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AB324796

Anti-HLA DR + DP + DQ antibody [CR3/43] - BSA and Azide free

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Mouse Recombinant Monoclonal HLA-DRA antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing HLA-DPB1 protein.

View Alternative Names

HLA-DRA1, HLA-DRA, MHC class II antigen DRA

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] - BSA and Azide free (AB324796)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] - BSA and Azide free (AB324796)

This data was developed using ab7856, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using ab7856 at a 1/18000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human tonsil.
The section was incubated with ab7856 for 30 mins at room temperature followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Hematoxylin was used as a counter stain.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0 Epitope Retrieval Solution 1) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] - BSA and Azide free (AB324796)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] - BSA and Azide free (AB324796)

This data was developed using ab7856, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue using ab7856 at a 1/18000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human skeletal muscle.
The section was incubated with ab7856 for 30 mins at room temperature followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Hematoxylin was used as a counter stain.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0 Epitope Retrieval Solution 1) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] - BSA and Azide free (AB324796)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] - BSA and Azide free (AB324796)

This data was developed using ab7856, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human spleen tissue using ab7856 at a 1/18000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human spleen.
The section was incubated with ab7856 for 30 mins at room temperature followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Hematoxylin was used as a counter stain.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0 Epitope Retrieval Solution 1) for 20 mins.

Flow Cytometry - Anti-HLA DR + DP + DQ antibody [CR3/43] - BSA and Azide free (AB324796)
  • Flow Cyt

Lab

Flow Cytometry - Anti-HLA DR + DP + DQ antibody [CR3/43] - BSA and Azide free (AB324796)

This data was developed using ab7856, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of KARPAS-299 (human T cell lymphoma cell) (Right) / HepG2 (human hepatocellular carcinoma epithelial cell) (Left) cells labelling HLA DR + DP + DQ with ab7856 at 1/1000 dilution (0.1&micor;g)/Red followed by a secondary antibody Goat Anti-Rabbit IgG (Alexa Fluor® 488 ab150081) used at a 1/5000 dilution compared with isotype control Rabbit monoclonal IgG (ab172730) / Black and unlabelled control Cell without incubation with primary antibody and secondary antibody / Blue.

Gated on viable cells.
Negative control : HepG2.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] - BSA and Azide free (AB324796)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] - BSA and Azide free (AB324796)

This data was developed using ab7856, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue using ab7856 at a 1/18000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human colon.
The section was incubated with ab7856 for 30 mins at room temperature followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Hematoxylin was used as a counter stain.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0 Epitope Retrieval Solution 1) for 20 mins.

Western blot - Anti-HLA DR + DP + DQ antibody [CR3/43] - BSA and Azide free (AB324796)
  • WB

Lab

Western blot - Anti-HLA DR + DP + DQ antibody [CR3/43] - BSA and Azide free (AB324796)

This data was developed using ab7856, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) was used as a loading control at 1/200000 dilution.

Low expression : skeletal muscle.
The identity of MW band at approximately 50 kDa (in lane 2) is unknown.

All lanes:

Western blot - Anti-HLA DR + DP + DQ antibody [CR3/43] (<a href='/en-us/products/primary-antibodies/hla-dr-dp-dq-antibody-cr3-43-ab7856'>ab7856</a>) at 1/1000 dilution

Lane 1:

Human tonsil tissue lysate at 20 µg

Lane 2:

Human lung tissue lysate at 20 µg

Lane 3:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 30 kDa

false

Exposure time: 37s

Western blot - Anti-HLA DR + DP + DQ antibody [CR3/43] - BSA and Azide free (AB324796)
  • WB

Lab

Western blot - Anti-HLA DR + DP + DQ antibody [CR3/43] - BSA and Azide free (AB324796)

This data was developed using ab7856, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) was used as a loading control at 1/200000 dilution.

Low expression : HeLa.
Negative control : HepG2.

All lanes:

Western blot - Anti-HLA DR + DP + DQ antibody [CR3/43] (<a href='/en-us/products/primary-antibodies/hla-dr-dp-dq-antibody-cr3-43-ab7856'>ab7856</a>) at 1/1000 dilution

Lane 1:

U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

KARPAS-299 (human T cell lymphoma cell) whole cell lysate at 20 µg

Lane 3:

HDLM-2 (human Hodgkin lymphoma cell) whole cell lysate at 20 µg

Lane 4:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 30 kDa

false

Exposure time: 10s

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CR3/43

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt, IHC-P, WB

applications

Immunogen

Cell preparation containing HLA-DPB1 protein. The exact immunogen used to generate this antibody is proprietary information.

P04440

Specificity

The gene of the human histocompatibility complex class II consists of at least four subregions: HLA DP, DQ, and DR, containing a minimum of one alpha and one beta chain. This antibody reacts with the beta-chain of all products of the gene subregions DP, DQ, and DR.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

ab324796 is the carrier-free version of ab7856.

Product Specifications
Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856) is a mouse monoclonal antibody and is validated for use in IHC-P, WB in human samples.
Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856) specifically detects HLA DR + DP + DQ (UniProt ID: P04440; Molecular weight: 26kDa) and is sold in 500µL selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856) has high sensitivity and specificity.
Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856) has been cited over 24 times in peer reviewed journals and is trusted by the scientific community.
Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856) has 4 independent reviews from customers.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

An alpha chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the beta chain HLA-DRB, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell receptor (TCR) on HLA-DR-restricted CD4-positive T cells. This guides antigen-specific T-helper effector functions, both antibody-mediated immune response and macrophage activation, to ultimately eliminate the infectious agents and transformed cells (PubMed : 15265931, PubMed : 15322540, PubMed : 17334368, PubMed : 22327072, PubMed : 24190431, PubMed : 27591323, PubMed : 29884618, PubMed : 31495665, PubMed : 8145819, PubMed : 9075930). Typically presents extracellular peptide antigens of 10 to 30 amino acids that arise from proteolysis of endocytosed antigens in lysosomes (PubMed : 8145819). In the tumor microenvironment, presents antigenic peptides that are primarily generated in tumor-resident APCs likely via phagocytosis of apoptotic tumor cells or macropinocytosis of secreted tumor proteins (PubMed : 31495665). Presents peptides derived from intracellular proteins that are trapped in autolysosomes after macroautophagy, a mechanism especially relevant for T cell selection in the thymus and central immune tolerance (PubMed : 17182262, PubMed : 23783831). The selection of the immunodominant epitopes follows two processing modes : 'bind first, cut/trim later' for pathogen-derived antigenic peptides and 'cut first, bind later' for autoantigens/self-peptides (PubMed : 25413013). The anchor residue at position 1 of the peptide N-terminus, usually a large hydrophobic residue, is essential for high affinity interaction with MHCII molecules (PubMed : 8145819).
See full target information HLA-DRA
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com