Anti-HMGA2 antibody [EPR18114]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR18114] (AB207301)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling HMGA2 with ab207301 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human colon tissue. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR18114] (AB207301)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling HMGA2 with ab207301 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human colon cancer is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR18114] (AB207301)

Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling HMGA2 with ab207301 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human gastric cancer is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

AbReview59874****

Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody [EPR18114] (AB207301)

ab207301 staining HMGA2 in the HeLal cell line from Human Cervix by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldahyde, permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/400 in PBS) for 1 hour at 22°C. ab150081 (1/200)was used as the secondary antibody.

This image is courtesy of an abreview by Kirk Mcmanus

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody [EPR18114] (AB207301)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling HMGA2 with ab207301 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HepG2 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab207301 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody [EPR18114] (AB207301)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling HMGA2 with ab207301 at 1/100 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab207301 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

• WB

Lab

Western blot - Anti-HMGA2 antibody [EPR18114] (AB207301)

All lanes:

Western blot - Anti-HMGA2 antibody [EPR18114] (ab207301) at 1/1000 dilution

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) prepared in RIPA lysis method at 20 µg

Lane 2:

HepG2 (Human hepatocellular carcinoma epithelial cell) prepared in RIPA lysis method at 20 µg

Lane 3:

NCCIT (Human pluripotent embryonic carcinoma epithelial cell) prepared in 1% SDS Hot lysis method at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 12 kDa

Observed band size: 18 kDa

false

• WB

Unknown

Western blot - Anti-HMGA2 antibody [EPR18114] (AB207301)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-HMGA2 antibody [EPR18114] (ab207301) at 1/1000 dilution

Lane 1:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate prepared in RIPA lysis method at 15 µg

Lane 2:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate prepared in 1%SDS Hot lysis method at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 12 kDa

Observed band size: 18 kDa

false

Exposure time: 80s