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Rabbit Recombinant Monoclonal HMGA1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-HMGA1 antibody [EPR16649] (AB202070), expandable thumbnail
  • Western blot - Anti-HMGA1 antibody [EPR16649] (AB202070), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-HMGA1 antibody [EPR16649] (AB202070), expandable thumbnail
  • Western blot - Anti-HMGA1 antibody [EPR16649] (AB202070), expandable thumbnail
  • Western blot - Anti-HMGA1 antibody [EPR16649] (AB202070), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/10000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Tested
Tested

Species
Human
Dilution info
1/150
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double-stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.

Alternative names

HMGIY, HMGA1, High mobility group protein HMG-I/HMG-Y, HMG-I(Y), High mobility group AT-hook protein 1, High mobility group protein R, High mobility group protein A1

Recommended products

Rabbit Recombinant Monoclonal HMGA1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR16649
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-HMGA1 antibody [EPR16649] (ab202070), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-HMGA1 antibody [EPR16649] (ab202070)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized BxPC-3 (Human pancreas adenocarcinoma cells) cells labeling HMGA1 with ab202070 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on BxPC-3 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab202070 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Western blot - Anti-HMGA1 antibody [EPR16649] (ab202070), expandable thumbnail

    Western blot - Anti-HMGA1 antibody [EPR16649] (ab202070)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-HMGA1 antibody [EPR16649] (ab202070) at 1/10000 dilution

    Lane 1: NCCIT (Human pluripotent embryonal carcinoma) whole cell lysate at 10 µg

    Lane 2: BxPC-3 (Human pancreas adenocarcinoma cells) whole cell lysate at 10 µg

    Lane 3: HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 10 µg

    Lane 4: SK-OV-3 (Human ovarian cancer cell line) whole cell lysate at 10 µg

    Lane 5: Caco-2 (Human colorectal adenocarcinoma cells) whole cell lysate at 10 µg

    Lane 6: Human testis tissue lysate at 10 µg

    Lane 7: Human lung tissue lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 12 kDa

    Observed band size: 18 kDa

  • Flow Cytometry (Intracellular) - Anti-HMGA1 antibody [EPR16649] (ab202070), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-HMGA1 antibody [EPR16649] (ab202070)

    Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling HMGA1 with ab202070 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • Western blot - Anti-HMGA1 antibody [EPR16649] (ab202070), expandable thumbnail

    Western blot - Anti-HMGA1 antibody [EPR16649] (ab202070)

    Blocking buffer and concentration: 5% NFDM/TBST
    Diluting buffer and concentration: 5% NFDM/TBST
    We recommend using 1% SDS Hot lysis prepare method to get desired Western Blot results.

    Lanes 1 - 4: Western blot - Anti-HMGA1 antibody [EPR16649] - BSA and Azide free (Anti-HMGA1 antibody [EPR16649] - BSA and Azide free ab251363) at 1/10000 dilution

    Lanes 1 - 4: Western blot - Anti-HMGA1 antibody [EPR16649] (ab202070) at 1/10000 dilution

    Lane 1: HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysate prepared in RIPA lysis method at 20 µg

    Lane 2: HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysate prepared in 1% SDS Hot lysis method at 20 µg

    Lane 3: MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate prepared in RIPA lysis method at 20 µg

    Lane 4: MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate prepared in 1%SDS Hot lysis method at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 12 kDa

    Observed band size: 17 kDa

    Exposure time: 60s

  • Western blot - Anti-HMGA1 antibody [EPR16649] (ab202070), expandable thumbnail

    Western blot - Anti-HMGA1 antibody [EPR16649] (ab202070)

    Blocking buffer and concentration: 5% NFDM/TBST
    Diluting buffer and concentration: 5% NFDM/TBST

    Lanes 1 - 3: Western blot - Anti-HMGA1 antibody [EPR16649] - BSA and Azide free (Anti-HMGA1 antibody [EPR16649] - BSA and Azide free ab251363) at 1/10000 dilution

    Lanes 1 - 3: Western blot - Anti-HMGA1 antibody [EPR16649] (ab202070) at 1/10000 dilution

    Lane 1: MDA-MB-231 (Human breast adenocarcinoma epithelial cell) nuclear fraction lysate at 15 µg

    Lane 2: MDA-MB-231 (Human breast adenocarcinoma epithelial cell) cytoplasm fraction lysate at 15 µg

    Lane 3: MDA-MB-231 (Human breast adenocarcinoma epithelial cell) membrane fraction lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 12 kDa

    Observed band size: 17 kDa

    Exposure time: 10s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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