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AB157210

Anti-HLA-DPB1 antibody [EPR11226]

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(1 Review)

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(23 Publications)

Anti-HLA-DPB1 antibody [EPR11226] (ab157210) is a rabbit monoclonal antibody detecting HLA-DPB1 in Western Blot, IP, IHC-P, ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications

View Alternative Names

HLA-DP1B, HLA-DPB1, MHC class II antigen DPB1

11 Images
Immunocytochemistry/ Immunofluorescence - Anti-HLA-DPB1 antibody [EPR11226] (AB157210)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-HLA-DPB1 antibody [EPR11226] (AB157210)

ab157210 staining HLA-DPB1 in HDLM-2 (human Hodgkin lymphoma cell) cells at 1/1000 dilution (1.26 μg/ml). The cells were fixed with 80% Methanol, and permeabilized with 0.1% PBS-Triton X-100. Positive staining in HDLM-2 cell line (shown in green) and negative staining in HEK-293 cell line. The counterstain, ab195889 Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker at 1/200 (2.5 μg/ml) was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope.

Negative control : HEK-293

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR11226] (AB157210)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR11226] (AB157210)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MHC Class II with unpurified ab157210 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR11226] (AB157210)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR11226] (AB157210)

Unpurified ab157210 showing positiveve staining in human normal colon.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR11226] (AB157210)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR11226] (AB157210)

Unpurified ab157210 showing negative staining in Human normal brain.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR11226] (AB157210)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR11226] (AB157210)

Immunohistochemical staining of paraffin embedded human tonsil with purified ab157210 at a working dilution of 1/2500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR11226] (AB157210)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR11226] (AB157210)

Unpurified ab157210 showing negative staining in Human heart.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR11226] (AB157210)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR11226] (AB157210)

Immunohistochemical staining of paraffin embedded human skeletal muscle with purified ab157210 at a working dilution of 1/2500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Immunoprecipitation - Anti-HLA-DPB1 antibody [EPR11226] (AB157210)
  • IP

Unknown

Immunoprecipitation - Anti-HLA-DPB1 antibody [EPR11226] (AB157210)

ab157210 (purified) at 1/70 immunoprecipitating MHC Class II in 10 μg Daudi cell lysate (Lanes 1 and 2, observed at 29 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution. Blocking buffer and concentration : 5% NFDM/TBST Dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-HLA-DPB1 antibody [EPR11226] (ab157210)

Predicted band size: 29 kDa

false

Western blot - Anti-HLA-DPB1 antibody [EPR11226] (AB157210)
  • WB

Lab

Western blot - Anti-HLA-DPB1 antibody [EPR11226] (AB157210)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-HLA-DPB1 antibody [EPR11226] (ab157210) at 1/20000 dilution

Lane 1:

Human fetal thymus lysate at 20 µg

Lane 2:

Human tonsil lysate at 20 µg

Lane 3:

Human fetal spleen lysate at 20 µg

Secondary

All lanes:

Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Western blot - Anti-HLA-DPB1 antibody [EPR11226] (AB157210)
  • WB

Unknown

Western blot - Anti-HLA-DPB1 antibody [EPR11226] (AB157210)

All lanes:

Western blot - Anti-HLA-DPB1 antibody [EPR11226] (ab157210) at 1/10000 dilution

All lanes:

Immunoprecipitation pellet from Human fetal thymus lysate at 10 µg

Predicted band size: 29 kDa

false

Western blot - Anti-HLA-DPB1 antibody [EPR11226] (AB157210)
  • WB

Unknown

Western blot - Anti-HLA-DPB1 antibody [EPR11226] (AB157210)

All lanes:

Western blot - Anti-HLA-DPB1 antibody [EPR11226] (ab157210) at 1/10000 dilution

Lane 1:

Human fetal thymus cell lysate at 10 µg

Lane 2:

Human tonsil cell lysate at 10 µg

Predicted band size: 29 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11226

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2500", "IHCP-species-notes": "<p><strong>For unpurified, use 1/100 - 1/250.</strong></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-HLA-DPB1 antibody [EPR11226] (ab157210) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of HLA-DPB1?
Anti-HLA-DPB1 [EPR11226] (ab157210) specifically detects a band for HLA-DPB1 (UniProt: P04440) at a molecular weight of 29kDa.

Trusted by the scientific community
Anti-HLA-DPB1 [EPR11226] (ab157210) was first used in a scientific publication in 2013 and has been cited over 20 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR11226] also available for your convenience: ab157210, Alexa Fluor® 647 - ab201347, Alexa Fluor® 488 - ab201527, Carrier free - ab215973, Alexa Fluor® 594 - ab277933, Alexa Fluor® 555 - ab300098

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
See full target information HLA-DPB1
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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Clinical cancer research : an official journal of the American Association for Cancer Research 30:4601-4608 PubMed39167623

2024

Pembrolizumab and Cabozantinib in Recurrent and/or Metastatic Head and Neck Squamous Cell Carcinoma: Long-term Survival Update with a Biomarker Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Nabil F Saba,Ritu Chaudhary,Kedar Kirtane,Angelo Marra,Asari Ekpenyong,Ashley McCook-Veal,Nicole C Schmitt,Jennifer H Gross,Mihir R Patel,Jill Remick,James E Bates,Mark W McDonald,Soumon F Rudra,William A Stokes,Maria Biernacki,Xiaofei Song,Robbert J C Slebos,Yuan Liu,Conor E Steuer,Dong M Shin,Yong Teng,Christine H Chung

Stem cell reviews and reports 20:1954-1970 PubMed39028417

2024

Trophoblast Side-Population Markers are Dysregulated in Preeclampsia and Fetal Growth Restriction.

