Anti-HLA DR + DP + DQ antibody [CR3/43]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] (AB7856)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using ab7856 at a 1/18000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human tonsil.
The section was incubated with ab7856 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument
Hematoxylin was used as a counter stain.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] (AB7856)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue using ab7856 at a 1/18000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human skeletal muscle.
The section was incubated with ab7856 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument
Hematoxylin was used as a counter stain.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] (AB7856)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue using ab7856 at a 1/18000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human spleen.
The section was incubated with ab7856 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument
Hematoxylin was used as a counter stain.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• Flow Cyt

Lab

Flow Cytometry - Anti-HLA DR + DP + DQ antibody [CR3/43] (AB7856)

Flow cytometric analysis of KARPAS-299 (human T cell lymphoma cell) (Right) / HepG2 (human hepatocellular carcinoma epithelial cell) (Left) cells labelling HLA DR + DP + DQ with ab7856 at 1/1000 dilution (0.1&micor;g)/Red followed by a secondary antibody Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) used at a 1/5000 dilution compared with isotype control Rabbit monoclonal IgG (ab172730) / Black and unlabelled control Cell without incubation with primary antibody and secondary antibody / Blue.

Gated on viable cells.
Negative control : HepG2.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] (AB7856)

Immunohistochemical analysis of paraffin-embedded Human colon tissue using ab7856 at a 1/18000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human colon.
The section was incubated with ab7856 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument
Hematoxylin was used as a counter stain.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• WB

Lab

Western blot - Anti-HLA DR + DP + DQ antibody [CR3/43] (AB7856)

Blocking and dilution buffer and concentration : 5% NFDM/TBST

Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) was used as a loading control at 1/200000 dilution.

Low expression : skeletal muscle.
The identity of MW band at approximately 50 kDa (in lane 2) is unknown.

All lanes:

Western blot - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856) at 1/1000 dilution

Lane 1:

Human tonsil tissue lysate at 20 µg

Lane 2:

Human lung tissue lysate at 20 µg

Lane 3:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 30 kDa

false

Exposure time: 37s

• WB

Lab

Western blot - Anti-HLA DR + DP + DQ antibody [CR3/43] (AB7856)

Blocking and dilution buffer and concentration : 5% NFDM/TBST

Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) was used as a loading control at 1/200000 dilution.

Low expression : HeLa.
Negative control : HepG2.

All lanes:

Western blot - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856) at 1/1000 dilution

Lane 1:

U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

KARPAS-299 (human T cell lymphoma cell) whole cell lysate at 20 µg

Lane 3:

HDLM-2 (human Hodgkin lymphoma cell) whole cell lysate at 20 µg

Lane 4:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 30 kDa

false

Exposure time: 10s