JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB131273

Anti-HLA DMB antibody [EPR7982]

Be the first to review this product! Submit a review

|

(4 Publications)

Rabbit Recombinant Monoclonal HLA DMB antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Transfected cell line - Human, Human samples. Cited in 4 publications.

View Alternative Names

DMB, RING7, HLA-DMB, MHC class II antigen DMB, Really interesting new gene 7 protein

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DMB antibody [EPR7982] (AB131273)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DMB antibody [EPR7982] (AB131273)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling HLA DMB with ab131273 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-HLA DMB antibody [EPR7982] (AB131273)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HLA DMB antibody [EPR7982] (AB131273)

Immunofluorescence analysis of Raji cells labelling HLA DMB with ab131273 at 1/250 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DMB antibody [EPR7982] (AB131273)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DMB antibody [EPR7982] (AB131273)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling HLA DMB with ab131273 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-HLA DMB antibody [EPR7982] (AB131273)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-HLA DMB antibody [EPR7982] (AB131273)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells labelling HLA DMB with primary antibody anti-HLA DMB (ab131273) at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080) secondary antibody at 1/500 dilution. Confocal image showing cytoplasmic staining in 293T cells transfected with myc-tagged HLA-DMB expression vector, no staining in 293T cells transfected with myc-tagged NEK6 expression vector. Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 488) (ab202008) was used as counterstain at 1/100 dilution. The nuclear counter stain is DAPI (blue).

Western blot - Anti-HLA DMB antibody [EPR7982] (AB131273)
  • WB

Unknown

Western blot - Anti-HLA DMB antibody [EPR7982] (AB131273)

All lanes:

Western blot - Anti-HLA DMB antibody [EPR7982] (ab131273) at 1/1000 dilution

Lane 1:

Raji cell lysate at 10 µg

Lane 2:

Daudi cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 29 kDa

false

Western blot - Anti-HLA DMB antibody [EPR7982] (AB131273)
  • WB

Lab

Western blot - Anti-HLA DMB antibody [EPR7982] (AB131273)

Blocking buffer and concentration : 5% NFDM/TBST Diluting buffer and concentration : 5% NFDM/TBST This antibody does not cross-react with human NEK6.

Lanes 1 - 2:

Western blot - Anti-HLA DMB antibody [EPR7982] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/hla-dmb-antibody-epr7982-bsa-and-azide-free-ab248398'>ab248398</a>) at 1/1000 dilution

Lanes 1 - 2:

Western blot - Anti-HLA DMB antibody [EPR7982] (ab131273) at 1/1000 dilution

Lane 1:

HEK-293T cells transfected with a human HLA-DMB expression vector containing a his tag, whole cell lysate at 20 µg

Lane 2:

HEK-293T cells transfected with a human NEK6 expression vector containing a his tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Exposure time: 26s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7982

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" }, "Transfected cell line - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
style
left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Plays a critical role in catalyzing the release of class II-associated invariant chain peptide (CLIP) from newly synthesized MHC class II molecules and freeing the peptide binding site for acquisition of antigenic peptides. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO.
See full target information HLA-DMB
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 21:e13707 PubMed36087066

2022

The lysosomal proteome of senescent cells contributes to the senescence secretome.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel Rovira,Rebecca Sereda,David Pladevall-Morera,Valentina Ramponi,Ines Marin,Mate Maus,Julio Madrigal-Matute,Antonio Díaz,Fernando García,Javier Muñoz,Ana María Cuervo,Manuel Serrano

Oncoimmunology 11:2080329 PubMed35655709

2022

MHC class II molecules on pancreatic cancer cells indicate a potential for neo-antigen-based immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Renato B Baleeiro,Christian J Bouwens,Peng Liu,Carmela Di Gioia,Louisa S Chard Dunmall,Ai Nagano,Rathistevy Gangeswaran,Claude Chelala,Hemant M Kocher,Nicholas R Lemoine,Yaohe Wang

Human immunology 79:558-563 PubMed29656111

2018

Circulating histocompatibility antigen (HLA) gene products may help differentiate benign from malignant indeterminate pulmonary lesions.

Applications

WB

Species

Unspecified reactive species

Smriti Kanangat,Christopher W Seder,Melissa R Pergande,Gabriela C Lobato,Cristina L Fhied,Maryam F Raouf,Michael J Liptay,Jeffrey A Borgia

Scientific reports 7:14416 PubMed29089548

2017

HLA-DMB restricts human T-cell leukemia virus type-1 (HTLV-1) protein expression via regulation of ATG7 acetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Wang,Di Song,Yanzi Liu,Guangjian Lu,Shuai Yang,Lu Liu,Zhitao Gao,Lingling Ma,Zhixiang Guo,Chenguang Zhang,Hui Wang,Bo Yang
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB133640

Anti-HLA DMB antibody [EPR7981]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-HLA DMB antibody [EPR7981] (AB133640)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB133578

Anti-HLA Class II DRB1 antibody [EPR6148]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [EPR6148] (AB133578)

  • 5
  • 1
Primary Antibodies

AB22432

Anti-HLA Class I antibody [W6/32]

Flow Cytometry - Anti-HLA Class I antibody [W6/32] (AB22432)

  • 5
  • 1
Primary Antibodies

AB225636

Anti-HLA Class 1 ABC antibody [EPR22172]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Flow Cytometry - Anti-HLA Class 1 ABC antibody [EPR22172] (AB225636)

  • 4
  • 1
Primary Antibodies

AB226777

Anti-HLA DRB5 antibody

Western blot - Anti-HLA DRB5 antibody (AB226777)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com