Rabbit Recombinant Monoclonal HMG4 antibody. Suitable for ICC/IF, IP, WB, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 8 publications.
Images
![Western blot - Anti-HMG4 antibody [EP2839Y] (AB75782), expandable thumbnail](https://content.abcam.com/products/images/hmg4-antibody-ep2839y-ab75782--western-blot-img66463.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HMG4 antibody [EP2839Y] (AB75782)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMG4 antibody [EP2839Y] (AB75782), expandable thumbnail](https://content.abcam.com/products/images/hmg4-antibody-ep2839y-ab75782--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131105.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMG4 antibody [EP2839Y] (AB75782)")
![Immunoprecipitation - Anti-HMG4 antibody [EP2839Y] (AB75782), expandable thumbnail](https://content.abcam.com/products/images/hmg4-antibody-ep2839y-ab75782--immunoprecipitation-img7796.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-HMG4 antibody [EP2839Y] (AB75782)")
![Immunocytochemistry/ Immunofluorescence - Anti-HMG4 antibody [EP2839Y] (AB75782), expandable thumbnail](https://content.abcam.com/products/images/hmg4-antibody-ep2839y-ab75782--immunocytochemistry-immunofluorescence-img68409.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-HMG4 antibody [EP2839Y] (AB75782)")
![Flow Cytometry (Intracellular) - Anti-HMG4 antibody [EP2839Y] (AB75782), expandable thumbnail](https://content.abcam.com/products/images/hmg4-antibody-ep2839y-ab75782--flow-cytometry-intracellular-img78736.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-HMG4 antibody [EP2839Y] (AB75782)")
Publications
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- The Prostate 83:440-4532022SOX9 and HMGB3 co-operatively transactivate NANOG and promote prostate cancer progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYunyi Xu,Miao Xu,Xinglan Li,Xin Weng,Zhengzheng Su,Mengni Zhang,Junya Tan,Hao Zeng,Xun Li,Ling Nie,Jing Gong,Ni Chen,Xueqin Chen,Qiao ZhouPubMed 36541373
- Cell death & disease 13:2632022HMGB3 promotes PARP inhibitor resistance through interacting with PARP1 in ovarian cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHanlin Ma,Gonghua Qi,Fang Han,Wei Lu,Jiali Peng,Rongrong Li,Shi Yan,Cunzhong Yuan,Beihua KongPubMed 35332131
- BMC pulmonary medicine 21:1912021Down-regulation of SNHG16 alleviates the acute lung injury in sepsis rats through miR-128-3p/HMGB3 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunli Sun,Keke Xin,Chenghui Leng,Jianlin GePubMed 34092219
- Cell death & disease 12:5542021Nuclear exosome HMGB3 secreted by nasopharyngeal carcinoma cells promotes tumour metastasis by inducing angiogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaiwen Zhang,Dong Liu,Jianmei Zhao,Si Shi,Xin He,Peng Da,Yiwen You,Bo YouPubMed 34050127
- Aging 13:14304-143212021Accumulation of CD45RO+CD8+ T cells is a diagnostic and prognostic biomarker for clear cell renal cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKe Wu,Xinyi Zheng,Zhixian Yao,Zhong Zheng,Wenjie Huang,Xingyu Mu,Feng Sun,Zhihong Liu,Junhua ZhengPubMed 34016791
- Cancer discovery 11:2638-26572021Cancer Cells Retrace a Stepwise Differentiation Program during Malignant Progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSadegh Saghafinia,Krisztian Homicsko,Annunziata Di Domenico,Stephan Wullschleger,Aurel Perren,Ilaria Marinoni,Giovanni Ciriello,Iacovos P Michael,Douglas HanahanPubMed 33910926
- Medical science monitor : international medical jo 26:e9224242020Silencing of Long Non-Coding RNA X Inactive Specific Transcript (Xist) Contributes to Suppression of Bronchopulmonary Dysplasia Induced by Hyperoxia in Newborn Mice via microRNA-101-3p and the transforming growth factor-beta 1 (TGF-β1)/Smad3 Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenhao Yuan et. al.PubMed 33070148
- Technology in cancer research & treatment 17:15330338188064752018Upregulation of miR-200b Inhibits Hepatocellular Carcinoma Cell Proliferation and Migration by Targeting HMGB3 Protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLong-Kun Wang,Xi-Na Xie,Xu-Hong Song,Ting Su,Xiao-Lan Chang,Man Xu,Bin Liang,Dong-Yang HuangPubMed 30343649
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested | Tested | Expected |
| Rat | Expected | Expected | Tested | Tested | Expected |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. Associates with chromatin and binds DNA with a preference for non-canonical DNA structures such as single-stranded DNA. Can bend DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters (By similarity). Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor (By similarity). Negatively regulates B-cell and myeloid cell differentiation. In hematopoietic stem cells may regulate the balance between self-renewal and differentiation. Involved in negative regulation of canonical Wnt signaling (By similarity).
