Anti-HLA E antibody [MEM-E/02] (ab2216) is a mouse monoclonal antibody that is used to detect HLA E in Western Blot, Flow Cytometry, IHC-P. Suitable for Human samples.
- Specificity confirmed with HLA E knockout cell line validation
Images
![Western blot - Anti-HLA E antibody [MEM-E/02] (AB2216), expandable thumbnail](https://content.abcam.com/products/images/hla-e-antibody-mem-e-02-ab2216--western-blot-img155851.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HLA E antibody [MEM-E/02] (AB2216)")
![Western blot - Anti-HLA E antibody [MEM-E/02] (AB2216), expandable thumbnail](https://content.abcam.com/products/images/hla-e-antibody-mem-e-02-ab2216--western-blot-img151515.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HLA E antibody [MEM-E/02] (AB2216)")
![Flow Cytometry - Anti-HLA E antibody [MEM-E/02] (AB2216), expandable thumbnail](https://content.abcam.com/products/images/hla-e-antibody-mem-e-02-ab2216--flow-cytometry-img79458.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-HLA E antibody [MEM-E/02] (AB2216)")
![Western blot - Anti-HLA E antibody [MEM-E/02] (AB2216), expandable thumbnail](https://content.abcam.com/products/images/hla-e-antibody-mem-e-02-ab2216--western-blot-img147953.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HLA E antibody [MEM-E/02] (AB2216)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA E antibody [MEM-E/02] (AB2216), expandable thumbnail](https://content.abcam.com/products/images/hla-e-antibody-mem-e-02-ab2216--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img6618.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA E antibody [MEM-E/02] (AB2216)")
Publications
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- Cancer cell 40:1027-1043.e92022NKG2A and HLA-E define an alternative immune checkpoint axis in bladder cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBérengère Salomé,John P Sfakianos,Daniel Ranti,Jorge Daza,Christine Bieber,Andrew Charap,Christian Hammer,Romain Banchereau,Adam M Farkas,Dan Fu Ruan,Sudeh Izadmehr,Daniel Geanon,Geoffrey Kelly,Ronaldo M de Real,Brian Lee,Kristin G Beaumont,Sanjana Shroff,Yuanshuo A Wang,Ying-Chih Wang,Tin Htwe Thin,Monica Garcia-Barros,Everardo Hegewisch-Solloa,Emily M Mace,Li Wang,Timothy O'Donnell,Diego Chowell,Ruben Fernandez-Rodriguez,Mihaela Skobe,Nicole Taylor,Seunghee Kim-Schulze,Robert P Sebra,Doug Palmer,Eleanor Clancy-Thompson,Scott Hammond,Alice O Kamphorst,Karl-Johan Malmberg,Emanuela Marcenaro,Pedro Romero,Rachel Brody,Mathias Viard,Yuko Yuki,Maureen Martin,Mary Carrington,Reza Mehrazin,Peter Wiklund,Ira Mellman,Sanjeev Mariathasan,Jun Zhu,Matthew D Galsky,Nina Bhardwaj,Amir HorowitzPubMed 36099881
- Cellular & molecular immunology 19:527-5392022Downregulated cytotoxic CD8 T-cell identifies with the NKG2A-soluble HLA-E axis as a predictive biomarker and potential therapeutic target in keloids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeng Xu,Zhu Zhu,Jian Hu,Jiawei Sun,Yan Wo,Xianshu Wang,Hongzhi Zou,Bin Li,Yixin ZhangPubMed 35039632
- The Journal of international medical research 49:30006052110472782021Lesion human leukocyte antigen-E is associated with favourable prognosis for patients with oesophageal squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYong-Fu Xu,Xue-Feng Du,Zhen-Yu Li,Zhe-Ping Fang,Fa-Biao ZhangPubMed 34617814
- International journal of molecular sciences 22:2021NK Cells Lose Their Cytotoxicity Function against Cancer Stem Cell-Rich Radiotherapy-Resistant Breast Cancer Cell Populations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHana Jin,Hye Jung KimPubMed 34502547
- Clinical cancer research : an official journal of the American Association for Cancer Research 27:807-8182020Effects of HER Family-targeting Tyrosine Kinase Inhibitors on Antibody-dependent Cell-mediated Cytotoxicity in HER2-expressing Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDenis M Collins,Stephen F Madden,Nicola Gaynor,Dalal AlSultan,Marion Le Gal,Alex J Eustace,Kathy A Gately,Clare Hughes,Anthony M Davies,Thamir Mahgoub,Jo Ballot,Sinead Toomey,Darran P O'Connor,William M Gallagher,Frankie A Holmes,Virginia Espina,Lance Liotta,Bryan T Hennessy,Kenneth J O'Byrne,Max Hasmann,Birgit Bossenmaier,Norma O'Donovan,John CrownPubMed 33122343
