AB126624

Anti-HLA F antibody [EPR6803]

Name: Anti-HLA F antibody [EPR6803] (ab126624) | Abcam
Brand: Abcam Limited
SKU: AB126624
Price: 490 USD
Availability: InStock
Rating: 4 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(4 Publications)

Rabbit Recombinant Monoclonal HLA F antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

HLA-5.4, HLAF, HLA-F, CDA12, HLA F antigen, Leukocyte antigen F, MHC class I antigen F

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA F antibody [EPR6803] (AB126624)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA F antibody [EPR6803] (AB126624)

ab126624 at a 1/250 dilution staining HLA F in paraffin-embedded Human tonsil tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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Western blot - Anti-HLA F antibody [EPR6803] (AB126624)

All lanes:

Western blot - Anti-HLA F antibody [EPR6803] (ab126624) at 1/1000 dilution

Lane 1:

HT1376 cell lysate at 10 µg

Lane 2:

Human tonsil lysate at 10 µg

Lane 3:

JAR cell lysate at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 39 kDa

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OI-RD Scanning - Anti-HLA F antibody [EPR6803] (AB126624)

OI-RD Scanning

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OI-RD Scanning - Anti-HLA F antibody [EPR6803] (AB126624)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-HLA F antibody [EPR6803] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6803

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Non-classical major histocompatibility class Ib molecule postulated to play a role in immune surveillance, immune tolerance and inflammation. Functions in two forms, as a heterotrimeric complex with B2M/beta-2 microglobulin and a peptide (peptide-bound HLA-F-B2M) and as an open conformer (OC) devoid of peptide and B2M (peptide-free OC). In complex with B2M, presents non-canonical self-peptides carrying post-translational modifications, particularly phosphorylated self-peptides. Peptide-bound HLA-F-B2M acts as a ligand for LILRB1 inhibitory receptor, a major player in maternal-fetal tolerance. Peptide-free OC acts as a ligand for KIR3DS1 and KIR3DL2 receptors (PubMed : 28636952). Upon interaction with activating KIR3DS1 receptor on NK cells, triggers NK cell degranulation and anti-viral cytokine production (PubMed : 27455421). Through interaction with KIR3DL2 receptor, inhibits NK and T cell effector functions (PubMed : 24018270). May interact with other MHC class I OCs to cross-present exogenous viral, tumor or minor histompatibility antigens to cytotoxic CD8+ T cells, triggering effector and memory responses (PubMed : 23851683). May play a role in inflammatory responses in the peripheral nervous system. Through interaction with KIR3DL2, may protect motor neurons from astrocyte-induced toxicity (PubMed : 26928464).

See full target information HLA-F

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1234577 PubMed37854606

2023

Single-cell profiling reveals immune disturbances landscape and HLA-F-mediated immune tolerance at the maternal-fetal interface in preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Fangyuan Luo,Fulin Liu,Yingzhe Guo,Wenming Xu,Yilin Li,Jun Yi,Thierry Fournier,Séverine Degrelle,Hedia Zitouni,Isabelle Hernandez,Xinghui Liu,Yu Huang,Jun Yue

Cells 12: PubMed36672208

2023

Silencing of circCRIM1 Drives IGF2BP1-Mediated NSCLC Immune Evasion.

Applications

Unspecified application

Species

Unspecified reactive species

Wenbei Peng,Linlin Ye,Qianqian Xue,Xiaoshan Wei,Zihao Wang,Xuan Xiang,Siyu Zhang,Pei Zhang,Haolei Wang,Qiong Zhou

Analytical cellular pathology (Amsterdam) 2018:7691704 PubMed30510890

2018

High HLA-F Expression Is a Poor Prognosis Factor in Patients with Nasopharyngeal Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Wu,Haihua Yang,Shenpeng Ying,Hongsheng Lu,Wei Wang,Jiaming Lv,Huacai Xiong,Wei Hu

American journal of reproductive immunology (New Y 78: PubMed28440588

2017

Endometrial immune markers are potential predictors of normal fertility and pregnancy after in vitro fertilization.

Applications

Unspecified application

Species

Unspecified reactive species

Louise Kofod,Anette Lindhard,Michael Bzorek,Jens Ole Eriksen,Lise Grupe Larsen,Thomas Vauvert F Hviid

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HLA F antibody [EPR6803]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA F antibody [EPR6803] (AB126624)

Western blot - Anti-HLA F antibody [EPR6803] (AB126624)

OI-RD Scanning - Anti-HLA F antibody [EPR6803] (AB126624)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (4)

Product promise