Anti-HLA G antibody [EPR23298-97] (ab283260) is a rabbit monoclonal antibody that is used to detect HLA G in Western Blot, IHC-P, ICC/IF. Suitable for Human samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [EPR23298-97] (AB283260), expandable thumbnail](https://content.abcam.com/products/images/hla-g-antibody-epr23298-97-ab283260--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img181331.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [EPR23298-97] (AB283260)")
![Western blot - Anti-HLA G antibody [EPR23298-97] (AB283260), expandable thumbnail](https://content.abcam.com/products/images/hla-g-antibody-epr23298-97-ab283260--western-blot-img166147.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HLA G antibody [EPR23298-97] (AB283260)")
![Immunocytochemistry/ Immunofluorescence - Anti-HLA G antibody [EPR23298-97] (AB283260), expandable thumbnail](https://content.abcam.com/products/images/hla-g-antibody-epr23298-97-ab283260--immunocytochemistry-immunofluorescence-img172513.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-HLA G antibody [EPR23298-97] (AB283260)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [EPR23298-97] (AB283260), expandable thumbnail](https://content.abcam.com/products/images/hla-g-antibody-epr23298-97-ab283260--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img170318.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [EPR23298-97] (AB283260)")
![Western blot - Anti-HLA G antibody [EPR23298-97] (AB283260), expandable thumbnail](https://content.abcam.com/products/images/hla-g-antibody-epr23298-97-ab283260--western-blot-img171205.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HLA G antibody [EPR23298-97] (AB283260)")
Publications
Filter by
- Journal of cell science 137:2023Trophoblast organoids with physiological polarity model placental structure and function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiheng Yang,Pengfei Liang,Huanghe Yang,Carolyn B CoynePubMed 37676312
- Molecular therapy. Methods & clinical development 29:227-2352023AAV-mediated expression of HLA-G for the prevention of experimental ocular graft vs. host disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJacob P Nilles,Darby Roberts,Jacklyn H Salmon,Liujiang Song,Carly O'Dea,Lindsay T Marjoram,Jacquelyn J Bower,Matthew L Hirsch,Brian C GilgerPubMed 37090476
- Cell discovery 8:1372022Structural insights into the covalent regulation of PAPP-A activity by proMBP and STC2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQihang Zhong,Honglei Chu,Guopeng Wang,Cheng Zhang,Rong Li,Fusheng Guo,Xinlu Meng,Xiaoguang Lei,Youli Zhou,Ruobing Ren,Lin Tao,Ningning Li,Ning Gao,Yuan Wei,Jie Qiao,Jing HangPubMed 36550107
- BMC biology 20:2762022An IGF1-expressing endometrial stromal cell population is associated with human decidualization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia-Wei Shi,Zhen-Zhen Lai,Hui-Li Yang,Wen-Jie Zhou,Xiao-Ya Zhao,Feng Xie,Song-Ping Liu,Wei-Dong Chen,Tao Zhang,Jiang-Feng Ye,Xiang-Yu Zhou,Ming-Qing LiPubMed 36482461
- Cellular & molecular immunology 19:527-5392022Downregulated cytotoxic CD8 T-cell identifies with the NKG2A-soluble HLA-E axis as a predictive biomarker and potential therapeutic target in keloids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeng Xu,Zhu Zhu,Jian Hu,Jiawei Sun,Yan Wo,Xianshu Wang,Hongzhi Zou,Bin Li,Yixin ZhangPubMed 35039632
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Isoform 1. Non-classical major histocompatibility class Ib molecule involved in immune regulatory processes at the maternal-fetal interface (PubMed:19304799, PubMed:23184984, PubMed:29262349). In complex with B2M/beta-2 microglobulin binds a limited repertoire of nonamer self-peptides derived from intracellular proteins including histones and ribosomal proteins (PubMed:7584149, PubMed:8805247). Peptide-bound HLA-G-B2M complex acts as a ligand for inhibitory/activating KIR2DL4, LILRB1 and LILRB2 receptors on uterine immune cells to promote fetal