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Mouse Recombinant Monoclonal HLA F antibody. Suitable for WB, I-ELISA and reacts with Transfected cell lysate - Human, Human, Recombinant fragment - Human samples.

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Images

Western blot - Anti-HLA F antibody [3D11] (AB314767), expandable thumbnail
  • Western blot - Anti-HLA F antibody [3D11] (AB314767), expandable thumbnail

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBI-ELISAFlow CytIHC-P
Human
Tested
Expected
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Recombinant fragment - Human
Not recommended
Tested
Not recommended
Not recommended
Transfected cell lysate - Human
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Transfected cell lysate - Human

Dilution info
1/1000
Notes

-

Species

Human

Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant fragment - Human

Dilution info
2000 ng/mL
Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate - Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Transfected cell lysate - Human, Mouse, Rat, Recombinant fragment - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Transfected cell lysate - Human, Recombinant fragment - Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Non-classical major histocompatibility class Ib molecule postulated to play a role in immune surveillance, immune tolerance and inflammation. Functions in two forms, as a heterotrimeric complex with B2M/beta-2 microglobulin and a peptide (peptide-bound HLA-F-B2M) and as an open conformer (OC) devoid of peptide and B2M (peptide-free OC). In complex with B2M, presents non-canonical self-peptides carrying post-translational modifications, particularly phosphorylated self-peptides. Peptide-bound HLA-F-B2M acts as a ligand for LILRB1 inhibitory receptor, a major player in maternal-fetal tolerance. Peptide-free OC acts as a ligand for KIR3DS1 and KIR3DL2 receptors (PubMed:28636952). Upon interaction with activating KIR3DS1 receptor on NK cells, triggers NK cell degranulation and anti-viral cytokine production (PubMed:27455421). Through interaction with KIR3DL2 receptor, inhibits NK and T cell effector functions (PubMed:24018270). May interact with other MHC class I OCs to cross-present exogenous viral, tumor or minor histompatibility antigens to cytotoxic CD8+ T cells, triggering effector and memory responses (PubMed:23851683). May play a role in inflammatory responses in the peripheral nervous system. Through interaction with KIR3DL2, may protect motor neurons from astrocyte-induced toxicity (PubMed:26928464).

Alternative names

Recommended products

Mouse Recombinant Monoclonal HLA F antibody. Suitable for WB, I-ELISA and reacts with Transfected cell lysate - Human, Human, Recombinant fragment - Human samples.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

3D11

Purification technique

Affinity purification Protein A

Specificity

This antibody does not cross-react with human HLA A, HLA B and HLA C.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-HLA F antibody [3D11] (ab314767), expandable thumbnail

    Western blot - Anti-HLA F antibody [3D11] (ab314767)

    This antibody does not cross-react with human HLA A, HLA B and HLA C.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-HLA F antibody [3D11] (ab314767) at 1/1000 dilution

    Lane 1: 293T cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

    Lane 2: 293T cells transfected with a human HLA F expression vector containing a His-tag, whole cell lysate at 20 µg

    Lane 3: 293T cells transfected with a human HLA A expression vector containing a His-tag, whole cell lysate at 20 µg

    Lane 4: 293T cells transfected with a human HLA C expression vector containing a His-tag, whole cell lysate at 20 µg

    Lane 5: 293T cells transfected with a human HLA B expression vector containing a His-tag, whole cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Observed band size: 39 kDa, 36 kDa

    Exposure time: 10s

  • Western blot - Anti-HLA F antibody [3D11] (ab314767), expandable thumbnail

    Western blot - Anti-HLA F antibody [3D11] (ab314767)

    Low expression: skeletal muscle, kidney.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-HLA F antibody [3D11] (ab314767) at 1/1000 dilution

    Lane 1: Human tonsil tissue lysate at 20 µg

    Lane 2: Human lymphma tissue lysate at 20 µg

    Lane 3: Human skeletal muscle tissue lysate at 20 µg

    Lane 4: Human kidney tissue lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Observed band size: 39 kDa, 36 kDa

    Exposure time: 81s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com