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Rabbit Recombinant Monoclonal HLA-DRA antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), mIHC and reacts with Transfected cell line - Human, Human samples. Cited in 1 publication.


Images

Immunocytochemistry/ Immunofluorescence - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (AB209968), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (AB209968), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (AB209968), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (AB209968), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (AB209968), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFFlow Cyt (Intra)mIHC
Human
Not recommended
Tested
Tested
Tested
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Primates
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell line - Human
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species

Primates, Human, Transfected cell line - Human, Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Transfected cell line - Human, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Primates

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Primates, Transfected cell line - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Primates, Transfected cell line - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Primates, Transfected cell line - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Primates, Transfected cell line - Human, Mouse

Dilution info

-

Notes

-

Associated Products

Select an associated product type

4 products for Alternative Version

Target data

Function

An alpha chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the beta chain HLA-DRB, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell receptor (TCR) on HLA-DR-restricted CD4-positive T cells. This guides antigen-specific T-helper effector functions, both antibody-mediated immune response and macrophage activation, to ultimately eliminate the infectious agents and transformed cells (PubMed:29884618, PubMed:17334368, PubMed:8145819, PubMed:15322540, PubMed:22327072, PubMed:27591323, PubMed:31495665, PubMed:15265931, PubMed:9075930, PubMed:24190431). Typically presents extracellular peptide antigens of 10 to 30 amino acids that arise from proteolysis of endocytosed antigens in lysosomes (PubMed:8145819). In the tumor microenvironment, presents antigenic peptides that are primarily generated in tumor-resident APCs likely via phagocytosis of apoptotic tumor cells or macropinocytosis of secreted tumor proteins (PubMed:31495665). Presents peptides derived from intracellular proteins that are trapped in autolysosomes after macroautophagy, a mechanism especially relevant for T cell selection in the thymus and central immune tolerance (PubMed:17182262, PubMed:23783831). The selection of the immunodominant epitopes follows two processing modes: 'bind first, cut/trim later' for pathogen-derived antigenic peptides and 'cut first, bind later' for autoantigens/self-peptides (PubMed:25413013). The anchor residue at position 1 of the peptide N-terminus, usually a large hydrophobic residue, is essential for high affinity interaction with MHCII molecules (PubMed:8145819).

Alternative names

MHC class II antigen DRA, HLA-DRA, HLA-DRA1

Recommended products

Rabbit Recombinant Monoclonal HLA-DRA antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), mIHC and reacts with Transfected cell line - Human, Human samples. Cited in 1 publication.

Alternative names

MHC class II antigen DRA, HLA-DRA, HLA-DRA1

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR3692

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab209968 is the carrier-free version of Anti-HLA-DR antibody [EPR3692] ab92511.

Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

15 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (ab209968), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (ab209968)

    Anti-HLA-DR antibody [EPR3692] ab92511 staining HLA-DR in HuT-78 (human Sezary syndrome) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a dilution of 1/1000. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 were used as counterstains for primary antibody Anti-HLA-DR antibody [EPR3692] ab92511 and secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 respectively and DAPI was used as a nuclear counterstain.

    Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120)
    Negative control 2: Mouse primary antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) and anti-rabbit secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HLA-DR antibody [EPR3692] ab92511).

  • Flow Cytometry (Intracellular) - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (ab209968), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (ab209968)

    Overlay histogram showing Raji cells stained with Anti-HLA-DR antibody [EPR3692] ab92511 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-HLA-DR antibody [EPR3692] ab92511, 1/50) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HLA-DR antibody [EPR3692] ab92511).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (ab209968), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (ab209968)

    Anti-HLA-DR antibody [EPR3692] ab92511 staining HLA-DR in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/700. A goat anti-rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HLA-DR antibody [EPR3692] ab92511).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (ab209968), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (ab209968)

    Anti-HLA-DR antibody [EPR3692] ab92511 showing positive staining in Normal liver vessels tissue.


