot - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (ab248588)
This data was developed using Anti-HLA DMB antibody [EPR7981] ab133640, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-HLA DMB antibody [EPR7981] (Anti-HLA DMB antibody [EPR7981] ab133640) at 1/1000 dilution
Lane 1: Raji lysate at 10 µg
Lane 2: Daudi lysate at 10 µg
Lane 3: Human spleen lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 29 kDa
![Flow Cytometry (Intracellular) - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (ab248588), expandable thumbnail](https://content.abcam.com/products/images/hla-dmb-antibody-epr7981-bsa-and-azide-free-ab248588--flow-cytometry-intracellular-img27260.jpg "Flow Cytometry (Intracellular) - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (ab248588)")
Flow Cytometry (Intracellular) - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (ab248588)
This data was developed using Anti-HLA DMB antibody [EPR7981] ab133640, the same antibody clone in a different buffer formulation.
Intracellular Flow Cytometry analysis of permeabilized Raji cells labelling HLA DMB with Anti-HLA DMB antibody [EPR7981] ab133640 at 1/100 dilution (red). Rabbit IgG (negative control) shown in green.
![OI-RD Scanning - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (ab248588), expandable thumbnail](https://content.abcam.com/products/images/hla-dmb-antibody-epr7981-bsa-and-azide-free-ab248588--oi-rd-scanning-img85200.jpg "OI-RD Scanning - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (ab248588)")
OI-RD Scanning - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (ab248588)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.![Western blot - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (ab248588), expandable thumbnail](https://content.abcam.com/products/images/hla-dmb-antibody-epr7981-bsa-and-azide-free-ab248588--western-blot-img416089.jpg "Western blot - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (ab248588)")
Western blot - Anti-HLA DMB antibody [EPR7981] - BSA and Azide free (ab248588)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a loading control.
This data was developed using Anti-HLA DMB antibody [EPR7981] ab133640, the same antibody clone in a different buffer formulation.All lanes: Western blot - Anti-HLA DMB antibody [EPR7981] (Anti-HLA DMB antibody [EPR7981] ab133640) at 1/10000 dilution
Lane 1: 293T (Human embryonic kidney epithelial cell) whole cell lysate with blank vector transfected at 15 µg
Lane 2: 293T (Human embryonic kidney epithelial cell) whole cell lysate with His tagged HLA DMB expression vector transfected at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 1s
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