- HLA 96:197-2012020Human-porcine MHC-I homology allows for antibody cross-reactivity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicole Forneris,Heather Levy,Christopher BurlakPubMed 32452158
- Immunology letters 162:94-1002014Inhibition of ERK and proliferation in NK cell lines by soluble HLA-E released from Japanese encephalitis virus infected cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShwetank et. al.PubMed 25086398
- BMC cancer 14:4862014Prognostic value of HLA class I, HLA-E, HLA-G and Tregs in rectal cancer: a retrospective cohort study.
- British journal of cancer 111:532-82014Immunological subtypes in breast cancer are prognostic for invasive ductal but not for invasive lobular breast carcinoma.
- PloS one 8:e791972013Infection of human endothelial cells by Japanese encephalitis virus: increased expression and release of soluble HLA-E.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human HLA-E. Database link P13747
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg for 106 Cells | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5-10 µg/mL | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Non-classical major histocompatibility class Ib molecule involved in immune self-nonself discrimination. In complex with B2M/beta-2-microglobulin binds nonamer self-peptides derived from the signal sequence of classical MHC class Ia molecules (VL9 peptides - VMAPRT[V/L][L/V/I/F]L) (PubMed:18083576, PubMed:18339401, PubMed:35705051, PubMed:37264229, PubMed:9754572). Peptide-bound HLA-E-B2M heterotrimeric complex primarily functions as a ligand for natural killer (NK) cell inhibitory receptor KLRD1-KLRC1, enabling NK cells to monitor the expression of other MHC class I molecules in healthy cells and to tolerate self (PubMed:17179229, PubMed:18083576, PubMed:37264229, PubMed:9486650, PubMed:9754572). Upon cellular stress, preferentially binds signal sequence-derived peptides from stress-induced chaperones and is no longer recognized by NK cell inhibitory receptor KLRD1-KLRC1, resulting in impaired protection from NK cells (PubMed:12461076). Binds signal sequence-derived peptides from non-classical MHC class Ib HLA-G molecules and acts as a ligand for NK cell activating receptor KLRD1-KLRC2, likely playing a role in the generation and effector functions of adaptive NK cells and in maternal-fetal tolerance during pregnancy (PubMed:30134159, PubMed:37264229, PubMed:9754572). Besides self-peptides, can also bind and present pathogen-derived peptides conformationally similar to VL9 peptides to alpha-beta T cell receptor (TCR) on unconventional CD8-positive cytotoxic T cells, ultimately triggering antimicrobial immune response (PubMed:16474394, PubMed:20195504, PubMed:30087334, PubMed:34228645). Presents HIV gag peptides (immunodominant KAFSPEVIPMF and subdominant KALGPAATL epitopes) predominantly to CD8-positive T cell clones expressing a TRAV17-containing TCR, triggering HLA-E-restricted T cell responses (PubMed:34228645). Presents mycobacterial peptides to HLA-E-restricted CD8-positive T cells eliciting both cytotoxic and immunoregulatory functions (PubMed:20195504, PubMed:35705051). (Microbial infection) Viruses like human cytomegalovirus have evolved an escape mechanism whereby virus-induced down-regulation of host MHC class I molecules is coupled to the binding of viral peptides to HLA-E, restoring HLA-E expression and inducing HLA-E-dependent NK cell immune tolerance to infected cells. (Microbial infection) May bind HIV-1 gag/Capsid protein p24-derived peptide (AISPRTLNA) on infected cells and may inhibit NK cell cytotoxicity, a mechanism that allows HIV-1 to escape immune recognition. (Microbial infection) Upon SARS-CoV-2 infection, may contribute to functional exhaustion of cytotoxic NK cells and CD8-positive T cells (PubMed:32859121). Binds SARS-CoV-2 S/Spike protein S1-derived peptide (LQPRTFLL) expressed on the surface of lung epithelial cells, inducing NK cell exhaustion and dampening of antiviral immune surveillance (PubMed:32859121).