Alternative names
HMG2A, HMG4, HMGB3, High mobility group protein B3, High mobility group protein 2a, High mobility group protein 4, HMG-2a, HMG-4
Recommended products
Rabbit Recombinant Monoclonal HMG4 antibody. Suitable for ICC/IF, IP, WB, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 8 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP2839Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Western blot - Anti-HMG4 antibody [EP2839Y] (ab75782), expandable thumbnail](https://content.abcam.com/products/images/hmg4-antibody-ep2839y-ab75782--western-blot-img66463.jpg "Western blot - Anti-HMG4 antibody [EP2839Y] (ab75782)")
Western blot - Anti-HMG4 antibody [EP2839Y] (ab75782)
Blocking buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-HMG4 antibody [EP2839Y] (ab75782) at 1/1000 dilution
Lane 1: Human bladder lysate at 20 µg
Lane 2: Human placenta lysate at 20 µg
Lane 3: Mouse brain lysate at 20 µg
Lane 4: Mouse bladder lysate at 20 µg
Lane 5: Rat brain lysate at 20 µg
Lane 6: Rat bladder lysate at 20 µg
Lane 7: Rat placenta lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 25 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMG4 antibody [EP2839Y] (ab75782), expandable thumbnail](https://content.abcam.com/products/images/hmg4-antibody-ep2839y-ab75782--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131105.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMG4 antibody [EP2839Y] (ab75782)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMG4 antibody [EP2839Y] (ab75782)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue sections labeling HMG4 with Purified ab75782 at 1/100 dilution (1.13 μg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
![Immunoprecipitation - Anti-HMG4 antibody [EP2839Y] (ab75782), expandable thumbnail](https://content.abcam.com/products/images/hmg4-antibody-ep2839y-ab75782--immunoprecipitation-img7796.jpg "Immunoprecipitation - Anti-HMG4 antibody [EP2839Y] (ab75782)")
Immunoprecipitation - Anti-HMG4 antibody [EP2839Y] (ab75782)
Purified ab75782 at 1/20 dilution (0.5μg) immunoprecipitating HMG4 in HeLa whole cell lysate.
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
Lane 2: ab75782 + HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab75782 in HeLa whole cell lysate.VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.Observed band size: 25 kDa
All lanes: Immunoprecipitation - Anti-HMG4 antibody [EP2839Y] (ab75782)
Predicted band size: 23 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-HMG4 antibody [EP2839Y] (ab75782), expandable thumbnail](https://content.abcam.com/products/images/hmg4-antibody-ep2839y-ab75782--immunocytochemistry-immunofluorescence-img68409.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HMG4 antibody [EP2839Y] (ab75782)")
Immunocytochemistry/ Immunofluorescence - Anti-HMG4 antibody [EP2839Y] (ab75782)
Immunocytochemistry analysis of 293T (Human embryonic kidney epithelial cell) cells labeling HMG4 with Purified ab75782 at 1/250 dilution (0.452 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
![Flow Cytometry (Intracellular) - Anti-HMG4 antibody [EP2839Y] (ab75782), expandable thumbnail](https://content.abcam.com/products/images/hmg4-antibody-ep2839y-ab75782--flow-cytometry-intracellular-img78736.jpg "Flow Cytometry (Intracellular) - Anti-HMG4 antibody [EP2839Y] (ab75782)")
Flow Cytometry (Intracellular) - Anti-HMG4 antibody [EP2839Y] (ab75782)
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling HMG4 with Purified ab75782 at 1/20 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
![Western blot - Anti-HMG4 antibody [EP2839Y] (ab75782), expandable thumbnail](https://content.abcam.com/products/images/hmg4-antibody-ep2839y-ab75782--western-blot-img28138.jpg "Western blot - Anti-HMG4 antibody [EP2839Y] (ab75782)")
Western blot - Anti-HMG4 antibody [EP2839Y] (ab75782)
Blocking buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-HMG4 antibody [EP2839Y] (ab75782) at 1/5000 dilution
All lanes: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 25 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMG4 antibody [EP2839Y] (ab75782), expandable thumbnail](https://content.abcam.com/products/images/hmg4-antibody-ep2839y-ab75782--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img31478.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMG4 antibody [EP2839Y] (ab75782)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMG4 antibody [EP2839Y] (ab75782)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling HMG4 with Purified ab75782 at 1/100 dilution (1.13 μg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMG4 antibody [EP2839Y] (ab75782), expandable thumbnail](https://content.abcam.com/products/images/hmg4-antibody-ep2839y-ab75782--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img49541.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMG4 antibody [EP2839Y] (ab75782)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMG4 antibody [EP2839Y] (ab75782)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling HMG4 with Purified ab75782 at 1/100 dilution (1.13 μg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
![Western blot - Anti-HMG4 antibody [EP2839Y] (ab75782), expandable thumbnail](https://content.abcam.com/products/images/hmg4-antibody-ep2839y-ab75782--western-blot-img28138.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HMG4 antibody [EP2839Y] (ab75782)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMG4 antibody [EP2839Y] (ab75782), expandable thumbnail](https://content.abcam.com/products/images/hmg4-antibody-ep2839y-ab75782--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img31478.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMG4 antibody [EP2839Y] (ab75782)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMG4 antibody [EP2839Y] (ab75782), expandable thumbnail](https://content.abcam.com/products/images/hmg4-antibody-ep2839y-ab75782--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img49541.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMG4 antibody [EP2839Y] (ab75782)")
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