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Rabbit Recombinant Monoclonal HLA-DRA antibody. Carrier free. Suitable for IHC-P, Flow Cyt, Protein Array, ICC/IF and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Cell preparation containing HLA-DRA protein.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-HLA-DR antibody [HLA-Pan/2967R] - BSA and Azide free (AB259258), expandable thumbnail
  • Flow Cytometry - Anti-HLA-DR antibody [HLA-Pan/2967R] - BSA and Azide free (AB259258), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [HLA-Pan/2967R] - BSA and Azide free (AB259258), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-HLA-DR antibody [HLA-Pan/2967R] - BSA and Azide free (AB259258), expandable thumbnail
  • Protein Array - Anti-HLA-DR antibody [HLA-Pan/2967R] - BSA and Azide free (AB259258), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Cell preparation containing HLA-DRA protein. The exact immunogen used to generate this antibody is proprietary information. Database link P01903

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow CytProtein ArrayICC/IF
Human
Tested
Tested
Expected
Tested
Recombinant full length protein - Human
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1-2 µg/mL
Notes

30 minutes at RT

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1-2 µg for 106 Cells
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1-2 µg/mL
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

An alpha chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the beta chain HLA-DRB, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell receptor (TCR) on HLA-DR-restricted CD4-positive T cells. This guides antigen-specific T-helper effector functions, both antibody-mediated immune response and macrophage activation, to ultimately eliminate the infectious agents and transformed cells (PubMed:15265931, PubMed:15322540, PubMed:17334368, PubMed:22327072, PubMed:24190431, PubMed:27591323, PubMed:29884618, PubMed:31495665, PubMed:8145819, PubMed:9075930). Typically presents extracellular peptide antigens of 10 to 30 amino acids that arise from proteolysis of endocytosed antigens in lysosomes (PubMed:8145819). In the tumor microenvironment, presents antigenic peptides that are primarily generated in tumor-resident APCs likely via phagocytosis of apoptotic tumor cells or macropinocytosis of secreted tumor proteins (PubMed:31495665). Presents peptides derived from intracellular proteins that are trapped in autolysosomes after macroautophagy, a mechanism especially relevant for T cell selection in the thymus and central immune tolerance (PubMed:17182262, PubMed:23783831). The selection of the immunodominant epitopes follows two processing modes: 'bind first, cut/trim later' for pathogen-derived antigenic peptides and 'cut first, bind later' for autoantigens/self-peptides (PubMed:25413013). The anchor residue at position 1 of the peptide N-terminus, usually a large hydrophobic residue, is essential for high affinity interaction with MHCII molecules (PubMed:8145819).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal HLA-DRA antibody. Carrier free. Suitable for IHC-P, Flow Cyt, Protein Array, ICC/IF and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Cell preparation containing HLA-DRA protein.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Cell preparation containing HLA-DRA protein. The exact immunogen used to generate this antibody is proprietary information. Database link P01903
Carrier free
Yes
Clone number
HLA-Pan/2967R
Purification technique
Affinity purification Protein A/G
Concentration
Loading...
Purification notes

Purified from Bioreactor Concentrate.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

ab259258 is the carrier-free version of Anti-HLA-DR antibody [HLA-Pan/2967R] ab257320.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-HLA-DR antibody [HLA-Pan/2967R] - BSA and Azide free (ab259258), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-HLA-DR antibody [HLA-Pan/2967R] - BSA and Azide free (ab259258)

    Immunocytochemistry/ Immunofluorescent analysis of Ramos cells staining HLA-DR with Anti-HLA-DR antibody [HLA-Pan/2967R] ab257320 at 2μg/ml, followed by a goat anti-rabbit IgG-CF488 secondary (green). Nuceli stained with reddot (red).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-HLA-DR antibody [HLA-Pan/2967R] ab257320).

  • Flow Cytometry - Anti-HLA-DR antibody [HLA-Pan/2967R] - BSA and Azide free (ab259258), expandable thumbnail

    Flow Cytometry - Anti-HLA-DR antibody [HLA-Pan/2967R] - BSA and Azide free (ab259258)

    Flow Cytometric analysis of Raji cells staining HLA-DR with Anti-HLA-DR antibody [HLA-Pan/2967R] ab257320, followed by a goat anti-rabbit IgG-CF488 secondary antiobdy (blue). Isotype control (red).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-HLA-DR antibody [HLA-Pan/2967R] ab257320).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [HLA-Pan/2967R] - BSA and Azide free (ab259258), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [HLA-Pan/2967R] - BSA and Azide free (ab259258)

    Immunohistochemical analysis of formalin fixed, paraffin-embedded human tonsil staining HLA-DR with Anti-HLA-DR antibody [HLA-Pan/2967R] ab257320 at 2μg/ml for 30 minutes at room temperature.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-HLA-DR antibody [HLA-Pan/2967R] ab257320).

  • Immunocytochemistry/ Immunofluorescence - Anti-HLA-DR antibody [HLA-Pan/2967R] - BSA and Azide free (ab259258), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-HLA-DR antibody [HLA-Pan/2967R] - BSA and Azide free (ab259258)

    Immunocytochemistry/ Immunofluorescent analysis of Ramos cells staining HLA-DR with Anti-HLA-DR antibody [HLA-Pan/2967R] ab257320 at 2μg/ml, followed by a goat anti-rabbit IgG-CF488 secondary (blue) and an isotype control (red).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-HLA-DR antibody [HLA-Pan/2967R] ab257320).

  • Protein Array - Anti-HLA-DR antibody [HLA-Pan/2967R] - BSA and Azide free (ab259258), expandable thumbnail

    Protein Array - Anti-HLA-DR antibody [HLA-Pan/2967R] - BSA and Azide free (ab259258)

    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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