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AB248398

Anti-HLA DMB antibody [EPR7982] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal HLA DMB antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Transfected cell line - Human, Human samples. Cited in 1 publication.

View Alternative Names

DMB, RING7, HLA-DMB, MHC class II antigen DMB, Really interesting new gene 7 protein

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DMB antibody [EPR7982] - BSA and Azide free (AB248398)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DMB antibody [EPR7982] - BSA and Azide free (AB248398)

This data was developed using ab131273, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling HLA DMB with ab131273 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-HLA DMB antibody [EPR7982] - BSA and Azide free (AB248398)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HLA DMB antibody [EPR7982] - BSA and Azide free (AB248398)

This data was developed using ab131273, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of Raji cells labelling HLA DMB with ab131273 at 1/250 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DMB antibody [EPR7982] - BSA and Azide free (AB248398)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DMB antibody [EPR7982] - BSA and Azide free (AB248398)

This data was developed using ab131273, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling HLA DMB with ab131273 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-HLA DMB antibody [EPR7982] - BSA and Azide free (AB248398)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-HLA DMB antibody [EPR7982] - BSA and Azide free (AB248398)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells labelling HLA DMB with primary antibody anti-HLA DMB (ab131273) at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080) secondary antibody at 1/500 dilution. Confocal image showing cytoplasmic staining in 293T cells transfected with myc-tagged HLA-DMB expression vector, no staining in 293T cells transfected with myc-tagged NEK6 expression vector. Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 488) (ab202008) was used as counterstain at 1/100 dilution. The nuclear counter stain is DAPI (blue). This data was developed using ab131273, the same antibody clone in a different buffer formulation.

Western blot - Anti-HLA DMB antibody [EPR7982] - BSA and Azide free (AB248398)
  • WB

Unknown

Western blot - Anti-HLA DMB antibody [EPR7982] - BSA and Azide free (AB248398)

This data was developed using ab131273, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HLA DMB antibody [EPR7982] (<a href='/en-us/products/primary-antibodies/hla-dmb-antibody-epr7982-ab131273'>ab131273</a>) at 1/1000 dilution

Lane 1:

Raji cell lysate at 10 µg

Lane 2:

Daudi cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 29 kDa

false

Western blot - Anti-HLA DMB antibody [EPR7982] - BSA and Azide free (AB248398)
  • WB

Lab

Western blot - Anti-HLA DMB antibody [EPR7982] - BSA and Azide free (AB248398)

Blocking buffer and concentration : 5% NFDM/TBST Diluting buffer and concentration : 5% NFDM/TBST This antibody does not cross-react with human NEK6. This data was developed using ab131273, the same antibody clone in a different buffer formulation.

Lanes 1 - 2:

Western blot - Anti-HLA DMB antibody [EPR7982] - BSA and Azide free (ab248398) at 1/1000 dilution

Lanes 1 - 2:

Western blot - Anti-HLA DMB antibody [EPR7982] (<a href='/en-us/products/primary-antibodies/hla-dmb-antibody-epr7982-ab131273'>ab131273</a>) at 1/1000 dilution

Lane 1:

HEK-293T cells transfected with a human HLA-DMB expression vector containing a his tag, whole cell lysate at 20 µg

Lane 2:

HEK-293T cells transfected with a human NEK6 expression vector containing a his tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Exposure time: 26s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7982

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" }, "Transfected cell line - Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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Product details

ab248398 is the carrier-free version of ab131273.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a critical role in catalyzing the release of class II-associated invariant chain peptide (CLIP) from newly synthesized MHC class II molecules and freeing the peptide binding site for acquisition of antigenic peptides. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO.
See full target information HLA-DMB
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 24:457 PubMed36380881

2022

Suppressive effect of YY1-mediated RGS22 regulation on the proliferation, migration and invasion of pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shou-Ji Cao,Wan-Li Ge,Ling-Dong Meng,Qun Chen,Yi Miao,Kui-Rong Jiang,Jing-Jing Zhang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com