Alternative names
HLA-6.2, HLAE, HLA-E, MHC class I antigen E
Recommended products
Anti-HLA E antibody [MEM-E/02] (ab2216) is a mouse monoclonal antibody that is used to detect HLA E in Western Blot, Flow Cytometry, IHC-P. Suitable for Human samples.
- Specificity confirmed with HLA E knockout cell line validation
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human HLA-E. Database link P13747
- Clone number
- MEM-E/02
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody reacts with the denaturated heavy chain of human HLA-E. It does not cross-react with HLA-A, -B, -C or -G. Specifity of the antibody was confirmed on HLA-G/HLA-E Workshop(Victoria 2002).
- Light chain type
- unknown
- Concentration
- Purification notes
Purified from TCS. Purity >95% by SDS-PAGE.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-HLA E antibody [MEM-E/02] (ab2216) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Human samples.
What is the molecular weight of HLA E?
Anti-HLA E [MEM-E/02] (ab2216) specifically detects a band for HLA E (UniProt: P13747) at a molecular weight of 40kDa.
Trusted by the scientific community
Anti-HLA E [MEM-E/02] (ab2216) was first used in a scientific publication in 2002 and has been cited over 20 times in peer-reviewed journals.
Reviewed by scientists
Anti-HLA E [MEM-E/02] (ab2216) has over 5 independent reviews from customers.
Specificity confirmed
The specificity of Anti-HLA E antibody [MEM-E/02] (ab2216) has been confirmed by Western blot testing in HLA-E Knockout A549 cell line, Human HLA-E (HLA E) knockout HEK-293T cell line ab267231.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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5 product images
![Western blot - Anti-HLA E antibody [MEM-E/02] (ab2216), expandable thumbnail](https://content.abcam.com/products/images/hla-e-antibody-mem-e-02-ab2216--western-blot-img155851.jpg "Western blot - Anti-HLA E antibody [MEM-E/02] (ab2216)")
Western blot - Anti-HLA E antibody [MEM-E/02] (ab2216)
Lanes 1-4: Merged signal (red and green). Green - ab2216 observed at 40 kDa. Red - loading control Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 observed at 36 kDa.ab2216 Anti-HLA E antibody [MEM-E/02] was shown to specifically react with HLA E in wild-type A549 cells. Loss of signal was observed when knockout cell line Human HLA-E (HLA E) knockout A549 cell line ab267080 (knockout cell lysate Human HLA-E (HLA E) knockout A549 cell lysate ab258452) was used. Wild-type and HLA E knockout samples were subjected to SDS-PAGE. ab2216 and Anti-GAPDH antibody[EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) were incubated at room temperature for 2.5 hours at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-HLA E antibody [MEM-E/02] (ab2216) at 1/500 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: HLA-E knockout A549 cell lysate at 20 µg
Lane 2: Western blot - Human HLA-E (HLA E) knockout A549 cell line (Human HLA-E (HLA E) knockout A549 cell line ab267080)
Lane 3: THP-1 cell lysate at 20 µg
Lane 4: Jurkat cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/10000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
![Western blot - Anti-HLA E antibody [MEM-E/02] (ab2216), expandable thumbnail](https://content.abcam.com/products/images/hla-e-antibody-mem-e-02-ab2216--western-blot-img151515.jpg "Western blot - Anti-HLA E antibody [MEM-E/02] (ab2216)")
Western blot - Anti-HLA E antibody [MEM-E/02] (ab2216)
