Anti-HMGA2 antibody (ab97276) is a rabbit polyclonal antibody detecting HMGA2 in Western Blot, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.
- Over 40 publications
- Trusted since 2010
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- Clinical epigenetics 15:922023Histone lysine methyltransferase SMYD3 promotes oral squamous cell carcinoma tumorigenesis via H3K4me3-mediated HMGA2 transcription.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZongcheng Yang,Fen Liu,Zongkai Li,Nianping Liu,Xinfeng Yao,Yu Zhou,Liyu Zhang,Pan Jiang,Honghong Liu,Lingming Kong,Chuandong Lang,Xin Xu,Jihui Jia,Takahito Nakajima,Wenchao Gu,Lixin Zheng,Zhihong ZhangPubMed 37237385
- Biomolecules 13:2023Pivotal Role of Ubiquitin Carboxyl-Terminal Hydrolase L1 (UCHL1) in Uterine Leiomyoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomoo Suzuki,Yidan Dai,Masanori Ono,Junya Kojima,Toru Sasaki,Hiroshi Fujiwara,Naoaki Kuji,Hirotaka NishiPubMed 36830563
- BMC neuroscience 23:492022High-frequency repetitive transcranial magnetic stimulation (rTMS) protects against ischemic stroke by inhibiting M1 microglia polarization through let-7b-5p/HMGA2/NF-κB signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYe Hong,Jinfeng Lyu,Lin Zhu,Xixi Wang,Mengna Peng,Xiangliang Chen,Qiwen Deng,Jie Gao,Zhenhua Yuan,Di Wang,Gelin Xu,Mengyi XuPubMed 35927640
- Journal of diabetes investigation 13:1303-13152022Circular ribonucleic acid nucleoporin 98 knockdown alleviates high glucose-induced proliferation, fibrosis, inflammation and oxidative stress in human glomerular mesangial cells by regulating the microribonucleic acid-151-3p-high mobility group AT-hook 2 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQianlan Dong,Longhao Dong,Yanting Zhu,Xiaoming Wang,Zhenjiang Li,Linping ZhangPubMed 35482475
- Cancer gene therapy 29:1439-14512022Exosomal HMGA2 protein from EBV-positive NPC cells destroys vascular endothelial barriers and induces endothelial-to-mesenchymal transition to promote metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeng-Ke Li,Xing-Rui Chen,Li-Na Wang,Jia-Hong Wang,Ji-Ke Li,Zi-Ying Zhou,Xin Li,Lin-Bo Cai,Shui-Sheng Zhong,Jing-Jing Zhang,Yu-Mei Zeng,Qian-Bing Zhang,Xiao-Yan Fu,Xiao-Ming Lyu,Min-Ying Li,Zhong-Xi Huang,Kai-Tai YaoPubMed 35388172
- Molecular medicine reports 25:2021HMGA2 promotes the migration and invasion of gallbladder cancer cells and HMGA2 knockdown inhibits angiogenesis via targeting VEGFA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Yan,Peng Dai,Xueliang Qin,Yanping He,Yu ZhangPubMed 34913073
- Cancers 13:2021HMGA2 Supports Cancer Hallmarks in Triple-Negative Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBehzad Mansoori,Mikkel Green Terp,Ali Mohammadi,Christina Bøg Pedersen,Henrik Jørn Ditzel,Behzad Baradaran,Morten Frier GjerstorffPubMed 34680349
- Neoplasma 68:1225-12352021LncRNA LINC00355 promotes EMT and metastasis of bladder cancer cells through the miR-424-5p/HMGA2 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen-Jie Li,Gang Li,Zhong-Wen Liu,Zhi-Yong Chen,Rong PuPubMed 34641698
- International journal of cancer 149:1670-16822021Definitive evidence for Club cells as progenitors for mutant Kras/Trp53-deficient lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSebastian Rosigkeit,Marie Kruchem,Dorothe Thies,Andreas Kreft,Emma Eichler,Sebastian Boegel,Sandrine Jansky,Dominik Siegl,Leonard Kaps,Geethanjali Pickert,Patricia Haehnel,Thomas Kindler,Udo F Hartwig,Carmen Guerra,Mariano Barbacid,Detlef Schuppan,Ernesto BockampPubMed 34331774
- BMC endocrine disorders 21:1342021LncRNA HCP5 knockdown inhibits high glucose-induced excessive proliferation, fibrosis and inflammation of human glomerular mesangial cells by regulating the miR-93-5p/HMGA2 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuan Wang,Yan Liu,Jian Rong,Kai WangPubMed 34187448
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human HMGA2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P52926
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Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Tested | Tested | Expected |
| Rat | Expected | Tested | Tested | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/50000 | Notes - |
Species Rat | Dilution info 1/500 - 1/50000 | Notes - |
Species Human | Dilution info 1/500 - 1/50000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
Species Rat | Dilution info 1/100 - 1/1000 | Notes - |
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. Plays an important role in chromosome condensation during the meiotic G2/M transition of spermatocytes. Plays a role in postnatal myogenesis, is involved in satellite cell activation (By similarity). Positively regulates IGF2 expression through PLAG1 and in a PLAG1-independent manner (PubMed:28796236).
Alternative names
HMGIC, HMGA2, High mobility group protein HMGI-C, High mobility group AT-hook protein 2
Recommended products
Anti-HMGA2 antibody (ab97276) is a rabbit polyclonal antibody detecting HMGA2 in Western Blot, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.