development while maintaining maternal-fetal tolerance (PubMed:16366734, PubMed:19304799, PubMed:20448110, PubMed:23184984, PubMed:27859042, PubMed:29262349). Upon interaction with KIR2DL4 and LILRB1 receptors on decidual NK cells, it triggers NK cell senescence-associated secretory phenotype as a molecular switch to promote vascular remodeling and fetal growth in early pregnancy (PubMed:16366734, PubMed:19304799, PubMed:23184984, PubMed:29262349). Through interaction with KIR2DL4 receptor on decidual macrophages induces pro-inflammatory cytokine production mainly associated with tissue remodeling (PubMed:19304799). Through interaction with LILRB2 receptor triggers differentiation of type 1 regulatory T cells and myeloid-derived suppressor cells, both of which actively maintain maternal-fetal tolerance (PubMed:20448110, PubMed:27859042). May play a role in balancing tolerance and antiviral-immunity at maternal-fetal interface by keeping in check the effector functions of NK, CD8+ T cells and B cells (PubMed:10190900, PubMed:11290782, PubMed:24453251). Reprograms B cells toward an immune suppressive phenotype via LILRB1 (PubMed:24453251). May induce immune activation/suppression via intercellular membrane transfer (trogocytosis), likely enabling interaction with KIR2DL4, which resides mostly in endosomes (PubMed:20179272, PubMed:26460007). Through interaction with the inhibitory receptor CD160 on endothelial cells may control angiogenesis in immune privileged sites (PubMed:16809620). Isoform 2. Likely does not bind B2M and presents peptides. Negatively regulates NK cell- and CD8+ T cell-mediated cytotoxicity (PubMed:11290782). Isoform 3. Likely does not bind B2M and presents peptides. Negatively regulates NK cell- and CD8+ T cell-mediated cytotoxicity (PubMed:11290782). Isoform 4. Likely does not bind B2M and presents peptides. Negatively regulates NK cell- and CD8+ T cell-mediated cytotoxicity (PubMed:11290782). Isoform 5. Non-classical major histocompatibility class Ib molecule involved in immune regulatory processes at the maternal-fetal interface (PubMed:19304799, PubMed:23184984, PubMed:29262349). In complex with B2M/beta-2 microglobulin binds a limited repertoire of nonamer self-peptides derived from intracellular proteins including histones and ribosomal proteins (PubMed:7584149, PubMed:8805247). Peptide-bound HLA-G-B2M complex acts as a ligand for inhibitory/activating KIR2DL4, LILRB1 and LILRB2 receptors on uterine immune cells to promote fetal development while maintaining maternal-fetal tolerance (PubMed:16366734, PubMed:19304799, PubMed:20448110, PubMed:23184984, PubMed:29262349). Upon interaction with KIR2DL4 and LILRB1 receptors on decidual NK cells, it triggers NK cell senescence-associated secretory phenotype as a molecular switch to promote vascular remodeling and fetal growth in early pregnancy (PubMed:16366734, PubMed:19304799, PubMed:23184984, PubMed:29262349). Through interaction with KIR2DL4 receptor on decidual macrophages induces pro-inflammatory cytokine production mainly associated with tissue remodeling (PubMed:19304799). Through interaction with LILRB2 receptor triggers differentiation of type 1 regulatory T cells and myeloid-derived suppressor cells, both of which actively maintain maternal-fetal tolerance (PubMed:20448110). Reprograms B cells toward an immune suppressive phenotype via LILRB1 (PubMed:24453251). Isoform 6. Likely does not bind B2M and presents peptides. Isoform 7. Likely does not bind B2M and presents peptides.
Alternative names
HLA-6.0, HLAG, HLA-G, HLA G antigen, MHC class I antigen G
Recommended products
Anti-HLA G antibody [EPR23298-97] (ab283260) is a rabbit monoclonal antibody that is used to detect HLA G in Western Blot, IHC-P, ICC/IF. Suitable for Human samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR23298-97
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-HLA G antibody [EPR23298-97] (ab283260) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of HLA G?
