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AB128959

Anti-HLA-DQA1 antibody [EPR7300]

5

(1 Review)

|

(7 Publications)

Rabbit Recombinant Monoclonal DQA1 antibody. Suitable for IP, IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 7 publications.

View Alternative Names

DC-1 alpha chain, DC-alpha, HLA-DCA, MHC class II DQA1, HLA-DQA1

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DQA1 antibody [EPR7300] (AB128959)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DQA1 antibody [EPR7300] (AB128959)

Immunohistochemical analysis of Paraffin-embedded sections human tonsil tissue labelling HLA-DQA1 with ab128959 at 1/200 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on human tonsil tissue is observed. Counter stained with Haematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). The section was incubated with ab128959 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DQA1 antibody [EPR7300] (AB128959)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DQA1 antibody [EPR7300] (AB128959)

Immunohistochemical analysis of Paraffin-embedded sections human liver tissue labelling HLA-DQA1 with ab128959 at 1/200 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on human liver tissue is observed. Counter stained with Haematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). The section was incubated with ab128959 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Immunoprecipitation - Anti-HLA-DQA1 antibody [EPR7300] (AB128959)
  • IP

Lab

Immunoprecipitation - Anti-HLA-DQA1 antibody [EPR7300] (AB128959)

Immunoprecipitation of HLA-DQA1 from 2Human spleen lysate using ab128959 at 1/20 dilution. Lane 1 : Human spleen lysate 10 μg Lane 2 : Human spleen lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab128959 in Human spleen lysate ab131366 was used as secondary antibody at 1/1000 dilution. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Observed MW at 33-35kDa.

All lanes:

Immunoprecipitation - Anti-HLA-DQA1 antibody [EPR7300] (ab128959) at 1/20 dilution

Lane 1:

Human spleen lysate at 10 µg

Lane 2:

Human spleen lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 28 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DQA1 antibody [EPR7300] (AB128959)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DQA1 antibody [EPR7300] (AB128959)

Immunohistochemical analysis of Paraffin-embedded sections rat spleen tissue labelling HLA-DQA1 with ab128959 at 1/200 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on rat spleen tissue is observed. Counter stained with Haematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). The section was incubated with ab128959 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DQA1 antibody [EPR7300] (AB128959)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DQA1 antibody [EPR7300] (AB128959)

Immunohistochemical analysis of Paraffin-embedded sections mouse spleen tissue labelling HLA-DQA1 with ab128959 at 1/200 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on mouse spleen tissue is observed. Counter stained with Haematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). The section was incubated with ab128959 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Western blot - Anti-HLA-DQA1 antibody [EPR7300] (AB128959)
  • WB

Lab

Western blot - Anti-HLA-DQA1 antibody [EPR7300] (AB128959)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-HLA-DQA1 antibody [EPR7300] (ab128959) at 1/1000 dilution

Lane 1:

Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Lane 2:

Mouse spleen lysate at 20 µg

Lane 3:

Rat spleen lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 28 kDa

false

  • Carrier free

    Anti-HLA-DQA1 antibody [EPR7300] - BSA and Azide free

  • 660 APC

    APC Anti-HLA-DQA1 antibody [EPR7300]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-HLA-DQA1 antibody [EPR7300]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-HLA-DQA1 antibody [EPR7300]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-HLA-DQA1 antibody [EPR7300]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-HLA-DQA1 antibody [EPR7300]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-HLA-DQA1 antibody [EPR7300]

  • 578 PE

    PE Anti-HLA-DQA1 antibody [EPR7300]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7300

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>Signals detected in mouse and rat samples are orthologs of HLA.</p>

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Complementary Products

Primary Antibodies

AB170867

Anti-MHC Class II beta antibody [EPR11227]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC Class II beta antibody [EPR11227] (AB170867)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
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Primary Antibodies

AB92511

Anti-HLA-DR antibody [EPR3692]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] (AB92511)

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Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p>Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p>Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p>Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
See full target information HLA-DQA1
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 29:145 PubMed37884883

2023

Leveraging a disulfidptosis-related signature to predict the prognosis and immunotherapy effectiveness of cutaneous melanoma based on machine learning.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhao,Yanjun Wei,Lingjia Fan,Yuanliu Nie,Jianan Li,Renya Zeng,Jixian Li,Xiang Zhan,Lingli Lei,Zhichao Kang,Jiaxin Li,Wentao Zhang,Zhe Yang

iScience 25:105358 PubMed36339256

2022

Cellular plasticity and immune microenvironment of malignant pleural effusion are associated with EGFR-TKI resistance in non-small-cell lung carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hyoung-Oh Jeong,Hayoon Lee,Hyemin Kim,Jinho Jang,Seunghoon Kim,Taejoo Hwang,David Whee-Young Choi,Hong Sook Kim,Naeun Lee,Yoo Mi Lee,Sehhoon Park,Hyun Ae Jung,Jong-Mu Sun,Jin Seok Ahn,Myung-Ju Ahn,Keunchil Park,Semin Lee,Se-Hoon Lee

Frontiers in oncology 12:845765 PubMed35936748

2022

Differential Expression and Prognostic Correlation of Immune Related Factors Between Right and Left Side Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Hu,Jie Ding,Chengjiang Wu,Hong Gao,Meiling Ge,Qixiang Shao,Yanhong Liu,Qing Ye

Nature communications 13:896 PubMed35173148

2022

Proteomic analysis of archival breast cancer clinical specimens identifies biological subtypes with distinct survival outcomes.

Applications

Unspecified application

Species

Unspecified reactive species

Karama Asleh,Gian Luca Negri,Sandra E Spencer Miko,Shane Colborne,Christopher S Hughes,Xiu Q Wang,Dongxia Gao,C Blake Gilks,Stephen K L Chia,Torsten O Nielsen,Gregg B Morin

Experimental and therapeutic medicine 20:107 PubMed32989386

2020

Evaluation of immune-biomarker expression in high-grade soft-tissue sarcoma: HLA-DQA1 expression as a prognostic marker.

Applications

Unspecified application

Species

Unspecified reactive species

Jung Yun Bae,Kyung Un Choi,Ahrong Kim,So Jung Lee,Kyungbin Kim,Jee Yeon Kim,In Sook Lee,So Hak Chung,Jeung Il Kim

Human immunology 79:558-563 PubMed29656111

2018

Circulating histocompatibility antigen (HLA) gene products may help differentiate benign from malignant indeterminate pulmonary lesions.

Applications

WB

Species

Unspecified reactive species

Smriti Kanangat,Christopher W Seder,Melissa R Pergande,Gabriela C Lobato,Cristina L Fhied,Maryam F Raouf,Michael J Liptay,Jeffrey A Borgia

The Journal of biological chemistry 290:15260-78 PubMed25911099

2015

Role of a Novel Human Leukocyte Antigen-DQA1*01:02;DRB1*15:01 Mixed Isotype Heterodimer in the Pathogenesis of "Humanized" Multiple Sclerosis-like Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Nathali Kaushansky,Miriam Eisenstein,Sigalit Boura-Halfon,Bjarke Endel Hansen,Claus Henrik Nielsen,Ron Milo,Gabriel Zeilig,Hans Lassmann,Daniel M Altmann,Avraham Ben-Nun
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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