- Over 40 publications
- Trusted since 2010
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human HMGA2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P52926
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-HMGA2 antibody (ab97276) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of HMGA2?
Anti-HMGA2 (ab97276) specifically detects a band for HMGA2 (UniProt: P52926) at a molecular weight of 12kDa.
Trusted by the scientific community
Anti-HMGA2 (ab97276) was first used in a scientific publication in 2010 and has been cited over 40 times in peer-reviewed journals.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
17 product images
Western blot - Anti-HMGA2 antibody (ab97276)
15% SDS-PAGE
All lanes: Western blot - Anti-HMGA2 antibody (ab97276) at 1/1000 dilution
Lane 1: NIH/3T3 whole cell extract at 30 µg
Lane 2: Raw264.7 whole cell extract at 30 µg
Lane 3: C2C12 whole cell extract at 30 µg
Predicted band size: 12 kDa
Western blot - Anti-HMGA2 antibody (ab97276)
15% SDS-PAGE
All lanes: Western blot - Anti-HMGA2 antibody (ab97276) at 1/10000 dilution
Lane 1: A431 whole cell extract at 30 µg
Lane 2: HeLa whole cell extract at 30 µg
Lane 3: HepG2 whole cell extract at 30 µg
Predicted band size: 12 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (ab97276)
Paraffin embedded C2C12 xenograft tissue stained for HMGA2 using ab97276 at 1/500 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody (ab97276)
HeLa cells stained for HMGA2 (green) using ab97276 at 1/500 dilution in ICC/IF.
This image is courtesy of an anonymous customer review.Western blot - Anti-HMGA2 antibody (ab97276)
All lanes: Western blot - Anti-HMGA2 antibody (ab97276) at 1/1000 dilution
All lanes: HeLa whole cell lysate at 25 µg
SecondaryAll lanes: Goat anti rabbit secondary IRDye 800CW at 1/1000 dilution
Predicted band size: 12 kDa
Observed band size: 18 kDa
Exposure time: 10min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (ab97276)
Paraffin embedded human HuCCT1 xenograft tissue stained for HMGA2 using ab97276 at 1/250 dilution in immunohistochemical analysis.
Western blot - Anti-HMGA2 antibody (ab97276)
15% SDS-PAGE
All lanes: Western blot - Anti-HMGA2 antibody (ab97276) at 1/1000 dilution
Lane 1: PC-12 whole cell extract at 30 µg
Lane 2: Rat2 whole cell extract at 30 µg
Predicted band size: 12 kDa
Immunoprecipitation - Anti-HMGA2 antibody (ab97276)
Immunoprecipitation of HMGA2 protein from HepG2 nuclear extracts using 5 μg of HMGA2 antibody. Western blot analysis was performed using HMGA2 antibody at 1/500 dilution. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Input
Lane 2: Immunoprecipitation using control IgG
Lane 3: Immunoprecipitation using 5 μg ab97276
All lanes: Immunoprecipitation - Anti-HMGA2 antibody (ab97276)
Predicted band size: 12 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (ab97276)
Paraffin embedded rat duodenum tissue stained for HMGA2 using ab97276 at 1/500 dilution in immunohistochemical analysis.
Western blot - Anti-HMGA2 antibody (ab97276)
15% SDS-PAGE
All lanes: Western blot - Anti-HMGA2 antibody (ab97276) at 1/10000 dilution
All lanes: HCT116 whole cell extract at 30 µg
Predicted band size: 12 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (ab97276)
Paraffin embedded rat brain tissue stained for HMGA2 using ab97276 at 1/500 dilution in immunohistochemical analysis.
Western blot - Anti-HMGA2 antibody (ab97276)
15% SDS PAGE
All lanes: Western blot - Anti-HMGA2 antibody (ab97276) at 1/1000 dilution
All lanes: MOLT-4 whole cell lysate at 30 µg
Predicted band size: 12 kDa
Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody (ab97276)
ICC/IF image of ab97276 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97276, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
Western blot - Anti-HMGA2 antibody (ab97276)
All lanes: Western blot - Anti-HMGA2 antibody (ab97276) at 1/10000 dilution
Lane 1: HepG2 at 30 µg
Lane 2: HCT116 at 30 µg
Predicted band size: 12 kDa
Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody (ab97276)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling HMGA2 with Anti-HMGA2 antibody [RM1215] ab320849 at 1/5000 (0.10 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing nuclear staining in HepG2 cell line
(shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Low expression: HT-29 (PMID: 26964871).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Left Panel is applied with Anti-HMGA2 antibody [RM1215] (Anti-HMGA2 antibody [RM1215] ab320849) at 1/5000 dilution (0.10 ug/ml);
Right panel is applied with Anti-HMGA2 antibody (ab97276) at 1/1000 dilution (0.08 ug/ml).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (ab97276)
Paraffin-embedded rat duodenum and rat brain tissue stained for HMGA2 - ChIP grade protein with ab97276 at a 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunoprecipitation - Anti-HMGA2 antibody (ab97276)
Paraffin-embedded mouse duodenum and mouse brain tissue stained for HMGA2 - ChIP grade protein with ab97276 at a 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
All lanes: Immunoprecipitation - Anti-Integrin beta 1/CD29 antibody [HL1255] (Anti-Integrin beta 1/CD29 antibody [HL1255] ab308384)
All lanes: A431 whole cell extracts
SecondaryAll lanes: HRP-conjugated anti rabbit IgG antibody
Predicted band size: 88 kDa
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