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AB17984

Anti-HLTF antibody

3

(2 Reviews)

|

(17 Publications)

Rabbit Polyclonal HLTF antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 17 publications. Immunogen corresponding to Synthetic Peptide within Human HLTF aa 600-700.

View Alternative Names

HIP116A, RNF80, SMARCA3, SNF2L3, ZBU1, HLTF, Helicase-like transcription factor, DNA-binding protein/plasminogen activator inhibitor 1 regulator, HIP116, RING finger protein 80, RING-type E3 ubiquitin transferase HLTF, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3, Sucrose nonfermenting protein 2-like 3

2 Images
Western blot - Anti-HLTF antibody (AB17984)
  • WB

Unknown

Western blot - Anti-HLTF antibody (AB17984)

Detection of SMARCA3 by western blot and immunoprecipitation.
Lane 1 : 20 μg of HeLa nuclear extract
Lane 2 : 50 μg of HeLa nuclear extract
LAne 3 : 1 mg of HeLa nuclear extract immunoprecipitated by anti-SMARCA3 (ab17984), used at 4 μg/mg of lysate.
Anti-SMARCA3 (ab17984) was used for western blot analysis at 1/5,000.
Detection : chemiluminescence with an exposure time of 4 minutes.

Detection of SMARCA3 by western blot and immunoprecipitation.
Lane 1 : 20 μg of HeLa nuclear extract
Lane 2 : 50 μg of HeLa nuclear extract
LAne 3 : 1 mg of HeLa nuclear extract immunoprecipitated by anti-SMARCA3 (ab17984), used at 4 μg/mg of lysate.
Anti-SMARCA3 (ab179

All lanes:

Western blot - Anti-HLTF antibody (ab17984)

Predicted band size: 113 kDa

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Western blot - Anti-HLTF antibody (AB17984)
  • WB

Unknown

Western blot - Anti-HLTF antibody (AB17984)

All lanes:

Western blot - Anti-HLTF antibody (ab17984) at 0.5 µg/mL

Lane 1:

NIH 3T3 whole cell lysate at 50 µg

Lane 2:

TCMK-1 whole cell lysate at 50 µg

Lane 3:

4T1 whole cell lysate at 50 µg

Lane 4:

CT26.WT whole cell lysate at 50 µg

Predicted band size: 113 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human HLTF aa 600-700. The exact immunogen used to generate this antibody is proprietary information.

Q14527

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/3000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/3000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Platypus": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibody was affinity purified using an epitope specific to SMARCA3 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA.
See full target information HLTF
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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:5789 PubMed38987539

2024

HLTF disrupts Cas9-DNA post-cleavage complexes to allow DNA break processing.

Applications

Unspecified application

Species

Unspecified reactive species

Giordano Reginato,Maria Rosaria Dello Stritto,Yanbo Wang,Jingzhou Hao,Raphael Pavani,Michael Schmitz,Swagata Halder,Vincent Morin,Elda Cannavo,Ilaria Ceppi,Stefan Braunshier,Ananya Acharya,Virginie Ropars,Jean-Baptiste Charbonnier,Martin Jinek,Andrè Nussenzweig,Taekjip Ha,Petr Cejka

Science (New York, N.Y.) 385:eado3867 PubMed38900911

2024

Structure and repair of replication-coupled DNA breaks.

Applications

Unspecified application

Species

Unspecified reactive species

Raphael Pavani,Veenu Tripathi,Kyle B Vrtis,Dali Zong,Raj Chari,Elsa Callen,Ajith V Pankajam,Gang Zhen,Gabriel Matos-Rodrigues,Jiajie Yang,Shuheng Wu,Giordano Reginato,Wei Wu,Petr Cejka,Johannes C Walter,André Nussenzweig

Biology direct 19:2 PubMed38163902

2024

DTX2 promotes glioma development via regulation of HLTF.

Applications

Unspecified application

Species

Unspecified reactive species

Ren Li,Yang Chen,Biao Yang,Ziao Li,Peize Li,Yu Chen,Jiayu Li,Jianhang He,Yongqiang Wu,Yanqi Sun,Xiaogang Wang,Xiaolong Guo,Wenju Zhang,Yuanli Zhao,Geng Guo

Nucleic acids research 51:12207-12223 PubMed37897354

2023

Human HELQ regulates DNA end resection at DNA double-strand breaks and stalled replication forks.

Applications

Unspecified application

Species

Unspecified reactive species

Yuqin Zhao,Kaiping Hou,Youhang Li,Shuailin Hao,Yu Liu,Yinan Na,Chao Li,Jian Cui,Xingzhi Xu,Xiaohua Wu,Hailong Wang

Life science alliance 5: PubMed35905994

2022

RFWD3 and translesion DNA polymerases contribute to PCNA modification-dependent DNA damage tolerance.

Applications

Unspecified application

Species

Unspecified reactive species

Rie Kanao,Hidehiko Kawai,Toshiyasu Taniguchi,Minoru Takata,Chikahide Masutani

Nature communications 13:66 PubMed35013187

2022

TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.

Applications

Unspecified application

Species

Unspecified reactive species

Roy Matkovic,Marina Morel,Sophie Lanciano,Pauline Larrous,Benjamin Martin,Fabienne Bejjani,Virginie Vauthier,Maike M K Hansen,Stéphane Emiliani,Gael Cristofari,Sarah Gallois-Montbrun,Florence Margottin-Goguet

PloS one 16:e0251132 PubMed34010296

2021

Helicase-like transcription factor-deletion from the tumor microenvironment in a cell line-derived xenograft model of colorectal cancer reprogrammed the human transcriptome-S-nitroso-proteome to promote inflammation and redirect metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca A Helmer,Raul Martinez-Zaguilan,Gurvinder Kaur,Lisa A Smith,Jannette M Dufour,Beverly S Chilton

Molecular cell 80:327-344.e8 PubMed32966758

2020

PCAF-Mediated Histone Acetylation Promotes Replication Fork Degradation by MRE11 and EXO1 in BRCA-Deficient Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jae Jin Kim,Seo Yun Lee,Ji-Hye Choi,Hyun Goo Woo,Blerta Xhemalce,Kyle M Miller

Nucleic acids research 46:11340-11356 PubMed30335157

2018

Regulation of HLTF-mediated PCNA polyubiquitination by RFC and PCNA monoubiquitination levels determines choice of damage tolerance pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuji Masuda,Satoshi Mitsuyuki,Rie Kanao,Asami Hishiki,Hiroshi Hashimoto,Chikahide Masutani

Cell host & microbe 24:447-460.e11 PubMed30122656

2018

High-Definition Analysis of Host Protein Stability during Human Cytomegalovirus Infection Reveals Antiviral Factors and Viral Evasion Mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Katie Nightingale,Kai-Min Lin,Benjamin J Ravenhill,Colin Davies,Luis Nobre,Ceri A Fielding,Eva Ruckova,Alice Fletcher-Etherington,Lior Soday,Hester Nichols,Daniel Sugrue,Eddie C Y Wang,Pablo Moreno,Yagnesh Umrania,Edward L Huttlin,Robin Antrobus,Andrew J Davison,Gavin W G Wilkinson,Richard J Stanton,Peter Tomasec,Michael P Weekes
View all publications
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