Anti-HLA-DPB1 antibody [EPR23947-1] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR23947-1] - BSA and Azide free (AB278084)

This data was developed using ab259802, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling HLA-DPB1 with ab259802 at 1/2000 (0.286 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Positive staining on human tonsil. The section was incubated with ab259802 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR23947-1] - BSA and Azide free (AB278084)

This data was developed using ab259802, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling HLA-DPB1 with ab259802 at 1/2000 (0.286 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Positive staining on Kupffer cells in human liver. The section was incubated with ab259802 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR23947-1] - BSA and Azide free (AB278084)

This data was developed using ab259802, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human epityphlon tissue labeling HLA-DPB1 with ab259802 at 1/2000 (0.286 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Positive staining on human epityphlon. The section was incubated with ab259802 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• WB

Lab

Western blot - Anti-HLA-DPB1 antibody [EPR23947-1] - BSA and Azide free (AB278084)

This data was developed using ab259802, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : HeLa.

The expression profile/molecular weight observed is consistent with what has been described in the literature. Band detected around 54 kDa could be the dimeric form (PMID : 20959457).

This blot was developed using a higher sensitivity ECL substrate.

Exposure times : Lane 1 : 81 seconds.

Lane 2-4 : 3 minutes.

All lanes:

Western blot - Anti-HLA-DPB1 antibody [EPR23947-1] (<a href='/en-us/products/primary-antibodies/hla-dpb1-antibody-epr23947-1-ab259802'>ab259802</a>) at 1/1000 dilution

Lane 1:

Daudi (Human Burkitt's lymphoma lymphoblast), whole cell lysate at 20 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate at 20 µg

Lane 3:

Human tonsil tissue lysate at 20 µg

Lane 4:

Human spleen tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

• WB

Lab

Western blot - Anti-HLA-DPB1 antibody [EPR23947-1] - BSA and Azide free (AB278084)

This data was developed using ab259802, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-HLA-DPB1 antibody [EPR23947-1] (<a href='/en-us/products/primary-antibodies/hla-dpb1-antibody-epr23947-1-ab259802'>ab259802</a>) at 1/1000 dilution

Lane 1:

His-tagged human HLA class II histocompatibility antigen, DRB1 beta chain recombinant protein, 35 ng

Lane 2:

HEK-293T transfected with HLA-DPB1 expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 29 kDa

false