    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HLA-DR antibody [EPR3692] ab92511).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (ab209968), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (ab209968)

    Anti-HLA-DR antibody [EPR3692] ab92511 showing positive staining in Normal skin vessels tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HLA-DR antibody [EPR3692] ab92511).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (ab209968), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (ab209968)

    Anti-HLA-DR antibody [EPR3692] ab92511 showing positive staining in Endometrial carcinoma vessels tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HLA-DR antibody [EPR3692] ab92511).

  • OI-RD Scanning - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (ab209968), expandable thumbnail

    OI-RD Scanning - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (ab209968)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (ab209968), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (ab209968)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labelling HLA-DR with Anti-HLA-DR antibody [EPR3692] ab92511 at 1/10000 dilution (0.07 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) secondary antibody. Positive staining on human tonsil tissue. The section was incubated with Anti-HLA-DR antibody [EPR3692] ab92511 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control.

    Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 30 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HLA-DR antibody [EPR3692] ab92511).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (ab209968), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (ab209968)

    Immunohistochemical analysis of paraffin-embedded human skin tissue labelling HLA-DR with Anti-HLA-DR antibody [EPR3692] ab92511 at 1/10000 dilution (0.07 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) secondary antibody. Positive staining on human skin tissue. The section was incubated with Anti-HLA-DR antibody [EPR3692] ab92511 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control.

    Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 30 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HLA-DR antibody [EPR3692] ab92511).

  • Western blot - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (ab209968), expandable thumbnail

    Western blot - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (ab209968)

    Blocking buffer and concentration : 5% NFDM/TBST
    Diluting buffer and concentration : 5% NFDM/TBST
    This antibody does not cross-react with human HLA-DOA.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HLA-DR antibody [EPR3692] ab92511).

    Lanes 1 - 2: Western blot - Anti-HLA-DR antibody [EPR3692] - Low endotoxin, Azide free (Anti-HLA-DR antibody [EPR3692] - Low endotoxin, Azide free ab215985) at 1/1000 dilution

    Lanes 1 - 2: Western blot - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (ab209968) at 1/1000 dilution

    Lanes 1 - 2: Western blot - Anti-HLA-DR antibody [EPR3692] (Anti-HLA-DR antibody [EPR3692] ab92511) at 1/1000 dilution

    Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with a human HLA-DR expression vector containing a his-tag, whole cell lysate at 20 µg

    Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with a human HLA-DOA expression vector containing a his-tag, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 29 kDa

    Observed band size: 37 kDa

    Exposure time: 3min

    Blocking buffer and concentration : 5% NFDM/TBST
    Diluting buffer and concentration : 5% NFDM/TBST
    This antibody does not cross-react with human HLA-DOA.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (ab209968), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (ab209968)

    Anti-HLA-DR antibody [EPR3692] ab92511, at a 1/100 dilution, staining HLA-DRA in paraffin embedded Human tonsil (A) and Human kidney (B) tissue by Immunohistochemistry. Detection: by DAB staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HLA-DR antibody [EPR3692] ab92511).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (ab209968), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (ab209968)

    Anti-HLA-DR antibody [EPR3692] ab92511 showing positive staining in Endometrial carcinoma vessels tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HLA-DR antibody [EPR3692] ab92511).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (ab209968), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (ab209968)

    Anti-HLA-DR antibody [EPR3692] ab92511 showing positive staining in Normal skin vessels tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HLA-DR antibody [EPR3692] ab92511).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (ab209968), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (ab209968)

    Anti-HLA-DR antibody [EPR3692] ab92511 showing positive staining in Normal liver vessels tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HLA-DR antibody [EPR3692] ab92511).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (ab209968), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [EPR3692] - BSA and Azide free (ab209968)

    Anti-HLA-DR antibody [EPR3692] ab92511 staining HLA-DR in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/700. A goat anti-rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HLA-DR antibody [EPR3692] ab92511).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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