Rabbit Recombinant Monoclonal HLA-DRA antibody. Carrier free. Suitable for IHC-P, Flow Cyt, Protein Array, ICC/IF and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Cell preparation containing HLA-DRA protein.
pH: 7.2 - 7.4
Constituents: PBS
IHC-P | Flow Cyt | Protein Array | ICC/IF | |
---|---|---|---|---|
Human | Tested | Tested | Expected | Tested |
Recombinant full length protein - Human | Not recommended | Not recommended | Tested | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes 30 minutes at RT Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1-2 µg for 106 Cells | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
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An alpha chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the beta chain HLA-DRB, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell receptor (TCR) on HLA-DR-restricted CD4-positive T cells. This guides antigen-specific T-helper effector functions, both antibody-mediated immune response and macrophage activation, to ultimately eliminate the infectious agents and transformed cells (PubMed:15265931, PubMed:15322540, PubMed:17334368, PubMed:22327072, PubMed:24190431, PubMed:27591323, PubMed:29884618, PubMed:31495665, PubMed:8145819, PubMed:9075930). Typically presents extracellular peptide antigens of 10 to 30 amino acids that arise from proteolysis of endocytosed antigens in lysosomes (PubMed:8145819). In the tumor microenvironment, presents antigenic peptides that are primarily generated in tumor-resident APCs likely via phagocytosis of apoptotic tumor cells or macropinocytosis of secreted tumor proteins (PubMed:31495665). Presents peptides derived from intracellular proteins that are trapped in autolysosomes after macroautophagy, a mechanism especially relevant for T cell selection in the thymus and central immune tolerance (PubMed:17182262, PubMed:23783831). The selection of the immunodominant epitopes follows two processing modes: 'bind first, cut/trim later' for pathogen-derived antigenic peptides and 'cut first, bind later' for autoantigens/self-peptides (PubMed:25413013). The anchor residue at position 1 of the peptide N-terminus, usually a large hydrophobic residue, is essential for high affinity interaction with MHCII molecules (PubMed:8145819).
HLA-DRA1, HLA-DRA, MHC class II antigen DRA
Rabbit Recombinant Monoclonal HLA-DRA antibody. Carrier free. Suitable for IHC-P, Flow Cyt, Protein Array, ICC/IF and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Cell preparation containing HLA-DRA protein.
pH: 7.2 - 7.4
Constituents: PBS
Purified from Bioreactor Concentrate.
ab259258 is the carrier-free version of Anti-HLA-DR antibody [HLA-Pan/2967R] ab257320.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunocytochemistry/ Immunofluorescent analysis of Ramos cells staining HLA-DR with Anti-HLA-DR antibody [HLA-Pan/2967R] ab257320 at 2μg/ml, followed by a goat anti-rabbit IgG-CF488 secondary (green). Nuceli stained with reddot (red).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-HLA-DR antibody [HLA-Pan/2967R] ab257320).
Flow Cytometric analysis of Raji cells staining HLA-DR with Anti-HLA-DR antibody [HLA-Pan/2967R] ab257320, followed by a goat anti-rabbit IgG-CF488 secondary antiobdy (blue). Isotype control (red).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-HLA-DR antibody [HLA-Pan/2967R] ab257320).
Immunohistochemical analysis of formalin fixed, paraffin-embedded human tonsil staining HLA-DR with Anti-HLA-DR antibody [HLA-Pan/2967R] ab257320 at 2μg/ml for 30 minutes at room temperature.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-HLA-DR antibody [HLA-Pan/2967R] ab257320).
Immunocytochemistry/ Immunofluorescent analysis of Ramos cells staining HLA-DR with Anti-HLA-DR antibody [HLA-Pan/2967R] ab257320 at 2μg/ml, followed by a goat anti-rabbit IgG-CF488 secondary (blue) and an isotype control (red).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-HLA-DR antibody [HLA-Pan/2967R] ab257320).
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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