AB129092

Anti-HMBS/PBGD antibody [EPR8105]

RabMAb
Recombinant
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(8 Publications)

Rabbit Recombinant Monoclonal HMBS/PBGD antibody. Suitable for WB and reacts with Human samples. Cited in 8 publications.

View Alternative Names

PBGD, UPS, HMBS, Porphobilinogen deaminase, PBG-D, Hydroxymethylbilane synthase, Pre-uroporphyrinogen synthase

2 Images

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Western blot - Anti-HMBS/PBGD antibody [EPR8105] (AB129092)

All lanes:

Western blot - Anti-HMBS/PBGD antibody [EPR8105] (ab129092) at 1/1000 dilution

Lane 1:

HepG2 cell lysates at 10 µg

Lane 2:

HeLa cell lysates at 10 µg

Lane 3:

U937 cell lysates at 10 µg

Lane 4:

K562 cell lysates at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 39 kDa

Observed band size: 42-44 kDa

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OI-RD Scanning - Anti-HMBS/PBGD antibody [EPR8105] (AB129092)

OI-RD Scanning

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OI-RD Scanning - Anti-HMBS/PBGD antibody [EPR8105] (AB129092)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-HMBS/PBGD antibody [EPR8105] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8105

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

As part of the heme biosynthetic pathway, catalyzes the sequential polymerization of four molecules of porphobilinogen to form hydroxymethylbilane, also known as preuroporphyrinogen (PubMed : 18004775, PubMed : 18936296, PubMed : 19138865, PubMed : 23815679). Catalysis begins with the assembly of the dipyrromethane cofactor by the apoenzyme from two molecules of porphobilinogen or from preuroporphyrinogen. The covalently linked cofactor acts as a primer, around which the tetrapyrrole product is assembled. In the last step of catalysis, the product, preuroporphyrinogen, is released, leaving the cofactor bound to the holodeaminase intact (PubMed : 18936296).

See full target information HMBS

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Translational psychiatry 13:269 PubMed37491335

2023

A role of splenic heme biosynthesis pathway in the persistent prophylactic actions of arketamine in lipopolysaccharide-treated mice.

Applications

Unspecified application

Species

Unspecified reactive species

Li Ma,Long Wang,Youge Qu,Xiayun Wan,Kenji Hashimoto

NPJ Regenerative medicine 7:55 PubMed36151109

2022

ZO-2/Tjp2 suppresses Yap and Wwtr1/Taz-mediated hepatocyte to cholangiocyte transdifferentiation in the mouse liver.

Applications

Unspecified application

Species

Unspecified reactive species

Jianliang Xu,P Jaya Kausalya,Alicia Ghia Min Ong,Christine Meng Fan Goh,Safiah Mohamed Ali,Walter Hunziker

Science translational medicine 14:eabc0700 PubMed35020410

2022

Recombinant porphobilinogen deaminase targeted to the liver corrects enzymopenia in a mouse model of acute intermittent porphyria.

Applications

Unspecified application

Species

Unspecified reactive species

Karol M Córdoba,Irantzu Serrano-Mendioroz,Daniel Jericó,María Merino,Lei Jiang,Ana Sampedro,Manuel Alegre,Fernando Corrales,María J Garrido,Paolo G V Martini,José Luis Lanciego,Jesús Prieto,Pedro Berraondo,Antonio Fontanellas

Cell metabolism 33:199-210.e8 PubMed33152323

2020

Functional Genomics Identifies Metabolic Vulnerabilities in Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Douglas E Biancur,Kevin S Kapner,Keisuke Yamamoto,Robert S Banh,Jasper E Neggers,Albert S W Sohn,Warren Wu,Robert T Manguso,Adam Brown,David E Root,Andrew J Aguirre,Alec C Kimmelman

Molecular medicine reports 22:516-524 PubMed32377710

2020

A novel heterozygous mutation in the HMBS gene in a patient with acute intermittent porphyria and posterior reversible encephalopathy syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yang,Xiyun Chen,Huijuan Wu,Hua Peng,Wenjing Sun,Bin He,Zhengang Yuan

Acta pharmaceutica Sinica. B 9:937-951 PubMed31649844

2019

Synergistic antitumor activity of artesunate and HDAC inhibitors through elevating heme synthesis synergistic upregulation of ALAS1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Cai-Ping Chen,Kun Chen,Zhiqi Feng,Xiaoan Wen,Hongbin Sun

Journal of inherited metabolic disease 42:186-194 PubMed30740734

2019

Identification and characterization of 40 novel hydroxymethylbilane synthase mutations that cause acute intermittent porphyria.

Applications

Unspecified application

Species

Unspecified reactive species

Brenden Chen,Constanza Solis-Villa,Angelika L Erwin,Manisha Balwani,Irina Nazarenko,John D Phillips,Robert J Desnick,Makiko Yasuda

Nature medicine 24:1899-1909 PubMed30297912

2018

Systemic messenger RNA as an etiological treatment for acute intermittent porphyria.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Jiang,Pedro Berraondo,Daniel Jericó,Lin T Guey,Ana Sampedro,Andrea Frassetto,Kerry E Benenato,Kristine Burke,Eva Santamaría,Manuel Alegre,Álvaro Pejenaute,Mayur Kalariya,William Butcher,Ji-Sun Park,Xuling Zhu,Staci Sabnis,E Sathyajith Kumarasinghe,Timothy Salerno,Matthew Kenney,Christine M Lukacs,Matías A Ávila,Paolo G V Martini,Antonio Fontanellas

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HMBS/PBGD antibody [EPR8105]

Western blot - Anti-HMBS/PBGD antibody [EPR8105] (AB129092)

OI-RD Scanning - Anti-HMBS/PBGD antibody [EPR8105] (AB129092)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (8)

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