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AB259258

Anti-HLA-DR antibody [HLA-Pan/2967R] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal HLA-DRA antibody. Carrier free. Suitable for IHC-P, Flow Cyt, Protein Array, ICC/IF and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing HLA-DRA protein.

View Alternative Names

HLA-DRA1, HLA-DRA, MHC class II antigen DRA

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [HLA-Pan/2967R] - BSA and Azide free (AB259258)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [HLA-Pan/2967R] - BSA and Azide free (AB259258)

Immunohistochemical analysis of formalin fixed, paraffin-embedded human tonsil staining HLA-DR with ab257320 at 2μg/ml for 30 minutes at room temperature.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab257320).

Flow Cytometry - Anti-HLA-DR antibody [HLA-Pan/2967R] - BSA and Azide free (AB259258)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-HLA-DR antibody [HLA-Pan/2967R] - BSA and Azide free (AB259258)

Flow Cytometric analysis of Raji cells staining HLA-DR with ab257320, followed by a goat anti-rabbit IgG-CF488 secondary antiobdy (blue). Isotype control (red).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab257320).

Immunocytochemistry/ Immunofluorescence - Anti-HLA-DR antibody [HLA-Pan/2967R] - BSA and Azide free (AB259258)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HLA-DR antibody [HLA-Pan/2967R] - BSA and Azide free (AB259258)

Immunocytochemistry/ Immunofluorescent analysis of Ramos cells staining HLA-DR with ab257320 at 2μg/ml, followed by a goat anti-rabbit IgG-CF488 secondary (blue) and an isotype control (red).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab257320).

Immunocytochemistry/ Immunofluorescence - Anti-HLA-DR antibody [HLA-Pan/2967R] - BSA and Azide free (AB259258)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HLA-DR antibody [HLA-Pan/2967R] - BSA and Azide free (AB259258)

Immunocytochemistry/ Immunofluorescent analysis of Ramos cells staining HLA-DR with ab257320 at 2μg/ml, followed by a goat anti-rabbit IgG-CF488 secondary (green). Nuceli stained with reddot (red).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab257320).

Protein Array - Anti-HLA-DR antibody [HLA-Pan/2967R] - BSA and Azide free (AB259258)
  • Protein Array

Supplier Data

Protein Array - Anti-HLA-DR antibody [HLA-Pan/2967R] - BSA and Azide free (AB259258)

Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HLA-Pan/2967R

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt, Protein Array, ICC/IF, IHC-P

applications

Immunogen

Cell preparation containing HLA-DRA protein. The exact immunogen used to generate this antibody is proprietary information.

P01903

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>30 minutes at RT</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p></p>", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Recombinant full length protein - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

Product details

ab259258 is the carrier-free version of ab257320.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from Bioreactor Concentrate.
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

An alpha chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the beta chain HLA-DRB, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell receptor (TCR) on HLA-DR-restricted CD4-positive T cells. This guides antigen-specific T-helper effector functions, both antibody-mediated immune response and macrophage activation, to ultimately eliminate the infectious agents and transformed cells (PubMed : 15265931, PubMed : 15322540, PubMed : 17334368, PubMed : 22327072, PubMed : 24190431, PubMed : 27591323, PubMed : 29884618, PubMed : 31495665, PubMed : 8145819, PubMed : 9075930). Typically presents extracellular peptide antigens of 10 to 30 amino acids that arise from proteolysis of endocytosed antigens in lysosomes (PubMed : 8145819). In the tumor microenvironment, presents antigenic peptides that are primarily generated in tumor-resident APCs likely via phagocytosis of apoptotic tumor cells or macropinocytosis of secreted tumor proteins (PubMed : 31495665). Presents peptides derived from intracellular proteins that are trapped in autolysosomes after macroautophagy, a mechanism especially relevant for T cell selection in the thymus and central immune tolerance (PubMed : 17182262, PubMed : 23783831). The selection of the immunodominant epitopes follows two processing modes : 'bind first, cut/trim later' for pathogen-derived antigenic peptides and 'cut first, bind later' for autoantigens/self-peptides (PubMed : 25413013). The anchor residue at position 1 of the peptide N-terminus, usually a large hydrophobic residue, is essential for high affinity interaction with MHCII molecules (PubMed : 8145819).
See full target information HLA-DRA

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer immunology, immunotherapy : CII 73:78 PubMed38554152

2024

Lipid droplets-related Perilipin-3: potential immune checkpoint and oncogene in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yijia He,Lingyun Liu,Yuexin Dong,Xiaoxin Zhang,Yuxian Song,Yue Jing,Yanhong Ni,Yi Wang,Zhiyong Wang,Liang Ding
View all publications

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