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AB227676

Anti-HLA-DPB1 antibody [SP229]

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(1 Publication)

Rabbit Recombinant Monoclonal HLA-DPB1 antibody. Suitable for IHC-P, Flow Cyt (Intra), WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

HLA-DP1B, HLA-DPB1, MHC class II antigen DPB1

6 Images
Flow Cytometry (Intracellular) - Anti-HLA-DPB1 antibody [SP229] (AB227676)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-HLA-DPB1 antibody [SP229] (AB227676)

Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cell line labeling HLA-DPB1 with ab227676 at 1/400 dilution (green) compared with a negative control of rabbit IgG (blue).

Immunocytochemistry/ Immunofluorescence - Anti-HLA-DPB1 antibody [SP229] (AB227676)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HLA-DPB1 antibody [SP229] (AB227676)

Immunocytochemistry/ Immunofluorescence analysis of Ramos (human Burkitt's lymphoma B lymphocyte) cells labeling HLA-DPB1 with purified ab227676 at 1/10 (10 μg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Flow Cytometry (Intracellular) - Anti-HLA-DPB1 antibody [SP229] (AB227676)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-HLA-DPB1 antibody [SP229] (AB227676)

Flow Cytometry analysis of Ramos (Human Burkitt's lymphoma B lymphocyte) cells labeling HLA-DPB1 with purified ab227676 at 1/20 dilution (5.5 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [SP229] (AB227676)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [SP229] (AB227676)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling HLA-DPB1 with ab227676 at 1/100 dilution (1.10 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [SP229] (AB227676)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [SP229] (AB227676)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for HLA-DPB1 using ab227676 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-HLA-DPB1 antibody [SP229] (AB227676)
  • WB

Unknown

Western blot - Anti-HLA-DPB1 antibody [SP229] (AB227676)

Blocking/diluting Buffer and concentration : 5% NFDM /TBST

All lanes:

Western blot - Anti-HLA-DPB1 antibody [SP229] (ab227676) at 0.11 µg/mL

Lane 1:

Ramos (Human Burkitt's lymphoma B lymphocyte) at 20 µg

Lane 2:

Raji (Human Burkitt's lymphoma B lymphocyte) at 20 µg

Lane 3:

HeLa (Human cervix adenocarcinoma epithelial cell) at 20 µg

Lane 4:

Human spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 0.05 µg/mL

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

  • Carrier free

    Anti-HLA-DPB1 antibody [SP229] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP229

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Primary antibody incubation for 10 minutes at room temperature.</p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20 - 1/400", "FlowCytIntra-species-notes": "<p>Primary antibody incubation for 10 minutes at 4°C.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.11 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/10", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by Protein A/G.
Storage buffer
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
See full target information HLA-DPB1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The European respiratory journal 63: PubMed38212075

2024

Single-cell transcriptomic analysis of human pleura reveals stromal heterogeneity and informs models of mesothelioma.

Applications

Unspecified application

Species

Unspecified reactive species

Joanna Obacz,Jose Antonio Valer,Reshma Nibhani,Taylor S Adams,Jonas C Schupp,Niki Veale,Amanah Lewis-Wade,Jasper Flint,John Hogan,Giuseppe Aresu,Aman S Coonar,Adam Peryt,Giulia Biffi,Naftali Kaminski,Hayley Francies,Doris M Rassl,Mathew J Garnett,Robert C Rintoul,Stefan J Marciniak
View all publications
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Product promise

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