Anti-HLA G [EPR23298-97] (ab283260) specifically detects a band for HLA G (UniProt: P17693) at a molecular weight of 38kDa.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR23298-97] also available for your convenience:
ab283260, Carrier free - Anti-HLA G antibody [EPR23298-97] - BSA and Azide free ab283278, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-HLA G antibody [EPR23298-97] ab300056
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [EPR23298-97] (ab283260), expandable thumbnail](https://content.abcam.com/products/images/hla-g-antibody-epr23298-97-ab283260--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img181331.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [EPR23298-97] (ab283260)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [EPR23298-97] (ab283260)
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling HLA G with ab283260 at 1/20000 (0.026 μg/mL) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on extravillous trophoblasts (EVTs) in human placenta (PMID:18538632, PMID:29249463, PMID:29249436). The section was incubated with ab283260 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Western blot - Anti-HLA G antibody [EPR23298-97] (ab283260), expandable thumbnail](https://content.abcam.com/products/images/hla-g-antibody-epr23298-97-ab283260--western-blot-img166147.jpg "Western blot - Anti-HLA G antibody [EPR23298-97] (ab283260)")
Western blot - Anti-HLA G antibody [EPR23298-97] (ab283260)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: HuT-78 (PMID:2249951).
Exposure time: 48 seconds.All lanes: Western blot - Anti-HLA G antibody [EPR23298-97] (ab283260) at 1/1000 dilution
Lane 1: JEG-3 (human placenta choriocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: HuT-78 (human sezary syndrome cutaneous T lymphocyte) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 38 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-HLA G antibody [EPR23298-97] (ab283260), expandable thumbnail](https://content.abcam.com/products/images/hla-g-antibody-epr23298-97-ab283260--immunocytochemistry-immunofluorescence-img172513.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HLA G antibody [EPR23298-97] (ab283260)")
Immunocytochemistry/ Immunofluorescence - Anti-HLA G antibody [EPR23298-97] (ab283260)
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized JEG-3 cells labelling HLA G with ab283260 at 1/100 (5.18 μg/mL) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 μg/mL) dilution (Green). Confocal image showing cytoplasmic and membranous staining in JEG-3 cell line. Negative control: HuT-78 (PMID: 2249951). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 μg/mL) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/mL) dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [EPR23298-97] (ab283260), expandable thumbnail](https://content.abcam.com/products/images/hla-g-antibody-epr23298-97-ab283260--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img170318.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [EPR23298-97] (ab283260)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [EPR23298-97] (ab283260)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling HLA G with ab283260 at 1/20000 (0.026 μg/mL) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human breast carcinoma. The section was incubated with ab283260 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Western blot - Anti-HLA G antibody [EPR23298-97] (ab283260), expandable thumbnail](https://content.abcam.com/products/images/hla-g-antibody-epr23298-97-ab283260--western-blot-img171205.jpg "Western blot - Anti-HLA G antibody [EPR23298-97] (ab283260)")
Western blot - Anti-HLA G antibody [EPR23298-97] (ab283260)
Blocking and diluting buffer and concentration: 5% NFDM/TBSTNegative control: heart (PMID:22438923)
Exposure time: 26 seconds
All lanes: Western blot - Anti-HLA G antibody [EPR23298-97] (ab283260) at 1/1000 dilution
Lane 1: Human placenta tissue lysate at 20 µg
Lane 2: Human heart tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 38 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [EPR23298-97] (ab283260), expandable thumbnail](https://content.abcam.com/products/images/hla-g-antibody-epr23298-97-ab283260--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img167174.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [EPR23298-97] (ab283260)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [EPR23298-97] (ab283260)
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labelling HLA G with ab283260 at 1/20000 (0.026 μg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: no staining on human cardiac muscle (PMID:22438923). The section was incubated with ab283260 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [EPR23298-97] (ab283260), expandable thumbnail](https://content.abcam.com/products/images/hla-g-antibody-epr23298-97-ab283260--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img167174.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [EPR23298-97] (ab283260)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com