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AB190338

Anti-hlgB antibody

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(1 Publication)

Rabbit Polyclonal HLGB antibody. Suitable for WB and reacts with Recombinant full length protein - Staphylococcus aureus, Staphylococcus aureus samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Staphylococcus aureus subsp. aureus N315 hlgB.

View Alternative Names

SA2209, hlgB, Gamma-hemolysin component B, H-gamma-1, H-gamma-I

2 Images
Western blot - Anti-hlgB antibody (AB190338)
  • WB

Supplier Data

Western blot - Anti-hlgB antibody (AB190338)

All lanes:

Western blot - Anti-hlgB antibody (ab190338) at 0.5 µg/mL

All lanes:

Recombinant hlg B protein at 0.1 µg

Secondary

All lanes:

Anti-rabbit IgG-AP conjugate

Predicted band size: 37 kDa

false

Western blot - Anti-hlgB antibody (AB190338)
  • WB

Supplier Data

Western blot - Anti-hlgB antibody (AB190338)

All lanes:

Western blot - Anti-hlgB antibody (ab190338) at 0.5 µg/mL

Lane 1:

Culture supernatant of Staphylococcus aureus strain USA300 at 15 µL

Lane 2:

Culture supernatant of Staphylococcus aureus strain Newman at 15 µL

Lane 3:

Culture supernatant of Staphylococcus aureus strain NCTC 8325-4 at 15 µL

Lane 4:

Culture supernatant of Staphylococcus aureus strain exoprotein negative NCTC 8325-4 at 15 µL

Secondary

All lanes:

Anti-rabbit IgG-AP conjugate

Predicted band size: 37 kDa

Observed band size: <40 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Staphylococcus aureus

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Staphylococcus aureus subsp. aureus N315 hlgB.

P0A075

Specificity

Due to the high level of homology among the bi-component cytolysins, ab190338 demonstrates cross-reactivity to rHlg C, rLukF-PV and rLukS-PV . The immunogen contained a His-tag, therefore, proteins containing a His-epitope tag may also cross-react. This antibody is an IgG fraction of serum and therefore may have antibodies to other antigens including Staphylococcal antigens as a result of natural exposure and immune response.

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Reactivity data

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Product details

av190338 can be used for detection of gamma-hemolysin secreted by S. aureus.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The hlgB protein also known as gamma-hemolysin component B or leucotoxin B is part of the gamma-hemolysin complex. This protein is recognized for its role in membrane disruption consisting of two components that work together to form pores in target cell membranes. The hlgB protein has a molecular mass of approximately 34 kDa and is expressed primarily by Staphylococcus aureus. It contributes to the bacterium’s virulence by targeting and lysing host cells.
Biological function summary

Gamma-hemolysin component B and its partner proteins form a two-component system. HlgB pairs with another component hlgA or hlgC to interact with the phospholipids in host cell membranes leading to the formation of transmembrane pores. This activity disrupts cellular homeostasis resulting in cell lysis. As a part of the hemolysin family hlgB contributes to the immune evasion strategies of bacteria by damaging host immune cells including leukocytes.

Pathways

HlgB is involved in pathways that enable bacterial immune evasion and pathogenesis. An important pathway it participates in is the induction of pore formation in leukocytes creating immune suppression. Related proteins in this pathway include other hemolysins like alpha-hemolysin that also contribute to pore formation and cytotoxic activities.

HlgB is significant in its relation to staphylococcal infections including skin infections and pneumonia. It represents a virulence factor that worsens disease severity by facilitating bacterial penetration and persistence in host tissues. The interaction with other proteins like PVL (panton-valentine leukocidin) further enhances its pathogenic potential contributing to severe outcomes in staphylococcal disease manifestations.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Toxin that seems to act by forming pores in the membrane of the cell. Has a hemolytic and a leucotoxic activity. Promotes host AMFR-mediated inflammation by mediating 'Lys-27'-linked ubiquitination of TAB3, TAK1-TAB3 complex formation and phosphorylation of TAK1/MAP3K7. In turn, activates host NF-kappa-B signaling pathway.
See full target information hlgB
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 9:2702 PubMed30006572

2018

ATP synthase F subunits recruited to centromeres by CENP-A are required for male meiosis.

Applications

Unspecified application

Species

Unspecified reactive species

Caitríona M Collins,Beatrice Malacrida,Colin Burke,Patrick A Kiely,Elaine M Dunleavy
View all publications
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