Lanes 1-4: Merged signal (red and green). Green - ab2216 observed at 40 kDa. Red - loading control Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 observed at 36 kDa.ab2216 Anti-HLA E antibody [MEM-E/02] was shown to specifically react with HLA E in wild-type A549 cells. Loss of signal was observed when knockout cell line Human HLA-E (HLA E) knockout A549 cell line ab267081 (knockout cell lysate Human HLA-E (HLA E) knockout A549 cell lysate ab258453) was used. Wild-type and HLA E knockout samples were subjected to SDS-PAGE. ab2216 and Anti-GAPDH antibody[EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) were incubated at room temperature for 2.5 hours at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-HLA E antibody [MEM-E/02] (ab2216) at 1/1000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: HLA-E knockout A549 cell lysate at 20 µg
Lane 2: Western blot - Human HLA-E (HLA E) knockout A549 cell line (Human HLA-E (HLA E) knockout A549 cell line ab267081)
Lane 3: THP-1 cell lysate at 20 µg
Lane 4: Jurkat cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/10000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
![Flow Cytometry - Anti-HLA E antibody [MEM-E/02] (ab2216), expandable thumbnail](https://content.abcam.com/products/images/hla-e-antibody-mem-e-02-ab2216--flow-cytometry-img79458.jpg "Flow Cytometry - Anti-HLA E antibody [MEM-E/02] (ab2216)")
Flow Cytometry - Anti-HLA E antibody [MEM-E/02] (ab2216)
Overlay histogram showing HL60 cells stained with ab2216 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2216, 1 µg/1x106 cells for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
![Western blot - Anti-HLA E antibody [MEM-E/02] (ab2216), expandable thumbnail](https://content.abcam.com/products/images/hla-e-antibody-mem-e-02-ab2216--western-blot-img147953.jpg "Western blot - Anti-HLA E antibody [MEM-E/02] (ab2216)")
Western blot - Anti-HLA E antibody [MEM-E/02] (ab2216)
Lanes 1-3: Merged signal (red and green). Green - ab2216 observed at 40 kDa. Red - loading control Anti-beta Tubulin antibody [EP1331Y] - Loading Control ab52901 observed at kDa.ab2216 Anti-HLA E antibody [MEM-E/02] was shown to specifically react with HLA E in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human HLA-E (HLA E) knockout HEK-293T cell line ab267231 (knockout cell lysate Human HLA-E (HLA E) knockout HEK-293T cell lysate ab258454) was used. Wild-type and HLA E knockout samples were subjected to SDS-PAGE. ab2216 and Anti-beta Tubulin [EP1331Y] - Microtubule Marker (Anti-beta Tubulin antibody [EP1331Y] - Loading Control ab52901) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-HLA E antibody [MEM-E/02] (ab2216) at 1/500 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: HLA-E knockout HEK-293T cell lysate at 20 µg
Lane 2: Western blot - Human HLA-E (HLA E) knockout HEK-293T cell line (Human HLA-E (HLA E) knockout HEK-293T cell line ab267231)
Lane 3: THP-1 cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA E antibody [MEM-E/02] (ab2216), expandable thumbnail](https://content.abcam.com/products/images/hla-e-antibody-mem-e-02-ab2216--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img6618.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA E antibody [MEM-E/02] (ab2216)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA E antibody [MEM-E/02] (ab2216)
IHC image of ab2216 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2216, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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