Rabbit Recombinant Monoclonal HMGA2 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.
Images
![Western blot - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (AB319058), expandable thumbnail](https://content.abcam.com/products/images/hmga2-antibody-epr29217-93-bsa-and-azide-free-ab319058--western-blot-img434416.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (AB319058)")
![Western blot - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (AB319058), expandable thumbnail](https://content.abcam.com/products/images/hmga2-antibody-epr29217-93-bsa-and-azide-free-ab319058--western-blot-img434414.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (AB319058)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (AB319058), expandable thumbnail](https://content.abcam.com/products/images/hmga2-antibody-epr29217-93-bsa-and-azide-free-ab319058--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img434497.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (AB319058)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (AB319058), expandable thumbnail](https://content.abcam.com/products/images/hmga2-antibody-epr29217-93-bsa-and-azide-free-ab319058--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img434496.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (AB319058)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (AB319058), expandable thumbnail](https://content.abcam.com/products/images/hmga2-antibody-epr29217-93-bsa-and-azide-free-ab319058--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img434495.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (AB319058)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Tested | Tested |
| Rat | Expected | Tested |
| Recombinant fragment - Human | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human, Human, Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Target data
Function
Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. Plays an important role in chromosome condensation during the meiotic G2/M transition of spermatocytes. Plays a role in postnatal myogenesis, is involved in satellite cell activation (PubMed:27446912). Positively regulates IGF2 expression through PLAG1 and in a PLAG1-independent manner (By similarity).
Alternative names
Hmgic, High mobility group protein HMGI-C, High mobility group AT-hook protein 2
Recommended products
Rabbit Recombinant Monoclonal HMGA2 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR29217-93
- Purification technique
- Affinity purification Protein A
- Specificity
For WB testing, 1% SDS HOT lysis method for cell lines/tissues is recommended.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab319058 is the carrier-free version of Anti-HMGA2 antibody [EPR29217-93] ab319057.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (ab319058), expandable thumbnail](https://content.abcam.com/products/images/hmga2-antibody-epr29217-93-bsa-and-azide-free-ab319058--western-blot-img434416.jpg "Western blot - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (ab319058)")
Western blot - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (ab319058)
This data was developed using Anti-HMGA2 antibody [EPR29217-93] ab319057, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody does not cross-react with human HMGA1.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-HMGA2 antibody [EPR29217-93] (Anti-HMGA2 antibody [EPR29217-93] ab319057) at 1/1000 dilution
Lane 1: His tagged human HMGA2 fragment at 10 ng
Lane 2: His tagged human HMGA1 fragment at 40 ng
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 36 kDa
Exposure time: 26s
![Western blot - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (ab319058), expandable thumbnail](https://content.abcam.com/products/images/hmga2-antibody-epr29217-93-bsa-and-azide-free-ab319058--western-blot-img434414.jpg "Western blot - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (ab319058)")
Western blot - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (ab319058)
This data was developed using Anti-HMGA2 antibody [EPR29217-93] ab319057, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: liver (PIMD: 7651535)
Lane 1 and lane 2 samples were prepared via the 1% SDS HOT lysis method(100℃, 30 min), lane 3 and lane 5 were prepared via RIPA lysis.
The lysate of lane 5 was freshly made and used immediately to minimize protein degradation.
The identity of the higher MW band at approximately 37 kDa (in lane 4) is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-HMGA2 antibody [EPR29217-93] (Anti-HMGA2 antibody [EPR29217-93] ab319057) at 1/1000 dilution
Lane 1: Mouse E12 embryo tissue (HOT) lysate at 20 µg
Lane 2: Mouse liver tissue (HOT) lysate at 20 µg
Lane 3: Mouse E12 embryo tissue (RIPA) lysate at 20 µg
Lane 4: Mouse liver tissue (RIPA) lysate at 20 µg
Lane 5: NIH/3T3 (mouse embryonic fibroblast) whole (RIPA) cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 18 kDa, 36 kDa
Exposure time: 26s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (ab319058), expandable thumbnail](https://content.abcam.com/products/images/hmga2-antibody-epr29217-93-bsa-and-azide-free-ab319058--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img434497.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (ab319058)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (ab319058)
This data was developed using Anti-HMGA2 antibody [EPR29217-93] ab319057, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling HMGA2 with Anti-HMGA2 antibody [EPR29217-93] ab319057 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression: weak staining on mouse liver.
The section was incubated with Anti-HMGA2 antibody [EPR29217-93] ab319057 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the backgroundCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (ab319058), expandable thumbnail](https://content.abcam.com/products/images/hmga2-antibody-epr29217-93-bsa-and-azide-free-ab319058--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img434496.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (ab319058)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (ab319058)
This data was developed using Anti-HMGA2 antibody [EPR29217-93] ab319057, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat E12.5 tissue labeling HMGA2 with Anti-HMGA2 antibody [EPR29217-93] ab319057 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat E12.5.
The section was incubated with Anti-HMGA2 antibody [EPR29217-93] ab319057 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the backgroundCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (ab319058), expandable thumbnail](https://content.abcam.com/products/images/hmga2-antibody-epr29217-93-bsa-and-azide-free-ab319058--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img434495.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (ab319058)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (ab319058)
This data was developed using Anti-HMGA2 antibody [EPR29217-93] ab319057, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse E12.5 tissue labeling HMGA2 with Anti-HMGA2 antibody [EPR29217-93] ab319057 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse E12.5 (PMID: 7651535).
The section was incubated with Anti-HMGA2 antibody [EPR29217-93] ab319057 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the backgroundCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (ab319058), expandable thumbnail](https://content.abcam.com/products/images/hmga2-antibody-epr29217-93-bsa-and-azide-free-ab319058--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img434498.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (ab319058)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (ab319058)
This data was developed using Anti-HMGA2 antibody [EPR29217-93] ab319057, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling HMGA2 with Anti-HMGA2 antibody [EPR29217-93] ab319057 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression: weak staining on rat liver.
The section was incubated with Anti-HMGA2 antibody [EPR29217-93] ab319057 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the backgroundCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Western blot - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (ab319058), expandable thumbnail](https://content.abcam.com/products/images/hmga2-antibody-epr29217-93-bsa-and-azide-free-ab319058--western-blot-img434415.jpg "Western blot - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (ab319058)")
Western blot - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (ab319058)
This data was developed using Anti-HMGA2 antibody [EPR29217-93] ab319057, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Lane 3 sample was prepared via the 1% SDS HOT lysis method, lane 1 and lane 2 samples were prepared via RIPA lysis.
The identity of the higher MW band at approximately 37 kDa (in lane 3) is unknown.
All lanes: Western blot - Anti-HMGA2 antibody [EPR29217-93] (Anti-HMGA2 antibody [EPR29217-93] ab319057) at 1/1000 dilution
Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole (RIPA) cell lysate at 20 µg
Lane 2: NCCIT (human pluripotent embryonic carcinoma epithelial cell) whole (RIPA) cell lysate at 20 µg
Lane 3: HepG2 (human hepatocellular carcinoma epithelial cell) whole (HOT) cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 18 kDa
Exposure time: 180s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (ab319058), expandable thumbnail](https://content.abcam.com/products/images/hmga2-antibody-epr29217-93-bsa-and-azide-free-ab319058--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img434498.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (ab319058)")
![Western blot - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (ab319058), expandable thumbnail](https://content.abcam.com/products/images/hmga2-antibody-epr29217-93-bsa-and-azide-free-ab319058--western-blot-img434415.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HMGA2 antibody [EPR29217-93] - BSA and Azide free (ab319058)")
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