Anti-HLTF antibody [EPR14761] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLTF antibody [EPR14761] - BSA and Azide free (AB250652)

This data was developed using ab183042, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling HLTF with ab183042 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HLTF antibody [EPR14761] - BSA and Azide free (AB250652)

This data was developed using ab183042, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of Jurkat cells (-20°C Acetone-fixed) labeling HLTF with ab183042 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLTF antibody [EPR14761] - BSA and Azide free (AB250652)

This data was developed using ab183042, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human infiltrating duct carcinoma of breast tissue labeling HLTF with ab183042 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-HLTF antibody [EPR14761] - BSA and Azide free (AB250652)

This data was developed using ab183042, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HLTF antibody [EPR14761] (<a href='/en-us/products/primary-antibodies/hltf-antibody-epr14761-ab183042'>ab183042</a>) at 1/50000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

K562 lysate at 10 µg

Lane 3:

Jurkat lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 113 kDa,32 kDa

true

• WB

Lab

Western blot - Anti-HLTF antibody [EPR14761] - BSA and Azide free (AB250652)

This data was developed using ab183042, the same antibody clone in a different buffer formulation.

Lanes 1 - 3 : Merged signal (red and green). Green - ab183042 observed at 113 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab183042 was shown to specifically react with HLTF in wild-type HAP1 cells as signal was lost in HLTF knockout cells. Wild-type and HLTF knockout samples were subjected to SDS-PAGE. ab183042 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-HLTF antibody [EPR14761] (<a href='/en-us/products/primary-antibodies/hltf-antibody-epr14761-ab183042'>ab183042</a>) at 1 µg

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

HLTF knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HeLa whole cell lysate at 20 µg

Predicted band size: 113 kDa

Observed band size: 113 kDa

false