Applications

Unspecified application

Species

Unspecified reactive species

Georgia P Wong,Sunhild Hartmann,David G Simmons,Sarah Ellis,Olivia Nonn,Ping Cannon,Tuong-Vi Nguyen,Anna Nguyen,Lucy A Bartho,Stephen Tong,Natalie J Hannan,Tu'uhevaha J Kaitu'u-Lino

Nature communications 14:7479 PubMed37980418

2023

Comprehensive analysis reveals potential therapeutic targets and an integrated risk stratification model for solitary fibrous tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Renjing Zhang,Yang Yang,Chunfang Hu,Mayan Huang,Wenjian Cen,Dongyi Ling,Yakang Long,Xin-Hua Yang,Boheng Xu,Junling Peng,Sujie Wang,Weijie Zhu,Mingbiao Wei,Jiaojiao Yang,Yuxia Xu,Xu Zhang,Jiangjun Ma,Fang Wang,Hongtu Zhang,Peiqing Ma,Xiaojun Zhu,Guohui Song,Li-Yue Sun,De-Shen Wang,Feng-Hua Wang,Yu-Hong Li,Sandro Santagata,Qin Li,Yan-Fen Feng,Ziming Du

Immunity 56:1681-1698.e13 PubMed37301199

2023

HLA-II immunopeptidome profiling and deep learning reveal features of antigenicity to inform antigen discovery.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Stražar,Jihye Park,Jennifer G Abelin,Hannah B Taylor,Thomas K Pedersen,Damian R Plichta,Eric M Brown,Basak Eraslan,Yuan-Mao Hung,Kayla Ortiz,Karl R Clauser,Steven A Carr,Ramnik J Xavier,Daniel B Graham

Cancer research communications 3:896-907 PubMed37377902

2023

EGFR Inhibition by Cetuximab Modulates Hypoxia and IFN Response Genes in Head and Neck Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ritu Chaudhary,Robbert J C Slebos,Leenil C Noel,Feifei Song,Maria I Poole,Dirk S Hoening,Juan C Hernandez-Prera,Jose R Conejo-Garcia,Jose A Guevara-Patino,Xuefeng Wang,Mengyu Xie,Aik Choon Tan,Christine H Chung

Molecular & cellular proteomics : MCP 22:100563 PubMed37142057

2023

Sensitive, High-Throughput HLA-I and HLA-II Immunopeptidomics Using Parallel Accumulation-Serial Fragmentation Mass Spectrometry.

Applications

Unspecified application

Species

Unspecified reactive species

Kshiti Meera Phulphagar,Claudia Ctortecka,Alvaro Sebastian Vaca Jacome,Susan Klaeger,Eva K Verzani,Gabrielle M Hernandez,Namrata D Udeshi,Karl R Clauser,Jennifer G Abelin,Steven A Carr

Nature communications 14:1851 PubMed37012232

2023

Workflow enabling deepscale immunopeptidome, proteome, ubiquitylome, phosphoproteome, and acetylome analyses of sample-limited tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer G Abelin,Erik J Bergstrom,Keith D Rivera,Hannah B Taylor,Susan Klaeger,Charles Xu,Eva K Verzani,C Jackson White,Hilina B Woldemichael,Maya Virshup,Meagan E Olive,Myranda Maynard,Stephanie A Vartany,Joseph D Allen,Kshiti Phulphagar,M Harry Kane,Suzanna Rachimi,D R Mani,Michael A Gillette,Shankha Satpathy,Karl R Clauser,Namrata D Udeshi,Steven A Carr

iScience 25:105358 PubMed36339256

2022

Cellular plasticity and immune microenvironment of malignant pleural effusion are associated with EGFR-TKI resistance in non-small-cell lung carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hyoung-Oh Jeong,Hayoon Lee,Hyemin Kim,Jinho Jang,Seunghoon Kim,Taejoo Hwang,David Whee-Young Choi,Hong Sook Kim,Naeun Lee,Yoo Mi Lee,Sehhoon Park,Hyun Ae Jung,Jong-Mu Sun,Jin Seok Ahn,Myung-Ju Ahn,Keunchil Park,Semin Lee,Se-Hoon Lee

Nature communications 13:5606 PubMed36153324

2022

Interferon gamma mediates the reduction of adipose tissue regulatory T cells in human obesity.

Applications

Unspecified application

Species

Unspecified reactive species

David Bradley,Alan J Smith,Alecia Blaszczak,Dharti Shantaram,Stephen M Bergin,Anahita Jalilvand,Valerie Wright,Kathleen L Wyne,Revati S Dewal,Lisa A Baer,Katherine R Wright,Kristin I Stanford,Bradley Needleman,Stacy Brethauer,Sabrena Noria,David Renton,Joshua J Joseph,Amy Lovett-Racke,Joey Liu,Willa A Hsueh

Blood advances 7:963-970 PubMed35973200

2022

Pembrolizumab for the treatment of disease relapse after allogeneic hematopoietic stem cell transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

James Godfrey,Hongtao Liu,Jovian Yu,Michael Tallarico,Emily Curran,Andrew Artz,Peter A Riedell,Wendy Stock,Theodore Karrison,Carrie Fitzpatrick,Girish Venkataraman,Alan Cooper,Sonali M Smith,Michael R Bishop,Justin Kline
View all publications
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Product promise

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