Rabbit Recombinant Monoclonal HMGA2 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Images
![Immunoprecipitation - Anti-HMGA2 antibody [EPR23215-46] (AB246513), expandable thumbnail](https://content.abcam.com/products/images/hmga2-antibody-epr23215-46-ab246513--immunoprecipitation-img4299.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-HMGA2 antibody [EPR23215-46] (AB246513)")
![Western blot - Anti-HMGA2 antibody [EPR23215-46] (AB246513), expandable thumbnail](https://content.abcam.com/products/images/hmga2-antibody-epr23215-46-ab246513--western-blot-img16233.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HMGA2 antibody [EPR23215-46] (AB246513)")
![Flow Cytometry (Intracellular) - Anti-HMGA2 antibody [EPR23215-46] (AB246513), expandable thumbnail](https://content.abcam.com/products/images/hmga2-antibody-epr23215-46-ab246513--flow-cytometry-intracellular-img27269.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-HMGA2 antibody [EPR23215-46] (AB246513)")
![Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody [EPR23215-46] (AB246513), expandable thumbnail](https://content.abcam.com/products/images/hmga2-antibody-epr23215-46-ab246513--immunocytochemistry-immunofluorescence-img131975.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody [EPR23215-46] (AB246513)")
Publications
Filter by
- OncoTargets and therapy 14:1-132021Long Noncoding RNA CCDC144NL-AS1 Promotes the Oncogenicity of Osteosarcoma by Acting as a Molecular Sponge for microRNA-490-3p and Thereby Increasing HMGA2 Expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuliang He et. al.PubMed 33442262
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/600 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. Plays an important role in chromosome condensation during the meiotic G2/M transition of spermatocytes. Plays a role in postnatal myogenesis, is involved in satellite cell activation (By similarity). Positively regulates IGF2 expression through PLAG1 and in a PLAG1-independent manner (PubMed:28796236).
Alternative names
High mobility group protein HMGI-C, High mobility group AT-hook protein 2, HMGIC, HMGA2
Recommended products
Rabbit Recombinant Monoclonal HMGA2 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Alternative names
High mobility group protein HMGI-C, High mobility group AT-hook protein 2, HMGIC, HMGA2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR23215-46
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunoprecipitation - Anti-HMGA2 antibody [EPR23215-46] (ab246513), expandable thumbnail](https://content.abcam.com/products/images/hmga2-antibody-epr23215-46-ab246513--immunoprecipitation-img4299.jpg "Immunoprecipitation - Anti-HMGA2 antibody [EPR23215-46] (ab246513)")
Immunoprecipitation - Anti-HMGA2 antibody [EPR23215-46] (ab246513)
HMGA2 was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate with ab246513 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246513 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate 10 ug
Lane 2: 246513 IP in HepG2 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab246513 in HepG2 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
All lanes: Immunoprecipitation - Anti-HMGA2 antibody [EPR23215-46] (ab246513)
Predicted band size: 12 kDa
![Western blot - Anti-HMGA2 antibody [EPR23215-46] (ab246513), expandable thumbnail](https://content.abcam.com/products/images/hmga2-antibody-epr23215-46-ab246513--western-blot-img16233.jpg "Western blot - Anti-HMGA2 antibody [EPR23215-46] (ab246513)")
Western blot - Anti-HMGA2 antibody [EPR23215-46] (ab246513)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 28765279)
Exposure time: 3 minutes.
All lanes: Western blot - Anti-HMGA2 antibody [EPR23215-46] (ab246513) at 1/1000 dilution
Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 40 µg
Lane 2: NCI-H1299 (human lung carcinoma epithelial cell), whole cell lysate at 40 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 12 kDa
Observed band size: 18 kDa
![Flow Cytometry (Intracellular) - Anti-HMGA2 antibody [EPR23215-46] (ab246513), expandable thumbnail](https://content.abcam.com/products/images/hmga2-antibody-epr23215-46-ab246513--flow-cytometry-intracellular-img27269.jpg "Flow Cytometry (Intracellular) - Anti-HMGA2 antibody [EPR23215-46] (ab246513)")
Flow Cytometry (Intracellular) - Anti-HMGA2 antibody [EPR23215-46] (ab246513)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cells labelling HMGA2 with ab246513 at 1/600 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
![Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody [EPR23215-46] (ab246513), expandable thumbnail](https://content.abcam.com/products/images/hmga2-antibody-epr23215-46-ab246513--immunocytochemistry-immunofluorescence-img131975.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody [EPR23215-46] (ab246513)")
Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody [EPR23215-46] (ab246513)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 cells labelling HMGA2 with ab246513 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing nuclear staining in HepG2 cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.
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