Anti-HMGA1 antibody [RM1061] (ab315350)

Rabbit Recombinant Multiclonal HMGA1 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Rat, Mouse samples.

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Images

Western blot - Anti-HMGA1 antibody [RM1061] (AB315350), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Multiclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	ICC/IF	Flow Cyt (Intra)	ChIP-seq	IHC-P	ChIC/CUT&RUN-seq
Human	Tested	Tested	Tested	Not recommended	Not recommended	Tested	Not recommended
Mouse	Not recommended	Tested	Expected	Not recommended	Not recommended	Tested	Not recommended
Rat	Expected	Expected	Expected	Not recommended	Not recommended	Tested	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/2000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Target data

See full target information HMGA1

Function

HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double-stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.

Alternative names

HMGIY, HMGA1, High mobility group protein HMG-I/HMG-Y, HMG-I(Y), High mobility group AT-hook protein 1, High mobility group protein R, High mobility group protein A1

Recommended products

Rabbit Recombinant Multiclonal HMGA1 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Rat, Mouse samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Multiclonal
Immunogens: The exact immunogen used to generate this antibody is proprietary information.
Clone number: RM1061
Purification technique: Affinity purification Protein A
Specificity: Unsuitable for mouse IP
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

This product is a recombinant multiclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Western blot - Anti-HMGA1 antibody [RM1061] (ab315350)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: 293T (PMID:27445976), MCF7 (PMID:12640109).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lane 1: 180 seconds Lane 2-6: 81 seconds
All lanes: Western blot - Anti-HMGA1 antibody [RM1061] (ab315350) at 1/1000 dilution
Lane 1: NCCIT (human pluripotent embryonic carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 3: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: HCT-15 (human colon epithelial cell) whole cell lysate at 20 µg
Lane 5: HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 6: NCI-H1299 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 17 kDa, 36 kDa
Immunocytochemistry/ Immunofluorescence - Anti-HMGA1 antibody [RM1061] (ab315350)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NCCIT (human pluripotent embryonic carcinoma epithelial cell) cells labelling HMGA1 with ab315350 at 1/2000 (1.004 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).
Confocal image showing nuclear staining in NCCIT cell line.

Low expression：MCF7 (PMID:12640109).

Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.
Western blot - Anti-HMGA1 antibody [RM1061] (ab315350)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lane 1: 103 seconds Lane 2: 59 seconds Lane 3: 180 seconds
All lanes: Western blot - Anti-HMGA1 antibody [RM1061] (ab315350) at 1/1000 dilution
Lane 1: EL4 (mouse lymphoma T lymphocyte) nuclear fraction at 48 µg
Lane 2: C2C12 (mouse myoblast) nuclear fraction at 48 µg
Lane 3: EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 17 kDa, 36 kDa, 15 kDa
Western blot - Anti-HMGA1 antibody [RM1061] (ab315350)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-HMGA1 antibody [RM1061] (ab315350) at 1/1000 dilution
Lane 1: BxPC-3 (human pancreas adenocarcinoma epithelial cell) nuclear fraction at 20 µg
Lane 2: BxPC-3 (human pancreas adenocarcinoma epithelial cell) cytoplasmic fraction at 20 µg
Lane 3: BxPC-3 (human pancreas adenocarcinoma epithelial cell) membrane fraction at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 17 kDa, 36 kDa, 15 kDa, 125 kDa
Exposure time: 92s
Western blot - Anti-HMGA1 antibody [RM1061] (ab315350)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: MCF7 (PMID:12640109).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-HMGA1 antibody [RM1061] (ab315350) at 1/1000 dilution
Lane 1: BxPC-3 (human pancreas adenocarcinoma epithelial cell) whole cell lysate at 40 µg
Lane 2: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 40 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 17 kDa, 36 kDa
Exposure time: 26s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA1 antibody [RM1061] (ab315350)
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling HMGA1 with ab315350 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Nuclear staining on human ovarian cancer.

The section was incubated with ab315350 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA1 antibody [RM1061] (ab315350)
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling HMGA1 with ab315350 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Nuclear staining on human testis in (A) ab315350 and (B) Anti-HMGA1a / HMGA1b antibody ab4078.

The image on (A) ab315350 is applied with Anti-HMGA1 antibody at 1/2000 dilution and (B) Anti-HMGA1a / HMGA1b antibody ab4078 is applied with Anti-HMGA1 antibody at 1/500 dilution.

The section was incubated with ab315350 or Anti-HMGA1a / HMGA1b antibody ab4078 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA1 antibody [RM1061] (ab315350)
Immunohistochemical analysis of paraffin-embedded Mouse lung carcinoma tissue labeling HMGA1 with ab315350 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse lung carcinoma.

The section was incubated with ab315350 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA1 antibody [RM1061] (ab315350)
Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling HMGA1 with ab315350 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat testis.

The section was incubated with ab315350 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunoprecipitation - Anti-HMGA1 antibody [RM1061] (ab315350)
HMGA1 was immunoprecipitated from 0.35 mg BxPC-3 (human pancreas adenocarcinoma epithelial cell) whole cell lysate with ab315350 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315350 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: BxPC-3 (human pancreas adenocarcinoma epithelial cell) whole cell lysate

Lane 2: ab315350 IP in BxPC-3 (human pancreas adenocarcinoma epithelial cell) whole cell lysate

Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab315350 in BxPC-3 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST
All lanes: Immunoprecipitation - Anti-HMGA1 antibody [RM1061] (ab315350) at 1/30 dilution
All lanes: BxPC-3 (human pancreas adenocarcinoma epithelial cell) whole cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 180s